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Inhibitory effect of Ferula asafoetida L. (Umbelliferae) on Blastocystis sp. subtype 3 growth in vitro

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Abstract

Asafoetida is an oleo-gum-resin obtained from many Ferula species and frequently used in traditional medicine. The current study aimed to evaluate the activity of asafoetida against the in vitro growth of Blastocystis sp. Asafoetida as powder-form (Ap) and oil-form (Ao) extracts at concentrations of 2, 4, 8, 16, and 20 mg/ml and 5, 10, 25, 40, and 50 mg/ml, respectively were incubated with isolates of Blastocystis sp. subtype 3 for 24, 72, and 144 h and compared to the reference antiprotozoan drug metronidazole at concentrations of 10, 100, and 500 μg/ml. Asafoetida either as Ap or Ao decreased counts and viability of all tested isolates of Blastocystis sp. subtype 3 which was confirmed by microscopy. The degree of the inhibitory effect was dependent on the concentration, form, as well as the time of incubation with asafoetida extracts. The lowest concentrations of Ap and Ao that caused complete (100 %) inhibition of Blastocystis growth and highest (100 %) percentage inhibition of multiplication was 16 and 40 mg/ml, respectively; mean counts at these concentrations either did not differ or decreased significantly when compared to metronidazole control (p < 0.05). Also, the parasites did not resume growth after re-cultivation in asafoetida-free medium when examined further after 48, 72, and 144 h of cultivation. These findings demonstrate the potential of phytomedicine asafoetida as a potent natural alternative for treatment of Blastocystis sp.infection.

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References

  • Abd El-Razek MH (2007) A new ester isolated from Ferula assa-foetida L. Biosci Biotechnol Biochem 71(9):2300–2303

    Article  PubMed  CAS  Google Scholar 

  • Abd El-Razek MH, Ohta S, Ahmed AA, Hirata T (2001) Sesquiterpene coumarins from the roots of Ferula assa-foetida. Phytochem 58:1289–1295

    Article  CAS  Google Scholar 

  • Abdel Hameed DM, Hassanin OM (2011) Proteaese activity of Blastocystis hominis subtype3 in symptomatic and asymptomatic patients. Parasitol Res 109:321–327

    Article  PubMed  Google Scholar 

  • Abdel Hameed DM, Hassanin OM, Zuel-Fakkar NM (2011) Association of Blastocystis hominis genetic subtypes with urticaria. Parasitol Res 108:553–560

    Article  Google Scholar 

  • Afifi MM, El-Wakil HS, Abdel-Ghaffar MM (2000) A novel chemotherapeutic combination for Trichomonas vaginalis targeting purine salvage pathways of the parasite. J Egypt Soc Parasitol 24(3):611–619

    Google Scholar 

  • Arribas JM, Fernandez GH, Escalera GI, Pardilla AI, Martin TA, Gonzalez De La Rosa JB (2001) Acute infectious lymphocytosis associated to Giardia lamblia and Blastocystis hominis coinfection. An Esp Pediatr 54:518–520

    PubMed  CAS  Google Scholar 

  • Borchardt KA, Li Z, Zhang MZ, Shing H (1996) An in vitro metronidazole susceptibility test for trichomoniasis using the InPouch TV test. Genitourin Med 72:132–135

    PubMed  CAS  Google Scholar 

  • Calzada F, Yepez-Mulia L, Tapia-Contreras A (2007) Effect of Mexican medicinal plant used to treat trichomoniasis on Trichomonas vaginalis trophozoites. J Ethnopharmacol 113:248–251

    Article  PubMed  Google Scholar 

  • Chinsembu KC, Hedimbi M (2010) An ethnobotanical survey of plants used to manage HIV/AIDS opportunistic infections in Katima Mulilo, Caprivi region. Namibia J Ethnobiol Ethnomed 6:25

    Article  Google Scholar 

  • Dehpour AA, Ebrahimzadeh MA, Fazel NS, Mohammad NS (2009) Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition. Grasas y Aceites 60:405–412

    Article  CAS  Google Scholar 

  • Dinleyici EC, Eren M, Dogan N, Reyhanioglu S, Yargic ZA, Vandenplas Y (2011) Clinical efficacy of Saccharomyces boulardii or metronidazole in symptomatic children with Blastocystis hominis infection. Parasitol Res 108:541–545

    Article  PubMed  Google Scholar 

  • Ebrahimzadeh MA, Nabavi SF, Nabavi SM, Pourmorad F (2010) Nitric oxide radical scavenging potential of some Elburz medicinal plants. Afr J Biotech 9(32):5212–5217

    CAS  Google Scholar 

  • Egyptian pharmacopoeia (1984) 3rd edn. General Organization for Government Printing Offices, Cairo

