Skip to main content
SpringerLink
Log in

Discovery of a novel microsporidium in laboratory colonies of Mediterranean cricket Gryllus bimaculatus (Orthoptera: Gryllidae): Microsporidium grylli sp. nov.

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

A microsporidium was found in a Mediterranean cricket Gryllus bimaculatus from a pet market in the UK and a lab stock at the Moscow Zoo (originating from London Zoo). The spores were ovoid, uninucleate, 6.3 × 3.7 μm in size (unfixed), in packets by of 8, 16, or 32. The spores were easily discharged upon dessication or slight mechanical pressure. The polar tube was isofilar, with 15–16 coils arranged in 1–2 rows. The polaroplast was composed of thin lamellae and occupied about one third of the spore volume. The endospore was 200 nm thick, thinning over the anchoring disc. The exospore was thin, uniform, and with no ornamentation. Phylogenetics based upon small subunit ribosomal RNA (Genbank accession # MG663123) and RNA polymerase II largest subunit (# MG664544) genes placed the parasite at the base of the Trachipleistophora/Vavraia lineage. The RPB1 locus was polymorphic but similar genetic structure and identical clones were found in both isolates, confirming their common geographic origin. Due to in insufficient ultrastructural data and prominent divergence from described species, the parasite is provisionally placed to the collective taxon Microsporidium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Ayieko MA, Ogola HG, Ayieko IA (2016) Introducing rearing crickets (gryllids) at household levels: adoption, processing and nutritional values. J Insects Food Feed 2:203–211. https://doi.org/10.3920/JIFF2015.0080

    Article  Google Scholar 

  • Cheney SA, Lafranchi-Tristem NJ, Canning EU (2000) Phylogenetic relationships of Pleistophora-like microsporidia based on small subunit ribosomal DNA sequences and implications for the source of Trachipleistophora hominis infections. J Eukaryot Microbiol 47:280–287. https://doi.org/10.1111/j.1550-7408.2000.tb00048.x

    Article  PubMed  CAS  Google Scholar 

  • Cheney SA, Lafranchi-Tristem NJ, Bourges D, Canning EU (2001) Relationships of microsporidian genera, with emphasis on the polysporous genera, revealed by sequences of the largest subunit of RNA polymerase II (RPB1). J Eukaryot Microbiol 48:111–117

    Article  PubMed  CAS  Google Scholar 

  • Choudhary MM, Metcalfe MG, Arrambide K, Bern C, Visvesvara GS, Pieniazek NJ, Bandea RD, DeLeon-Carnes M, Adem P, Choudhary MM, Zaki SR, Saeed MU (2011) Tubulinosema sp. microsporidian myositis in immunosuppressed patient. Emerg Infect Dis 17:1727–1730. https://doi.org/10.3201/eid1709.101926

    Article  PubMed  PubMed Central  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hollister WS, Canning EU, Weidner E, Field AS, Kench J, Marriott DJ (1996) Development and ultrastructure of Trachipleistophora hominis n.g., n.sp. after in vitro isolation from an AIDS patient and inoculation into athymic mice. Parasitology 112:143–154

    Article  PubMed  Google Scholar 

  • Jones DT, Taylor WR, Thornton JM (1992) The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci 8:275–282

    PubMed  CAS  Google Scholar 

  • Just F, Essbauer S, Ahne W, Blahak S (2001) Occurrence of an invertebrate iridescent-like virus (Iridoviridae) in reptiles. J Veterinary Med Ser B 48:685–694

    Article  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2015) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054

    Article  CAS  Google Scholar 

  • Lorbacher de Ruiz H, Gelderblom H, Hofmann W, Darai G (1986) Insect iridescent virus type 6 induced toxic degenerative hepatitis in mice. Med Microbiol Immunol 175:43–53

    Article  PubMed  CAS  Google Scholar 

  • Meissner EG, Bennett JE, Qvarnstrom Y, da Silva A, Chu EY, Tsokos M, Gea-Banacloche J (2012) Disseminated microsporidiosis in an immunosuppressed patient. Emerg Infect Dis 18:1155–1158. https://doi.org/10.3201/eid1807.120047

    Article  PubMed  PubMed Central  Google Scholar 

  • Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  • Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671–675. https://doi.org/10.1038/nmeth.2089

