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High reactive oxygen species levels are detected at the end of the chronological life span of translocant yeast cells

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Abstract

Chromosome translocation is a major genomic event for a cell, affecting almost every of its life aspects ranging from metabolism, organelle maintenance and homeostasis to gene maintenance and expression. By using the bridge-induced translocation system, we defined the effects of induced chromosome translocation on the chronological life span (CLS) of yeast with particular interest to the oxidative stress condition. The results demonstrate that every translocant strain has a different CLS, but all have a high increase in reactive oxygen species and in lipid peroxides levels at the end of the life span. This could be due to the very unique and strong deregulation of the oxidative stress network. Furthermore, the loss of the translocated chromosome occurs at the end of the life span and is locus dependent. Additionally, the RDH54 gene may play a role in the correct segregation of the translocant chromosome, since in its absence there is an increase in loss of the bridge-induced translocated chromosome.

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Acknowledgments

The authors wish to thank Dmitri Nikitin and Beatrice Rossi for technical help and suggestions and Frank Madeo for making available the facilities for the apoptosis experiments.

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Author notes

  1. Jason Sims

    Present address: Department of Chromosome Biology, Max Perutz Laboratories, Dr. Bohr-Gasse 9, 1030, Vienna, Austria

  2. Carlo V. Bruschi

    Present address: Central European Initiative, Via Genova 9, 34121, Trieste, Italy

Authors and Affiliations

  1. Yeast Molecular Genetics Group, ICGEB, Area Science Park, Padriciano 99, 34149, Trieste, Italy

    Jason Sims, Carlo V. Bruschi, Nicole West & Valentina Tosato

  2. Cellular and Molecular Life Sciences, University of Rennes, 9 Rue Jean Macé, 35700, Rennes, France

    Chloé Bertin

  3. Genetics Division, Department of Cell Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria

    Michael Breitenbach, Sabrina Schroeder & Mark Rinnerthaler

  4. Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 8010, Graz, Austria

    Tobias Eisenberg

  5. Institute of Food, Nutrition and Health, University of Primorska, Polje 42, 6310, Izola, Slovenia

    Peter Raspor

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  1. Jason Sims
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Correspondence to Valentina Tosato.

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VT was supported by Crescendo Biologics Ltd., Cambridge, UK.

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The authors declare no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by S. Hohmann.

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Sims, J., Bruschi, C.V., Bertin, C. et al. High reactive oxygen species levels are detected at the end of the chronological life span of translocant yeast cells. Mol Genet Genomics 291, 423–435 (2016). https://doi.org/10.1007/s00438-015-1120-9

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  • DOI: https://doi.org/10.1007/s00438-015-1120-9

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