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The first determination of Trichuris sp. from roe deer by amplification and sequenation of the ITS1-5.8S-ITS2 segment of ribosomal DNA

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Abstract

Trichuris nematodes were isolated from roe deer (Capreolus capreolus). At first, nematodes were determined using morphological and biometrical methods. Subsequently genomic DNA was isolated and the ITS1-5.8S-ITS2 segment from ribosomal DNA (RNA) was amplified and sequenced using PCR techniques. With u sing morphological and biometrical methods, female nematodes were identified as Trichuris globulosa, and the only male was identified as Trichuris ovis. The females were classified into four morphotypes. However, analysis of the internal transcribed spacers (ITS1-5.8S-ITS2) of specimens did not confirm this classification. Moreover, the female individuals morphologically determined as T. globulosa were molecularly identified as Trichuris discolor. In the case of the only male molecular analysis match the result of the molecular identification. Furthermore, a comparative phylogenetic study was carried out with the ITS1 and ITS2 sequences of the Trichuris species from various hosts. A comparison of biometric information from T. discolor individuals from this study was also conducted.

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References

  • Allen RW (1955) Parasites of mountain sheep in New Mexico, with new host records. J Parasitol 41:583–587

    Article  PubMed  CAS  Google Scholar 

  • Aukštikalniené R, Bukelskis E, Kašetaité E (2007) Intestinal helminthes of Cervidae in the Aukštaitija National Park. Balt For 13:96–102

    Google Scholar 

  • Balicka-Ramisz A, Cisek A, Ramisz A, Pilarczyk B (2003) Investigation of the lung, stomach and intestine helminth infections of roe deer in North-West Poland. Tierarztl Umsch 58:489–491

    Google Scholar 

  • Barth D, Dollinger P (1975) Zur Wirtsspezifität der Magen-Darm-Nematoden von Reh, Schaf und Rind. Z Jagdwiss 21:164–182

    Google Scholar 

  • Baruš V, Kotrlá B, Tenora F (1977) A scanning electron microscopic study of spicular sheath of some trichurids (Nematoda). Folia Parasitol 24:107–110

    Google Scholar 

  • Baruš V, Kotrlá B, Tenora F (1978) Scanning elektron microscopic study of the vulva of some trichurids (Nematoda). Folia Parasitol 25:31–34

    PubMed  Google Scholar 

  • Blood DA (1963) Parasites from California bighorn sheep in southern British Columbia. Can J Zool 41:913–918

    Article  Google Scholar 

  • Callejón R, de Rojas M, Ariza C, Ubeda JM, Guevara DC, Cutillas C (2008) Cytochrome oxidase subunit 1 and mitochondrial 16S rDNA sequences of Trichuris skrjabini (Tricocephalida: Trichuridae). Parasitol Res 104:715–716

    Article  PubMed  Google Scholar 

  • Callejón R, Halajian A, de Rojas M, Marrugal A, Guevara DC, Cutillas C (2012) 16S partial gene mitochondrial DNA and internal transcribed spacers ribosomal DNA as differential markers of Trichuris discolor populations. Vet Parasitol 186:350–363

    Article  PubMed  Google Scholar 

  • Chroust K, Vitula F (2005) Anthelmintická účinnost Cermixu premix na hlístice u zvěře. Veterinarstvi 55:707–713

    Google Scholar 

  • Cordero del Campillo M, Rojo Vázquez FA (1999) Parasicología Veterinaria. McGraw-Hill, Interamericana, Madrid

    Google Scholar 

  • Cutillas C, German P, Arias P, Guevara D (1995) Trichuris ovis and Trichuris globulosa: morphological, biometrical, and genetic studies. Exp Parasitol 81:621–625

    Article  PubMed  CAS  Google Scholar 

  • Cutillas C, German P, Arias P, Guevera D (1996) Characterization of Trichuris skrjabini by isoenzyme gel electrophoresis: comparative study with Trichuris ovis. Acta Trop 62:63–69

    Article  PubMed  CAS  Google Scholar 

  • Cutillas C, Oliveros R, de Rojas M, Guevara DC (2004) Determination of Trichuris skrjabini by sequencing of the ITS1–5.8S – ITS2 segment of the ribosomal DNA: comparative molecular study of different species of trichurids. J Parasitol 90:648–652

