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Mapping genes for resistance to Verticillium albo-atrum in tetraploid and diploid potato populations using haplotype association tests and genetic linkage analysis

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Abstract

Verticillium wilt disease of potato is caused predominantly by Verticillium albo-atrum and V. dahliae. StVe1 —a putative QTL for resistance against V. dahliae —was previously mapped to potato chromosome 9. To develop allele-specific, SNP-based markers within the locus, the StVe1 fragment from a set of 30 North American potato cultivars was analyzed. Three distinct and highly diverse haplotypes can be distinguished at the StVe1 locus. These were detected in 97%, 33%, and 10% of the cultivars analyzed. We tested for haplotype association and for genetic linkage between the StVe1 haplotypes and resistance of tetraploid potato to V. albo-atrum. Moreover, field resistance was assessed in diploid populations with known molecular linkage maps in order to identify novel QTLs. Resistance QTLs against V. albo-atrum were detected on four chromosomes (2, 6, 9, and 12) at the diploid level, with one QTL on chromosome 2 contributing over 40% to the total phenotypic variation of the trait. At the tetraploid level, a significant association between the StVe1-839-C haplotype and susceptibility to the disease was detected, suggesting that resistance-related genes directed against V. albo-atrum and V. dahliae are located in the same genomic region of chromosome 9. However, on the basis of the present analysis, we cannot determine whether these genes are closely linked or if a single gene provides resistance against both Verticillium species. To assess the usefulness of the StVe1-839-C haplotype for marker-assisted selection, we subjected the resistance data to Bayesian analysis, and calculated positive (0.65) and negative (0.75) predictive values, and overall predictive accuracy (0.72). Our results indicate that tagging of additional genes for resistance to Verticillium with molecular markers will be required for efficient marker-assisted selection.

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Acknowledgements

We would like to thank Dr. N. Anderson, Dr. J. Bradeen, and Dr. A. Grybauskas for providing Verticillium albo-atrum cultures, Dr. R. Veilleux for monoploid potato genotypes, Dr. S. Jansky and Dr. D. Fravel for sharing their expertise in Verticillium cultivation and identification, D. Hyten for help with data analysis, Dr. M. Ospina-Giraldo for valuable discussion, and Dr. J. Bradeen and Dr. W. De Jong for critically reading the manuscript. This project was supported in part by the ARS potato research program.

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Author notes

  1. S. Costanzo

    Present address: Department of Plant Pathology, Ohio State University, Wooster, OH 44691, USA

Authors and Affiliations

  1. Vegetable Laboratory, USDA-ARS-PSI, Beltsville, MD 20705, USA

    I. Simko, K. G. Haynes & R. W. Jones

  2. Department of Natural Resource Sciences and Landscape Architecture, University of Maryland, College Park, MD 20742, USA

    I. Simko

  3. Department of Horticulture, Cornell University, Ithaca, NY 14853, USA

    E. E. Ewing

  4. Department of Plant Pathology, Penn State University, University Park, PA 16802, USA

    S. Costanzo & B. J. Christ

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  1. I. Simko
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  2. K. G. Haynes
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  4. S. Costanzo
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Correspondence to I. Simko.

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Communicated by M.-A. Grandbastien

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Simko, I., Haynes, K.G., Ewing, E.E. et al. Mapping genes for resistance to Verticillium albo-atrum in tetraploid and diploid potato populations using haplotype association tests and genetic linkage analysis. Mol Genet Genomics 271, 522–531 (2004). https://doi.org/10.1007/s00438-004-1010-z

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  • DOI: https://doi.org/10.1007/s00438-004-1010-z

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