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Excretory/secretory products from two Fasciola hepatica isolates induce different transcriptional changes and IL-10 release in LPS-activated bovine “BOMA” macrophages

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Abstract

Fasciola hepatica are trematodes that reside in the bile ducts of mammals. Infection causes US$3 billion in losses annually in animal production and is considered a zoonosis of growing importance. An under-represented area in F. hepatica research has been the examination of the different immunomodulatory abilities of various parasite isolates on the host immune system. In this paper, this issue was explored, with the bovine macrophage cell line “BOMA”. The cells were matured by LPS treatment and stimulated with excretory/secretory antigens (ES) from two Fasciola hepatica isolates: a laboratory isolate “Weybridge” (Fh-WeyES) and a wild isolate (Fh-WildES). As expected, stimulation with antigen mixtures with highly similar compositions resulted in mild transcriptomic differences. However, there were significant differences in cytokine levels. Compared to Fh-WeyES, exposure to Fh-WildES upregulated 27 and downregulated 30 genes. Fh-ES from both isolates diminished the release of TNF-α, whereas only Fh-WildES decreased IL-10 secretion. Neither Fh-WeyES nor Fh-WildES had an impact on IL-12 release. Our results indicate that various isolates can have different immunomodulatory abilities and impacts on the bovine immune system.

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Acknowledgments

This research was supported by a grant from the Polish National Science Centre (Project no. N N304 156340). We are grateful to the Scientific Council of Białowieża National Park which gave us permission to collect biological material from bison from Białowieża National Park.

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Authors and Affiliations

  1. Division of Pharmacology and Toxicology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, ul. Ciszewskiego 8, 02-786, Warsaw, Poland

    Piotr Bąska

  2. Witold Stefański Institute of Parasitology, Polish Academy of Sciences, ul. Twarda 51/55, 00-818, Warsaw, Poland

    Luke James Norbury, Anna Zawistowska-Deniziak & Kamil Januszkiewicz

  3. Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, ul. Ciszewskiego 8, 02-786, Warsaw, Poland

    Marcin Wiśniewski

  4. Central Forensic Laboratory of the Police – CFLP, Aleje Ujazdowskie 7, 00-583, Warsaw, Poland

    Kamil Januszkiewicz

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  1. Piotr Bąska
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  2. Luke James Norbury
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  3. Anna Zawistowska-Deniziak
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  5. Kamil Januszkiewicz
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Correspondence to Piotr Bąska.

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All experiments with animals followed institutional ethical guidelines and were approved by the Third Local Ethical Committee of Warsaw University of Life Sciences – SGGW (Permission no. 82/2010).

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Bąska, P., Norbury, L.J., Zawistowska-Deniziak, A. et al. Excretory/secretory products from two Fasciola hepatica isolates induce different transcriptional changes and IL-10 release in LPS-activated bovine “BOMA” macrophages. Parasitol Res 116, 2775–2782 (2017). https://doi.org/10.1007/s00436-017-5588-6

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