    Article  PubMed  CAS  Google Scholar 

  • Dróždž J, Demiaszkiewicz A, Lachowicz J (1992) The helminth fauna of the roe deer Capreolus capreolus (L.) in a hunting area inhibited by red deer, elk and European bison (Borecka Forest, Poland) over the yearly cycle. Acta Parasitol 37:83–88

    Google Scholar 

  • Gagarin VG (1972) Analiz vidogo sostava trihicefalov zvačnyh, zaregistrirovannyh v Sovetskom Spuze. Trudy Vses. Inst Gelmintol im´KI Skrjabina 19:39–75

    Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hinks MI, Thomas RJ (1974) A new record of the occurrence of Trichuris skrjabini Baskakov, 1924 in sheep in Britain. J Helmintol 48:33–38

    Article  CAS  Google Scholar 

  • Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  PubMed  CAS  Google Scholar 

  • Itagaki T, Ohtori M, Aoki M, Ichinomiya M (2007) Molecular phylogenetic analysis of gastrointestinal helminth from japanese serow (Capricornis crispus) in North region. Published only in dataset

  • Kaur H, Duggal CL (2007) Studies on the male and female copulatory apparatus of Trichuris globulosa (Nematoda, Trichuridae). Helmintologia 44:151–156

    Article  Google Scholar 

  • Knight RA (1971) Redescription of Trichuris discolor (von Linstow, 1906) and T. skrjabini (Baskakov, 1924) from domestic ruminants in the United States and comparasions with T. ovis (Abildgaard, 1795). J Parasitol 57:302–310

    Article  PubMed  CAS  Google Scholar 

  • Knight RA (1984) Morphological differences in Trichuris ovis associated with different host species. J Parasitol 70:842–843

    Article  PubMed  CAS  Google Scholar 

  • Kuzmina TA, Kharchenko VA, Malega AM (2010) Helminth fauna of Roe deer (Capreolus capreolus) in Ukraine: biodiversity and Parasite community. Vestn Zool 44:12–19

    Google Scholar 

  • Lanfredi RM, De Souza W, Gomes DC (1995) Comparative study of four species of Trichuris Roederer, 1761 (Nematoda, Trichurinae) by scanning electron microscopy. Mem Inst Oswaldo Cruz 90:489–496

    Article  Google Scholar 

  • Noda R (1955) Trichuris species from giraffe and cattle. Bull Univ Osaka 5:119–126

    Google Scholar 

  • Oliveros R, Cutillas C, de Rojas M, Arias P (2000) Characterazition of four species of Trichuris (Nematoda: Enoplida) by their second internal transcribed spacer ribosomal DNA sequence. Parasitol Res 86:1008–1013

    Article  PubMed  CAS  Google Scholar 

  • Ortlepp RJ (1937) Whipworms from Soııth African Ruminants. Onderstepoort. J Vet Scien Anim Ind 9:91–100

    Google Scholar 

  • Pedreira J, Silva AP, Andrade RS, Suarez JL, Arias M, Lomba C, Diaz P, Lopez C, Banos PD, Morrondo P (2006) Prevalence of gastrointestinal parasites in sheep and parasite control practices in North - West Spain. Prev Vet Med 75:56–62

    Article  PubMed  CAS  Google Scholar 

  • Rehbein S, Kollmannsberger M, Visser M, Winter R (1996) Untersuchungen zum HelminthenbefaI1 von Schlachtschafen in Oberbayern. 1. Mitt Berl Mfinch Tieriirztl Wschr 109:161–167

    CAS  Google Scholar 

  • Rehbein S, Lutz W, Visser M, Winter R (2000) Beitrage zur Kenntnis der Parasitenfauna des Wildes in Nordrhein-Westfalen. 1. Der Endoparasitenbefall des Rehwildes. Z Jagdwiss 46:248–269

    Google Scholar 

  • Rehbein S, Lutz W, Visser M, Winter R (2001) Beitrage zur Kenntnis der Parasitenfauna des Wildes in Nordrhein-Westfalen. 2. Der Endoparasitenbefall des Damwildes. Z Jagdwiss 47:1–16

