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Molecular cloning and expression of the VK247 circumsporozoite protein for serodiagnosis of variant form Plasmodium vivax

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Abstract

Plasmodium vivax is classified into two serotypes, VK210 [the dominant form- GDRA(D/A)GQPA repeats] and VK247 [the variant form-ANGA(G/D)(N/D)QPG repeats], based on sequence variation of the repeat region of the circumsporozoite (CS) protein gene. Genomic DNA for the variant CS protein gene was obtained from field isolate strains in Myanmar. The repetitive region has highly 19 immunogenic repeats flanked by non-repeat stretches of amino acids. The sequence including this region (717 bp) was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein has a molecular weight of about 50 kDa as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Anti-VK247 antibodies were found in malaria patients who have been exposed to variant form of P. vivax in western blot analysis. Therefore, this recombinant protein might be a useful tool in serodiagnosis of malaria patients who have been infected with variant form of P. vivax.

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Acknowledgements

This work was supported by an internal grant from Korea National Institute of Health, Republic of Korea.

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Authors and Affiliations

  1. Department of Parasitology, College of Medicine, Inha University, Incheon, 405-751, South Korea

    Tong-Soo Kim

  2. Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, Seoul, 122-701, South Korea

    Tong-Soo Kim, Sun-Sim Lee, Chang-Mi Oh, Kyung-Mi Choi, Jung-Yeon Kim & Hyeong-Woo Lee

  3. Vascular Medicine Research Unit, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, 02139, USA

    Hyung-Hwan Kim

  4. International Research Center for Bioscience and Biotechnology, Jungwon University, Goesan, 367-805, South Korea

    Hyung-Hwan Kim & Hyuck Kim

  5. Department of Medical Research (Upper Myanmar), Pying Oo Lwin Township, Mandalay, Myanmar

    Khin Lin

  6. Department of Parasitology and Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju, 660-751, South Korea

    Byoung-Kuk Na

  7. Department of Parasitology, Kangwon National University, College of Medicine, Chuncheon, 200-701, South Korea

    Eun-Taek Han

  8. Department of Gynecology, College of Oriental Medicine, Sangji University, Wonju, 220-717, South Korea

    Youngjoo Sohn

  9. Department of Pathology, University of Florida, J-566, 1600 SW Archer Road, Gainesville, FL, 32610, USA

    Hyeong-Woo Lee

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  1. Tong-Soo Kim
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  2. Hyung-Hwan Kim
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  3. Sun-Sim Lee
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  6. Khin Lin
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  9. Eun-Taek Han
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  10. Youngjoo Sohn
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  11. Hyuck Kim
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  12. Hyeong-Woo Lee
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Corresponding author

Correspondence to Hyeong-Woo Lee.

Additional information

Tong-Soo Kim, Hyung-Hwan Kim and Sun-Sim Lee contributed equally to this work.

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Kim, TS., Kim, HH., Lee, SS. et al. Molecular cloning and expression of the VK247 circumsporozoite protein for serodiagnosis of variant form Plasmodium vivax . Parasitol Res 108, 1275–1282 (2011). https://doi.org/10.1007/s00436-010-2177-3

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  • DOI: https://doi.org/10.1007/s00436-010-2177-3

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