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Comparative studies on the proteomic expression patterns in the third- and fifth-stage larvae of Angiostrongylus cantonensis

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Abstract

Angiostrongylus cantonensis is an important zoonotic parasite causing eosinophilic meningitis and eosinophilic meningoencephalitis in humans. In this study, the protein expression profiles of the infective third- and pathogenic fifth-stage larvae (L3 and L5) of this parasite were compared by proteomic techniques. Isolated protein samples were separated by two-dimensional gel electrophoresis (2-DE), stained with silver nitrate, and analyzed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Proteins from L5 were mainly at pH 5–7 and with molecular weight (MW) 40–100 kDa, whereas those from L3 were at pH 5–6 and with 5–35 kDa. Of 100 protein spots identified, 33 were from L3 whereas 67 from L5 and 63 had known identities, whereas 37 were hypothetical proteins. There were 15 spots of stress proteins, and HSP60 was the most frequently found heat stress proteins in L5. More binding and protein transport-related proteins were found in L5 including peptidylprolyl isomerase (cyclophilin)-like 2, serum albumin, preproalbumin precursor, and dilute class unconventional myosin. L3 had a higher expression of cytoskeleton and membrane proteins than L5. In addition, four protein spots were identified in the sera of the rat host by Western blot analysis. The present proteomic study revealed different protein expression profiles in L3 and L5 of A. cantonensis. These changes may reflect the development of L3 from the poikilothermic snails to L5 in the homoeothemic rats. This information may be useful for the finding of stage-specific proteins and biomarker for diagnosis of angiostrongyliasis.

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Acknowledgments

This work was supported in part by grants from the National Science Council, Executive Yuan, ROC (NSC100-2320-B-182-013 and NSC101-2320-B-182-045) and Chang Gung Memorial Hospital Research Grant CMRPD1B0351. We thank Chang Gung Molecular Medicine Research Center for technical supports.

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Authors and Affiliations

  1. Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 333, Taiwan

    Kuang-Yao Chen, Chien-Ju Cheng, Petrus Tang & Lian-Chen Wang

  2. Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan, 333, Taiwan

    Petrus Tang & Lian-Chen Wang

  3. Department of Parasitology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan

    Chuan-Min Yen

  4. Bioinformatics Center, Chang Gung University, Kwei-Shan, Tao-Yuan, 333, Taiwan

    Petrus Tang

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  1. Kuang-Yao Chen
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  2. Chien-Ju Cheng
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  3. Chuan-Min Yen
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  4. Petrus Tang
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  5. Lian-Chen Wang
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Correspondence to Lian-Chen Wang.

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Chen, KY., Cheng, CJ., Yen, CM. et al. Comparative studies on the proteomic expression patterns in the third- and fifth-stage larvae of Angiostrongylus cantonensis . Parasitol Res 113, 3591–3600 (2014). https://doi.org/10.1007/s00436-014-4024-4

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  • DOI: https://doi.org/10.1007/s00436-014-4024-4

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