    Google Scholar 

  • Rehbein S, Lutz W, Visser M, Winter R (2002) Beitrage zur Kenntnis der Parasitenfauna des Wildes in Nordrhein-Westfalen. 3. Der Endoparasitenbefall des Rotwildes. Z Jagdwiss 48:69–93

    Google Scholar 

  • Rickard LG, Bishop JK (1991) Redescription of Trichuris tenuis Chandler, 1930, from llamas (Lama glama) in Oregon with a key to the species of Trichuris present in North American ruminants. J Parasitol 71:70–75

    Article  Google Scholar 

  • Sarwar MM (1945) Two new records of the species of the genus Trichuris from indian ruminants. Curr Sci 5:156

    Google Scholar 

  • Sarwar MM (1957) A critical survey of the representation of the genus Trichuris in Ruminants in Indo-Pakistan. Acta Trop 14:225–227

    PubMed  CAS  Google Scholar 

  • Sarwar MM (1959) Reconstruction of the genus Trichuris and a short review of its taxonomy and morphology. Biologia (Lahore) 5:19–35

    Google Scholar 

  • Shimalov VV, Shimalov VT (2003) Helminth fauna of cervids in Belorussian Polesie. Parasitol Res 89:75–76

    Article  PubMed  CAS  Google Scholar 

  • Skrjabin KI, Shikhobalova NP, Orlow IV (1957) Trichocephalidae and Capillariidae of animals and the man and the diseases caused by them. Acad Sci, USSR

  • Špakulová M (1994) Discriminant analysis as a method for the numerical evaluation of taxonomic characters in male trichurid nematodes. Syst Parasitol 29:113–119

    Article  Google Scholar 

  • Swofford DL (2000) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland

    Google Scholar 

  • Tenora F, Ooi HK, Staněk M, Kamiya M (1992) Some novel features of male posterior end of Trichuris discolor as revealed by scanning electron microscopy. Jpn J Parasitol 41:487–491

    Google Scholar 

  • Umur S, Yukari BA (2005) An abatoir survey of gastro-intestinalnematodes in sheep in the Burdur region. Turk J Vet Anim Sci 29:1195–1201

    Google Scholar 

  • Vázquez L, Dacal V, Pato FJ, Paz-Silva A, Dijez-Baňos N, López C, Panadero R, Sánchez R, Dijez-Baňos P, Morrondo P (2009) The occurence of endoparasites of roedeer (Capreolus capreolus) in two differences areas from new Spain. Rew Ibero–Latino Am Parasitol 1:25–31

    Google Scholar 

  • Wright KA (1978) Structure and function of male copulatory apparatus of the nematodes Capillaria hepatice and Trichuris muris. Can J Zool 56:651–662

    Article  PubMed  CAS  Google Scholar 

  • Zhu X, Gasser RB, Jacobs DE, Hung GC, Chilton NB (2000) Relationships among some ascaridoid nematodes based on ribosomal DNA sequence data. Parasitol Res 86:738–744

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This study has been funded by the Grant of the National Agency for Agricultural Research of the Czech Republic, v.v.i. No. QI111A199. We would also like to thank Mr. Brian Kavalir for his proofreading services.

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Authors and Affiliations

  1. Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague 6-Suchdol, Czech Republic

    O. Salaba, K. Rylková, J. Vadlejch, M. Petrtýl, Š. Scháňková, A. Brožová, I. Jankovská & I. Langrová

  2. Department of Husbandry and Ethology of Animals, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague 6-Suchdol, Czech Republic

    L. Jebavý

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  1. O. Salaba
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  2. K. Rylková
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  3. J. Vadlejch
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  4. M. Petrtýl
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Correspondence to I. Langrová.

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Salaba, O., Rylková, K., Vadlejch, J. et al. The first determination of Trichuris sp. from roe deer by amplification and sequenation of the ITS1-5.8S-ITS2 segment of ribosomal DNA. Parasitol Res 112, 955–960 (2013). https://doi.org/10.1007/s00436-012-3215-0

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  • DOI: https://doi.org/10.1007/s00436-012-3215-0

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