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Fine mapping of the nematode resistance gene Mi-3 in Solanum peruvianum and construction of a S. lycopersicum DNA contig spanning the locus

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Abstract

Currently, the only genetic resistance against root-knot nematodes in the cultivated tomato Solanum lycopersicum (Lycopersicon esculentum) is due to the gene Mi-1. Another resistance gene, Mi-3, identified in the related wild species Solanum peruvianum (Lycopersicon peruvianum) confers resistance to nematodes that are virulent on tomato lines that carry Mi-1, and is effective at temperatures at which Mi-1 is not effective (above 30°C). Two S. peruvianum populations segregating for Mi-3 were used to develop a high-resolution map of the Mi-3 region of chromosome 12. S. lycopersicum BACs carrying flanking markers were identified and used to construct a contig spanning the Mi-3 region. Markers generated from BAC-end sequences were mapped in S. peruvianum plants in which recombination events had occurred near Mi-3. Comparison of the S. peruvianum genetic map with the physical map of S. lycopersicum indicated that marker order is conserved between S. lycopersicum and S. peruvianum. The 600 kb contig between Mi-3-flanking markers TG180 and NR18 corresponds to a genetic distance of about 7.2 cM in S. peruvianum. We have identified a marker that completely cosegregates with Mi-3, as well as flanking markers within 0.25 cM of the gene. These markers can be used to introduce Mi-3 into cultivated tomato, either by conventional breeding or cloning strategies.

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Acknowledgements

We would like to thank R. Peng, E. Westbrook and W. Pudlo for technical support and S. King for developing the pseudo-F2 population. We thank S. Seah for critical reading of the manuscript. This work was supported by funds from: the National Science Foundation Cooperative Agreement BIR-8920216 to the Center for Engineering Plants for Resistance against Pathogens, an NSF Science and Technology Center; United States Department of Agriculture National Research Initiative Award 2003-00996; the University of California BioSTAR Program and the DNA Plant Technology Corporation. All experiments described in this paper comply with the current laws of the USA.

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  1. J. L. Yates

    Present address: Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Road, Athens, GA, 30602, USA

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  1. Department of Nematology, University of California, Davis, CA, 95616, USA

    J. Yaghoobi, J. L. Yates & V. M. Williamson

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  1. J. Yaghoobi
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Correspondence to V. M. Williamson.

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Communicated by R. McCombie

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Yaghoobi, J., Yates, J.L. & Williamson, V.M. Fine mapping of the nematode resistance gene Mi-3 in Solanum peruvianum and construction of a S. lycopersicum DNA contig spanning the locus. Mol Genet Genomics 274, 60–69 (2005). https://doi.org/10.1007/s00438-005-1149-2

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