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GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: a localization study with antibodies and riboprobes to GnRH-associated peptides

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Abstract

The distribution of cells that express three prepro-gonadotropin-releasing hormones (GnRH), corresponding to salmon GnRH, sea bream GnRH (sbGnRH), and chicken II GnRH, was studied in the brain and pituitary of the South American cichlid fish, Cichlasoma dimerus. Although the ontogeny and distribution of GnRH neuronal systems have previously been examined immunohistochemically with antibodies and antisera against the various GnRH decapeptides, we have used antisera against various perciform GnRH-associated peptides (GAPs) and riboprobes to various perciform GnRH+GAPs. The results demonstrate that: (1) the GnRH neuronal populations in the forebrain (salmon and sea bream GAPs; sGAP and sbGAP, respectively) show an overlapping pattern along the olfactory bulbs, nucleus olfacto-retinalis, ventral telencephalon, and preoptic area; (2) projections with sGAP are mainly located in the forebrain and contribute to the pituitary innervation, with projections containing chicken GAP II being mainly distributed along the mid and hindbrain and not contributing to pituitary innervation, whereas sbGAP projections are restricted to the ventral forebrain, being the most important molecular form in relation to pituitary innervation; (3) sbGnRH (GnRH I) neurons have an olfactory origin; (4) GAP antibodies and GAP riboprobes are valuable tools for the study of various GnRH systems, by avoiding the cross-reactivity problems that occur when using GnRH antibodies and GnRH riboprobes alone.

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Authors and Affiliations

  1. Laboratorio de Embriología Animal, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Buenos Aires, C1428EHA, Argentina

    Matías Pandolfi, Fabiana L. Lo Nostro & Maria Cristina Maggese

  2. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Puerto Real, 11510, Spain

    José Antonio Muñoz Cueto

  3. Laboratorio de Biología del Desarrollo, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. Ciudad Universitaria, Pabellón 2, Buenos Aires, C1428EHA, Argentina

    Dante A. Paz

  4. Division of Neuroscience, Oregon National Primate Research Center, Oregon Health and Sciences University, Beaverton, Ore., 97201, USA

    Matías Pandolfi, Jodi L. Downs & Henryk F. Urbanski

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  1. Matías Pandolfi
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  2. José Antonio Muñoz Cueto
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  3. Fabiana L. Lo Nostro
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  4. Jodi L. Downs
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  5. Dante A. Paz
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  6. Maria Cristina Maggese
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  7. Henryk F. Urbanski
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Correspondence to Matías Pandolfi.

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This work was supported by grants UBACYT X-217, Conicet PIP 0539/R188; NIH-HD-29186 and Fogarty International Fellowship TW00086.

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Pandolfi, M., Muñoz Cueto, J.A., Lo Nostro, F.L. et al. GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: a localization study with antibodies and riboprobes to GnRH-associated peptides. Cell Tissue Res 321, 219–232 (2005). https://doi.org/10.1007/s00441-004-1055-7

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