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Ascorbate peroxidase gene family in tomato: its identification and characterization

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Abstract

The antioxidative response, where ascorbate peroxidase (APX) is a key enzyme, is an integral part of the plant tolerance response to environmental stresses. As a first step towards the study of the physiological role and the regulation of the members of the Apx gene family, the orthologs of the stress-sensitive cultivated tomato Solanum lycopersicum cv. M82 (Slm) and of the wild salt-tolerant species S. pennellii acc. Atico (Spa) were identified by utilizing the tomato EST database, and characterized. A redundant list of 16 virtual Apx transcripts and four singleton ESTs was shown to correspond to seven genuine Apx genes. The complete tomato Apx gene family is comprised of genes encoding three cytosolic, two peroxisomal, and two chloroplastic APXs. These genes attained differential regulatory patterns in various Slm organs. More detailed study of Apx1 and Apx2 genes, that are the products of a recent gene duplication event, shows that they have already attained differential regulation within and between Slm and Spa under control and stress conditions. It is also suggested that due to lineage-specific gene duplication and lose events, intricate phylogenetic relationships exist among the members of the Apx gene families.

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Acknowledgments

We thank Prof. L. Reinhold for critical reading of the manuscript and many useful comments. The study was supported by Dr. Herman Kessel Research Fund, in the memory of Mr. C. J. J. Van Kensbury.

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Author notes

  1. Tibor Janda

    Present address: Agricultural Research Institute of the Hungarian Academy of Sciences, POB 19, 2462, Martonvásár, Hungary

Authors and Affiliations

  1. Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

    Naim Najami, Waseim Barriah, Galya Kayam & Moshe Tal

  2. The Institutes for Desert Research, The Albert Katz department for Dryland Biotechnologies, Ben-Gurion University of the Negev, Sede Boqer, Israel

    Tibor Janda & Micha Guy

  3. The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, Israel

    Micha Volokita

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  1. Naim Najami
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Correspondence to Micha Volokita.

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Communicated by C. Gebhardt.

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Najami, N., Janda, T., Barriah, W. et al. Ascorbate peroxidase gene family in tomato: its identification and characterization. Mol Genet Genomics 279, 171–182 (2008). https://doi.org/10.1007/s00438-007-0305-2

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  • DOI: https://doi.org/10.1007/s00438-007-0305-2

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