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Genetic characterization of human hydatid cysts shows coinfection by Echinococcus canadensis G7 and Echinococcus granulosus sensu stricto G1 in Argentina

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Abstract

Human cystic echinococcosis caused by the larval stage of Echinococcus granulosus sensu lato (s.l.) is a highly endemic disease in the province of Neuquén, Patagonia, Argentina. Human infections with E. granulosus sensu stricto (s.s.) G1 and Echinococcus canadensis G6 were reported in Neuquén in previous studies, whereas four genotypes were identified in livestock: G1, G3, G6, and G7. The aim of this study was to identify the genotypes of E. granulosus s.l. isolates from humans of Neuquén province, Patagonia, Argentina, through the 2005–2014 period. Twenty six hydatid cysts were obtained from 21 patients. The most frequent locations were the liver and lungs. Single cysts were observed in 81.0% of patients, and combined infection of liver and lungs was detected in 9.5% of cases. Partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes identified the presence of E. granulosus s.s. G1 (n = 11; 42.3%) including three different partial sequences; E. canadensis G6 (n = 14; 53.8%) and E. canadensis G7 (n = 1; 3.9%). Coinfection with G1 and G7 genotypes was detected in one patient who harbored three liver cysts. Most of the liver cysts corresponded to G1 and G6 genotypes. This study presents the first report in the Americas of a human infection with E. canadensis G7 and the second worldwide report of a coinfection with two different species and genotypes of E. granulosus s.l in humans. The molecular diversity of this parasite should be considered to redesign or improve the control program strategies in endemic regions.

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Acknowledgements

The authors are grateful with the surgery department of public and private hospitals of Neuquén province.

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Authors and Affiliations

  1. Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional del Comahue, Neuquén, Argentina

    María Florencia Debiaggi, Silvia Viviana Soriano, Nora Beatriz Pierangeli & Lorena Evelina Lazzarini

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Neuquén, Argentina

    María Florencia Debiaggi

  3. Laboratorio Central, Subsecretaría de Salud de Neuquén, Neuquén, Argentina

    Luis Alfredo Pianciola & Melina Leonor Mazzeo

  4. Cátedra de Anatomía e Imágenes Normales, Facultad de Ciencias Médicas, Universidad Nacional del Comahue, Neuquén, Argentina

    Sergio Moguillansky

  5. Centro Universitario de Estudios Microbiológicos y Parasitológicos (CUDEMyP) sede Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina

    Juan Angel Basualdo Farjat

Authors
  1. María Florencia Debiaggi
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  2. Silvia Viviana Soriano
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  3. Nora Beatriz Pierangeli
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  4. Lorena Evelina Lazzarini
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  5. Luis Alfredo Pianciola
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  6. Melina Leonor Mazzeo
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  7. Sergio Moguillansky
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  8. Juan Angel Basualdo Farjat
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Corresponding author

Correspondence to María Florencia Debiaggi.

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Funding

This study was funded by grant 04/N018 from the Secretaría de Ciencia y Técnica de la Universidad Nacional del Comahue.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This research study was approved by the Science and Technology Secretary of the National University of Comahue according to Permission No. 0991/05 Record No. 02327/05 dated on September 27, 2005. All adult subjects and parents/tutors of the children gave their written informed consent for surgery. The information of patients has been anonymised so that they cannot be identified.

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Debiaggi, M.F., Soriano, S.V., Pierangeli, N.B. et al. Genetic characterization of human hydatid cysts shows coinfection by Echinococcus canadensis G7 and Echinococcus granulosus sensu stricto G1 in Argentina. Parasitol Res 116, 2599–2604 (2017). https://doi.org/10.1007/s00436-017-5555-2

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  • DOI: https://doi.org/10.1007/s00436-017-5555-2

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