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Silvestro D, Michalak I (2012) RaxmlGUI: a graphical front-end for RAxML. Org Divers Evol 12:335–337. https://doi.org/10.1007/s13127-011-0056-0

    Article  Google Scholar 

  • Sokolova YY, Dolgikh VV, Morzhina EV, Nassonova ES, Issi IV, Terry RS, Ironside JE, Smith JE, Vossbrinck CR (2003) Establishment of the new genus Paranosema based on the ultrastructure and molecular phylogeny of the type species Paranosema grylli Gen. Nov., Comb. Nov. (Sokolova, Selezniov, Dolgikh, Issi 1994), from the cricket Gryllus bimaculatus Deg. J Invertebr Pathol 84:159–172. https://doi.org/10.1016/j.jip.2003.10.004

    Article  PubMed  CAS  Google Scholar 

  • Sprague V, Becnel JJ, Hazard EI (1992) Taxonomy of phylum Microspora. Crit Rev Microbiol 18:285–395

    Article  PubMed  CAS  Google Scholar 

  • Vandeweyer D, Crauwels S, Lievens B, Van Campenhout L (2017) Metagenetic analysis of the bacterial communities of edible insects from diverse production cycles at industrial rearing companies. Int J Food Microbiol 261:11–18. https://doi.org/10.1016/j.ijfoodmicro.2017.08.018

    Article  PubMed  CAS  Google Scholar 

  • Vavra J, Kamler M, Modry D, Koudela B (2011) Opportunistic nature of the mammalian microsporidia: experimental transmission of Trachipleistophora extenrec (Fungi: microsporidia) between mammalian and insect hosts. Parasitol Res 108:1565–1573. https://doi.org/10.1007/s00436-010-2213-3

    Article  PubMed  Google Scholar 

  • Vogelstein B, Gillespie D (1979) Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A 76:615–619

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Vossbrinck CR, Debrunner-Vossbrinck BA (2005) Molecular phylogeny of the Microsporidia: ecological, ultrastructural and taxonomic considerations. Folia Parasitol 52:131–142. https://doi.org/10.14411/fp.2005.017

    Article  PubMed  CAS  Google Scholar 

  • Vossbrinck CR, Debrunner-Vossbrinck BA, Weiss LM (2014) Molecular phylogeny of the Microsporidia. In: Weiss LM, Becnel JJ (eds) Microsporidia: pathogens of opportunity. Wiley-Blackwell, pp 203–220

  • Weidner E, Canning EU, Rutledge CR, Meek CL (1999) Mosquito (Diptera: Culicidae) host compatibility and vector competency for the human myositic parasite Trachipleistophora hominis (Phylum Microspora). J Med Entomol 36:522–525

    Article  PubMed  CAS  Google Scholar 

  • Weiser J, Wegensteiner R, Zizka Z (1997) Ultrastructures of Nosema typographi Weiser 1955 (Microspora: Nosematidae) of the Bark Beetle Ips typographus L. (Coleoptera; Scolytidae). J Invertebr Pathol 70:156–160

    Article  PubMed  CAS  Google Scholar 

  • Weiss LH, Vossbrinck CR (1999) Molecular biology, molecular phylogeny, and molecular diagnostic approaches to the microsporidia. In: Wittner M, Weiss LM (eds) The microsporidia and microsporidiosis. ASM Press, Washington, pp 129–171

    Chapter  Google Scholar 

Download references

Funding

This study was funded by the Russian Science Foundation (grant # 16-14-00005).

Author information

Authors and Affiliations

  1. All-Russian Institute of Plant Protection, Podbelskogo 3, St. Petersburg, Pushkin, Russia, 196608

    Yuri S. Tokarev, Julia M. Malysh & Igor V. Senderskiy

  2. Aberystwyth University, Penglais, Aberystwyth, Ceredigion, SY23 3FL, Wales, UK

    Katy M. Peat

Authors
  1. Yuri S. Tokarev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Katy M. Peat
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Julia M. Malysh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Igor V. Senderskiy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Julia M. Malysh.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tokarev, Y.S., Peat, K.M., Malysh, J.M. et al. Discovery of a novel microsporidium in laboratory colonies of Mediterranean cricket Gryllus bimaculatus (Orthoptera: Gryllidae): Microsporidium grylli sp. nov.. Parasitol Res 117, 2823–2829 (2018). https://doi.org/10.1007/s00436-018-5970-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-018-5970-z

Keywords

Search

Navigation