  • El Wakil SS (2007) Evaluation of the in vitro effect of Nigella sativa aqueous extract on Blastocystis hominis isolates. J Egypt Soc Parasitol 37:801–813

    PubMed  Google Scholar 

  • Force M, Sparks WS, Ronzio RA (2000) Inhibition of enteric parasites by emulsified oil of oregano in vivo. Phytother Res 14:213–214

    Article  PubMed  CAS  Google Scholar 

  • Frasson AP, Santos O, Duarte M, Trentin DS, Giordani RB, Silva AG, Silva MV, Tasca T, Macedo AJ (2011) First report of anti-Trichomonas vaginalis activity of the medicinal plant Polygala decumbens from the Brazilian semi-arid region, Caatinga. Parasitol Res. DOI 10.1007/s00436-011-2787-4

  • Garcia LS (2001) Macroscopic and microscopic examination of fecal specimens. In: Diagnostic Medical Parasitology, 4th edn. The American Society of Microbiology, Washington, D.C, pp 741–785

    Google Scholar 

  • González-Coloma A, Reina M, Sáenz C, Lacret R, Ruiz-Mesia L, Arán VJ, Sanz J, Martínez-Díaz RA (2011) Antileishmanial, antitrypanosomal, and cytotoxic screening of ethnopharmacologically selected Peruvian plants. Parasitol Res. DOI 10.1007/s00436-011-2638-3

  • Grabensteiner E, Arshad N, Hess M (2007) Differences in the in vitro susceptibility of mono-eukaryotic cultures of Histomonas meleagridis, Tetratrichomonas gallinarum and Blastocystis sp. to natural organic compounds. Parasitol Res 101:193–199

    Article  PubMed  Google Scholar 

  • Grabensteiner E, Liebhart D, Arshad N, Hess M (2008) Antiprotozoal activities determined in vitro and in vivo of certain plant extracts against Histomonas meleagridis, Tetratrichomonas gallinarum and Blastocystis sp. Parasitol Res 103:1257–1264

    Article  PubMed  Google Scholar 

  • Haresh K, Suresh K, Khairul A, Saminathan S (1999) Isolate resistance of Blastocystis hominis to metronidazole. Trop Med Int Hlth 4:274–277

    Article  CAS  Google Scholar 

  • Hussein EM, Hussein AM, Eida MM, Atwa MM (2008) Pathophysiological variability of different genotypes of human Blastocystis hominis Egyptian isolates in experimentally infected rats. Parasitol Res 102:853–860

    Article  PubMed  Google Scholar 

  • Iranshahy M, Iranshahy M (2011) Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assa-foetida oleo-gum-resin)–a review. J Ethnopharmacol 134(1):1–10

    Article  PubMed  CAS  Google Scholar 

  • Jackson D, Rakwar J, Bwayo J, Kreiss J, Moses S (1997) Urethral Trichomonas vaginalis infection and HIV-1 transmission. Lancet 350:1076–1077

    Article  PubMed  CAS  Google Scholar 

  • Johnson PJ (1993) Metronidazole and drug resistance. Parasitol Today 9:183–186

    Article  PubMed  CAS  Google Scholar 

  • Jones W (1946) The experimental infection of rats with Entamoeba histolytica. Ann Trop Med Parasitol 40:130–138

    PubMed  CAS  Google Scholar 

  • Kaneda Y, Horiki N, Cheng XJ, Tachibana H, Tsutsumi Y (2000) Serologic response to Blastocystis hominis infection in asymptomatic individuals. Tokai J Exp Clin Med 25:51–56

    PubMed  CAS  Google Scholar 

  • Kaneda Y, Horiki N, Cheng X, Fujita Y, Maruyama M, Tachibana H (2001) Ribodeme of Blastocystis hominis isolated in Japan. Am J Trop Med Hyg 65(4):393–396

    PubMed  CAS  Google Scholar 

  • Kapoor LD (1990) Handbook of Ayurvedic medicinal plants. CRC, Boca Raton, p 185

    Google Scholar 

  • Keshri GV, Lakshmi V, Singh MM, Kamboj VP (1999) Post-coital antifertility activity of Ferula assafoetida extract in female rats. Pharm Biol 37(4):273–276

    Article  Google Scholar 

  • Koné WM, Vargas M, Keiser J (2011) Anthelmintic activity of medicinal plants used in Côte d’Ivoire for treating parasitic diseases. Parasitol Res. DOI 10.1007/s00436-011-2771-z

  • Kumar P, Singh DK (2006) Molluscicidal activity of Ferula asafoetida, Syzygium aromaticum and Carum carvi and their active components against the snail Lymnaea acuminata. Chemosphere 63:1568–1574

