Abstract
In this work, we compare and evaluate the efficiency of fosfatriclaben with three commercial fasciolicides in experimentally infected sheep. Fosfatriclaben is a novel prodrug derived from triclabendazole; it is highly water-soluble with excellent aqueous stability at pH 7, properties that make it ideal for developing intramuscular pharmaceutical compositions in the form of solutions. In order to compare, 30 mixed breed sheep, previously diagnosed negative to fluke eggs, were infected with 200 metacercariae of Fasciola hepatica, twice. Five groups of six animals/each were formed for treatments. Group 1 (G1) was treated with closantel 5% injectable at 5 mg/kg subcutaneously, G2 with clorsulon at 2 mg/kg subcutaneously, G3 with triclabendazole at 12 mg/kg per os, G4 with fosfatriclaben at 6 mg/kg intramuscularly (dose equivalent to triclabendazole content), and G5 remained as the non-treated control. On day 110, fecal samples were examined to determine the percentage of egg reduction after treatment, and sheep were humanely euthanized. The livers were collected, the flukes were extracted, measured, and counted. Efficiency in egg reduction was of 86.8, 90.5, 98.4, and 97.3% for closantel, clorsulon, triclabendazole, and fosfatriclaben, respectively, and efficiency against flukes was of 96.2, 91.9, 99.4, and 95.7%, respectively. No statistical differences were found between treatments. It is concluded that fosfatriclaben at 6 mg/kg intramuscularly presented a high fasciolicide efficiency, similar to the best commercial fasciolicides, having advantage over its predecessor since it uses half of the dose required by triclabendazole to remove flukes in sheep under study.
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Acknowledgments
The authors are indebted to Laboratorios Loeffler, S.A. de C.V. for kind donation of triclabendazole. M. Flores-Ramos is very grateful to Dirección General de Asuntos del Personal Académico (DGAPA-UNAM) for the Postdoctoral fellowship granted.
We thank Dr. Irene Cruz Mendoza of the Laboratory of Parasitology of the Facultad de MedicinaVeterinaria y Zootecnia-UNAM for the provision of metacercariae used in this work.
Funding
This work was supported by the project PAPIIT, DGAPA, UNAM-IT201018.
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All procedures performed in this work are in agreement with the ethical standards of institutional guidelines on the care and use of animals. This experimental protocol was approved by the Comité Interno para el Cuidado y Uso de Animales (CICUA) of the Facultad de Medicina Veterinaria y Zootecnia of the Universidad Nacional Autónoma de México, with the number of protocol MC-2017/2-2.
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Arias-García, R., Vera-Montenegro, Y., Flores-Ramos, M. et al. Efficiency comparison of experimental fosfatriclaben with three commercial fasciolicides in experimentally infected sheep. Parasitol Res 119, 2687–2693 (2020). https://doi.org/10.1007/s00436-020-06705-4
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DOI: https://doi.org/10.1007/s00436-020-06705-4