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Impact of Argemone mexicana extracts on the cidal, morphological, and behavioral response of dengue vector, Aedes aegypti L. (Diptera: Culicidae)

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Abstract

The larvicidal, behavioral, and morphological response of dengue vector, Aedes aegypti treated with deleterious weed, Argemone mexicana, was explored. The 1,000 ppm extracts of A. mexicana leaf, stem, and roots prepared in five different solvents (petroleum ether, hexane, benzene, acetone, and ethanol) were screened for their larvicidal activity against dengue vector establishing the efficacy of petroleum ether and hexane extracts. Other extracts, unable to give 100 % mortality, were considered ineffective and discarded from further study. Larvicidal bioassay conducted with selected extracts confirmed the higher efficacy of hexane extracts exhibiting 1.1- to 1.8-fold more potential than the petroleum ether extracts. The results further revealed 1.6- to 2.4-fold higher efficacy of root extracts than those prepared from the leaves and stem of A. mexicana. The hexane root extract of A. mexicana was found to be the most effective larvicide with LC50 value of 91.331 ppm after 24 h of exposure causing 1.8 and 2.4 fold more toxicity as compared to the hexane leaf and stem extracts, respectively. Prolonged exposure of the larvae to the extracts resulted in increased toxicity potential of the extracts. Observations of the treated larvae revealed excitation, violent vertical, and horizontal movements with aggressive anal biting behavior suggesting effect of extracts on their neuromuscular system. Morphological studies of the treated larvae revealed the demelanization of cuticle and shrinkage of internal cuticle of anal papillae indicating the anal papillae as the probable action sites of the A. mexicana extracts. The potential of A. mexicana as new larvicides against dengue vector are being explored.

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Acknowledgments

The authors are highly grateful to University Grants Commission, New Delhi for providing research fellowship to carry out the present investigations. Thanks are extended to Dr. Savithri Singh, Principal, Acharya Narendra Dev College for providing infrastructure and research facilities.

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  1. Department of Zoology, Acharya Narendra Dev College, University of Delhi, New Delhi, India

    Radhika Warikoo & Sarita Kumar

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  1. Radhika Warikoo
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  2. Sarita Kumar
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Correspondence to Radhika Warikoo.

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Warikoo, R., Kumar, S. Impact of Argemone mexicana extracts on the cidal, morphological, and behavioral response of dengue vector, Aedes aegypti L. (Diptera: Culicidae). Parasitol Res 112, 3477–3484 (2013). https://doi.org/10.1007/s00436-013-3528-7

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  • DOI: https://doi.org/10.1007/s00436-013-3528-7

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