    Article  PubMed  CAS  Google Scholar 

  • Lee CL, Chiang LC, Cheng LH, Liaw CC, Abd El-Razek MH, Chang FR, Wu YC (2009) Influenza A (H1N1) antiviral and cytotoxic agents from Ferula assa-foetida. J Nat Prod 72:1568–1572

    Article  PubMed  CAS  Google Scholar 

  • Leelayoova S, Rangsin R, Taamasri P, Naaglor T, Thathaisong U, Mungthin M (2004) Evidence of waterborne transmission of Blastocystis hominis. Am J Trop Med Hyg 70(6):658–662

    PubMed  Google Scholar 

  • Lemee V, Zaharia I, Nevez G, Rabodonirina M, Brasseur P, Ballet JJ, Favennec L (2000) Metronidazole and albendazole susceptibility of 11 clinical isolates of Giardia duodenalis from France. J Antimicrob Chemother 46:819–821

    Article  PubMed  CAS  Google Scholar 

  • Mallikarjuna GU, Dhanalakshmi S, Raisuddin S, Rao AR (2003) Chemomodulatory influence of Ferula asafoetida on mammary epithelial differentiation, hepatic drug metabolizing enzymes, antioxidant profiles and N-methyl-N-nitrosourea-induced mammary carcinogenesis in rats. Breast Cancer Res Treat 81:1–10

    Article  PubMed  CAS  Google Scholar 

  • Meri T, Jokiranta ST, Suhonen L, Meri S (2000) Resistance of Trichomonas vaginalis to metronidazole: report of the first three cases from Finland and optimization of the in vitro susceptibility testing under various oxygen concentrations. J Clin Microbiol 38:763–767

    PubMed  CAS  Google Scholar 

  • Mishra N, Behal KK (2010) Antimicrobial activity of some spices against selected microbes. Int J Pharmacy and Pharm Sci 2(3):187–196

    Google Scholar 

  • Noël C, Dufernez F, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Ho LC, Singh M, Wintjens R, Sogin ML, Capron M, Pierce R, Zenner L, Viscogliosi E (2005) Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. J Clin Microbiol 43:348–355

    Article  PubMed  Google Scholar 

  • Özyurt M, Kurt O, Molbak K, Nielsen HV, Haznedaroglu T, Stensvold CR (2008) Molecular epidemiology of Blastocystis infections in Turkey. Parasitol Int 57:300–306

    Article  PubMed  Google Scholar 

  • Ramadan NI, Al Khadrawy FM (2003) The in vitro effect of Assafoetida on Trichomonas vaginalis. J Egypt Soc Parasitol 33(2):615–630

    PubMed  Google Scholar 

  • Ramadan NI, Abdeel Aaty HE, Abdel-Hameed DM, El Deeb HK, Mansy SA, Al Khadrawy FM, Samir NA (2004) Effect of Assafoetida on adult Schistosoma mansoni in experimentally infected mice. J Egypt Soc Parasitol 34(3):1077–1094

    PubMed  Google Scholar 

  • Rollinger JM, Steindl TM, Schuster D, Kirchmair J, Anrain K, Ellmerer EP, Langer T, Stuppner H, Wutzler P, Schmidtke M (2008) Structure-based virtual screening for the discovery of natural inhibitors for human rhinovirus coat protein. J Med Chem 51:842–851

    Article  PubMed  CAS  Google Scholar 

  • Saleem M, Alam A, Sultana S (2001) Asafoetida inhibits early events of cacinogenesis: a chemopreventive study. Life Sci 68(16):1913–1921

    Article  PubMed  CAS  Google Scholar 

  • Sá-Nunes A, Faccioli LH, Sforcin JM (2003) Propolis: lymphocyte proliferation and IFN-gamma production. J Ethnopharmacol 87(1):93–97

    Article  PubMed  Google Scholar 

  • Sawangjaroen N, Sawangjaroen K (2005) The effects of extracts from anti-diarrheic Thai medicinal plants on the in vitro growth of the intestinal protozoa parasite: Blastocystis hominis. J Ethnopharmacol 98:67–72

    Article  PubMed  Google Scholar 

  • Souppart L, Sanciu G, Cian A, Wawrzyniak I, Delbac F, Capron M, Dei-Cas E, Boorom K, Delhaes L, Viscogliosi E (2009) Molecular epidemiology of human Blastocystis isolates in France. Parasitol Res 105(2):413–421

    Article  PubMed  Google Scholar 

  • Souppart L, Moussa H, Cian A, Sanciu G, Poirier P, El Alaoui H, Delbac F, Boorom K, Delhaes L, Dei-Cas E, Viscogliosi E (2010) Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt. Parasitol Res 106:505–511

