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The GRAS protein SCL13 is a positive regulator of phytochrome-dependent red light signaling, but can also modulate phytochrome A responses

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Abstract

Phytochrome photoreceptors enable plants to perceive divergent light signals leading to adaptive changes in response to differing environmental conditions. However, the mechanism of light signal transduction is not fully understood. Here we report the identification of a new signaling intermediate from Arabidopsis thaliana, Scarecrow-like (SCL)13, which serves as a positive regulator of continuous red light signals downstream of phytochrome B (phyB). SCL13 antisense lines exhibit reduced sensitivity towards red light, but only a distinct subset of phyB-mediated responses is affected, indicating that SCL13 executes its major role in hypocotyl elongation during de-etiolation. Genetic evidence suggests that SCL13 is also needed to modulate phytochrome A (phyA) signal transduction in a phyB-independent way. The SCL13 protein is localized in the cytoplasm, but can also be detected in the nucleus. Overexpression of both a nuclear and cytoplasmic localized SCL13 protein leads to a hypersensitive phenotype under red light indicating that SCL13 is biologically active in both compartments. SCL13 is a member of the plant-specific GRAS protein family, which is involved in various different developmental and signaling pathways. A previously identified phytochrome A signaling intermediate, PAT1, belongs to the same subbranch of GRAS proteins as SCL13. Although both proteins are involved in phytochrome signaling, each is specific for a different light condition and regulates a different subset of responses.

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Acknowledgments

We would like to thank Prof. Dr. R. G Herrmann for his support, Christine Matzenbacher for excellent technical assistance, and Randy Foster for helpful discussions and suggestions. This work was supported by a grant from the “Deutsche Forschungsgemeinschaft” (DFG) to C.B. (Bo1146/3). Work at Rockefeller was supported by NIH grant GM 44640 to N.H.C.

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  1. Department für Biologie I/Bereich Botanik, Ludwig-Maximilians-University, Menzinger Str. 67, 80638, Munich, Germany

    Patricia Torres-Galea & Cordelia Bolle

  2. The Rockefeller University, New York, NY, 10021, USA

    Li-Fang Huang & Nam-Hai Chua

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  1. Patricia Torres-Galea
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  2. Li-Fang Huang
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Correspondence to Cordelia Bolle.

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Communicated by G. Jürgens

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Torres-Galea, P., Huang, LF., Chua, NH. et al. The GRAS protein SCL13 is a positive regulator of phytochrome-dependent red light signaling, but can also modulate phytochrome A responses. Mol Genet Genomics 276, 13–30 (2006). https://doi.org/10.1007/s00438-006-0123-y

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