Abstract
The aims of this study were to investigate the prevalence of Cryptosporidium and to characterize the genotype distribution of Cryptosporidium isolates in native beef calves 2–6 months old in Dac Lac province, central Vietnam. The presence of Cryptosporidium oocysts was determined using the modified Ziehl–Neelsen staining method. The overall prevalence on the sample and herd levels were 18.9 % (44/232) and 50 % (20/40), respectively. Genotyping based on PCR and sequence analysis of the 18 S rRNA gene revealed occurrence of the two nonzoonotic species Cryptosporidium ryanae and Cryptosporidium bovis, with the former as a dominant species in the animals. The absence of the zoonotic species Cryptosporidium parvum in calves examined suggests that the native beef calves 2–6 months old in the study area are unlikely to contribute to human cryptosporidiosis transmission.
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Acknowledgments
This study was supported by the Japan Society for the Promotion of Science (JSPS) under Ronpaku Fellowship (No VAST-10936) for Sam Thi Nguyen. The authors would like to thank colleagues in the Laboratory of Sustainable Environmental Biology, Tohoku University and at the Central Vietnam Veterinary Institute for their support.
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Nguyen, S.T., Fukuda, Y., Tada, C. et al. Molecular characterization of Cryptosporidium in native beef calves in central Vietnam. Parasitol Res 111, 1817–1820 (2012). https://doi.org/10.1007/s00436-012-3038-z
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DOI: https://doi.org/10.1007/s00436-012-3038-z