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An integrated physical and genetic map of the nematode Pristionchus pacificus

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Abstract

The free-living nematode Pristionchus pacificus is one of several species that have recently been developed as a satellite system for comparative functional studies in evolutionary developmental biology. Comparisons of developmental processes between P. pacificus and the well established model organism Caenorhabditis elegans at the cellular and genetic levels provide detailed insight into the molecular changes that shape evolutionary transitions. To facilitate genetic analysis and cloning of mutations in P. pacificus, we previously generated a BAC-based genetic linkage map for this organism. Here, we describe the construction of a physical map of the P. pacificus genome based on AFLP fingerprint analysis of 7747 BAC clones. Most of the SSCP markers used to generate the genetic linkage map were derived from BAC ends, so that the physical genome map and the genetic map can be integrated. The contigs that make up the physical map are evenly distributed over the genetic linkage map and no clustering is observed, indicating that the physical map provides a valid representation of the P. pacificus genome. The integrated genome map thus provides a framework for positional cloning and the study of genome evolution in nematodes.

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Acknowledgements

We thank G. Otto, A. Pires-daSilva and K.-Z. Lee for comments on the manuscript. The work described in this manuscript was financially supported by the Max-Planck Society. R. J. S. is a Max-Planck Society Investigator

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Authors and Affiliations

  1. Abteilung für Evolutionsbiologie, Max-Planck Institut für Entwicklungsbiologie, Spemannstrasse 37, 72076, Tübingen, Germany

    J. Srinivasan, W. Sinz & R. J. Sommer

  2. Keygene N.V., Agro Business Park 90, 6708 AE, Wageningen, The Netherlands

    T. Jesse, L. Wiggers-Perebolte, K. Jansen, J. Buntjer & M. van der Meulen

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  1. J. Srinivasan
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  2. W. Sinz
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  3. T. Jesse
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  4. L. Wiggers-Perebolte
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  5. K. Jansen
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  6. J. Buntjer
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  7. M. van der Meulen
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  8. R. J. Sommer
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Correspondence to R. J. Sommer.

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Communicated by G. Jürgens

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Srinivasan, J., Sinz, W., Jesse, T. et al. An integrated physical and genetic map of the nematode Pristionchus pacificus . Mol Gen Genomics 269, 715–722 (2003). https://doi.org/10.1007/s00438-003-0881-8

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  • DOI: https://doi.org/10.1007/s00438-003-0881-8

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