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Investigation on the 19S ATPase proteasome subunits (Rpt1–6) conservation and their differential gene expression in Schistosoma mansoni

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Abstract

The ubiquitin-proteasome system is responsible for degradation of the majority of intracellular proteins in eukaryotic cells. The 26S proteasome proteolytic complex is composed of a 20S core particle responsible for protein degradation and the 19S lid which plays a role in the recognition of polyubiquitinated substrates. The 19S regulatory particle (Rps) is composed of ATPase (Rpt) and non-ATPase (Rpn) subunits. In this study, we analyzed the expression profile of 19S Rpt subunits in the larvae and adult stage of the Schistosoma mansoni life cycle. Conventional reverse transcriptase polymerase chain reaction (RT-PCR) revealed that the majority of the 19S Rpt subunits amplified at the expected molecular masses for various investigated stages. In addition, SmRpt1, SmRpt2, and SmRpt6 transcript levels were increased in 3 h-cultured schistosomula and reasonably maintained until 5 h in culture, as revealed by qRT-PCR. Phylogenetic analysis of 19S Rpt subunits showed high structural conservation in comparison to other Rpt orthologues. The mRNA expression profile of 19S Rpt subunits did not correlate with 26S proteasome proteolytic activity as judged by a 14C-casein-degrading assay, in the early cultured schistosomula. Taken together, these results revealed a differential expression profile for 19S Rpt subunits whose transcript levels could not be directly associated to 26S proteasome activity.

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Acknowledgments

This work was supported by grants from Conselho Nacional de Pesquisa e Desenvolvimento, Fundação de Amparo a Pesquisa do Estado de São Paulo, and Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior. The authors are grateful to Elenice Macedo and Olinda Mara Brigato for their technical assistance.

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Authors and Affiliations

  1. Centro de Ciências Agrárias-CCA/UFES, Departamento de Zootecnia, Alto Universitário, s/n Centro, 29500-000, Alegre, Espírito Santo, Brazil

    Olavo S. Pereira-Júnior

  2. Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av Bandeirantes 3900, Monte Alegre, Ribeirão Preto, São Paulo, Brazil

    Camila S. Silva, Fernanda J. Cabral, Sérgio H. Silva, Cláudia S. Soares, Enyara R. Morais, Érika B. C. Moreira & Vanderlei Rodrigues

  3. Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, Campus Universitário, Morro do Cruzeiro, 35400-000, Ouro Preto, Minas Gerais, Brazil

    Roberta Verciano Pereira, William Castro-Borges & Renata Guerra Sá

  4. Hospital das Clínicas da Faculdade de Medicina da, Universidade de São Paulo, Av Dr Enéas Carvalho de Aguiar, 500, Prédio Instituto Medicina TropicaI Il, 2 andar. Cerqueira Cesar, 05403-000, São Paulo, São Paulo, Brazil

    Fabiana M. de Paula

  5. Laboratório de Pesquisa em Parasitologia, Universidade de Franca, Av. Dr. Armando Salles de Oliveira Parque Universitário, 4404-600, Franca, São Paulo, Brazil

    Lizandra G. Magalhães

Authors
  1. Olavo S. Pereira-Júnior
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  2. Roberta Verciano Pereira
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  3. Camila S. Silva
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  4. William Castro-Borges
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  5. Renata Guerra Sá
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  6. Fernanda J. Cabral
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  7. Sérgio H. Silva
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  8. Cláudia S. Soares
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  9. Enyara R. Morais
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  10. Érika B. C. Moreira
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  11. Lizandra G. Magalhães
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  12. Fabiana M. de Paula
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  13. Vanderlei Rodrigues
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Corresponding author

Correspondence to Vanderlei Rodrigues.

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Pereira-Júnior, O.S., Pereira, R.V., Silva, C.S. et al. Investigation on the 19S ATPase proteasome subunits (Rpt1–6) conservation and their differential gene expression in Schistosoma mansoni . Parasitol Res 112, 235–242 (2013). https://doi.org/10.1007/s00436-012-3130-4

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  • DOI: https://doi.org/10.1007/s00436-012-3130-4

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