    Article  PubMed  Google Scholar 

  • Stenzel DJ, Boreham PF (1996) Blastocystis hominis revisited. Clin Microbiol Rev 9:563–584

    PubMed  CAS  Google Scholar 

  • Suryawanshi SN, Pandey S, Rashmirathi, Bhatt BA, Gupta S (2007) Chemotherapy of leishmaniasis Part VI: synthesis and bioevaluation of some novel terpenyl S,N- and N,N-acetals. Eur J Med Chem 42:511–516

    Article  PubMed  CAS  Google Scholar 

  • Takeoka G (2001) Volatile constituents of Asafoetida. In: Takeoka GR, Guntert M, Engel KH (eds) Aroma active compounds in foods. American Chemical society, Washington, DC, pp 33–44

    Chapter  Google Scholar 

  • Tan KSW (2008) New insights on classification, identification and clinical relevance of Blastocystis spp. Clin Microbiol Rev 21:639–665

    Article  PubMed  CAS  Google Scholar 

  • Tan TC, Suresh KG, Smith HV (2008) Phenotypic and genotypic characterisation of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. Parasitol Res 104:85–93

    Article  PubMed  CAS  Google Scholar 

  • Vital PG, Rivera WL (2009) Antimicrobial activity and cytotoxicity of Chromolaena odorata (L. f.) King and Robinson and Uncaria perrottetii (A. Rich) Merr. Extracts. J Med Plants Res 3(7):511–518

    Google Scholar 

  • Wallis TE (1999) Textbook of pharmacognosy, 5th edn. Churchill Livingstone.

  • Yakoob J, Jafri W, Jafri N, Khan R, Islam M, Beg MA, Zaman V (2004) Irritable bowel syndrome: in search of an etiology: role of Blastocystis hominis. Am J Trop Med Hyg 70(4):383–385

    PubMed  Google Scholar 

  • Yakoob J, Jafri W, Beg MA, Abbas Z, Naz S, Islam M, Khan R (2010) Blastocystis hominis and Dientamoeba fragilis in patients fulfilling irritable bowel syndrome criteria. Parasitol Res 107:679–684

    Article  PubMed  Google Scholar 

  • Yakoob J, Abbas Z, Beg MA, Naz S, Safia Awan S, Hamid S, Jafri W (2011) In vitro sensitivity of Blastocystis hominis to garlic, ginger, white cumin, and black pepper used in diet. Parasitol Res 109:379–385

    Article  PubMed  Google Scholar 

  • Yan Y, Su S, Lai R, Liao H, Ye J, Li X, Luo X, Chen G (2006) Genetic variability of Blastocystis hominis isolates in China. Parasitol Res 99:597–601

    Article  PubMed  Google Scholar 

  • Yang LQ, Singh M, Yap EH, Ng GC, Xu HX, Sim KY (1996) In vitro response of Blastocystis hominis against traditional Chinese medicine. J Ethnopharmacol 55:35–42

    Article  PubMed  CAS  Google Scholar 

  • Yoshikawa H, Wu Z, Kimata I, Iseki M, Ali IK, Hossain MB, Zaman V, Haque R, Takahashi Y (2004) Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries. Parasitol Res 92:22–29

    Article  PubMed  Google Scholar 

  • Yoshikawa H, Dogruman-Al F, Turk S, Kustimur S, Balaban N, Sultan N (2011) Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples. Parasitol Res 109:1045–1050

    Article  PubMed  Google Scholar 

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Acknowledgments

We are grateful to the Research and Diagnostic Lab unit of the Parasitology Department, Faculty of Medicine, Ain Shams University, Egypt for offering free use of all the facilities while carrying out this study. We would like to thank Prof. Heba Abdel Aaty and Prof. Nashwa Ramadan for their scientific consulting and kind help. We herewith declare that the execution of the research described in this article complied with the current laws and regulations of the Egyptian Ministry of Higher Education and Scientific Research.

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Medicine, Ain Shams University, P.O box 16 Saray El Kobba, 11712, Cairo, Egypt

    Hala K. El Deeb

  2. Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt

    Faisal M. Al Khadrawy

  3. Department of Biochemistry, Faculty of Medicine, Menoufia University, Shebin El kom, Egypt

    Ayman K. Abd El-Hameid

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  1. Hala K. El Deeb
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  2. Faisal M. Al Khadrawy
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Correspondence to Hala K. El Deeb.

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El Deeb, H.K., Al Khadrawy, F.M. & El-Hameid, A.K.A. Inhibitory effect of Ferula asafoetida L. (Umbelliferae) on Blastocystis sp. subtype 3 growth in vitro. Parasitol Res 111, 1213–1221 (2012). https://doi.org/10.1007/s00436-012-2955-1

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