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Excretory/secretory proteome of the adult stage of Echinostoma caproni

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Abstract

The excretory/secretory proteome of Echinostoma caproni (Trematoda: Echinostomatidae) adults collected from experimentally infected mice was investigated using a proteomic approach. We performed a shot-gun liquid chromatography/tandem mass spectrometry for the separation and identification of tryptic peptides from the excretory/secretory products of E. caproni adult worms. Database search was performed using MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems). A total of 39 parasite proteins were accurately identified. Strikingly, metabolic enzymes, and particularly glycolytic enzymes, constituted the largest protein family in the excretory/secretory proteome of E. caproni adult worms. Moreover, representative proteins involved in parasite structure, response against stress, chaperones, calcium-binding, and signal transduction were also identified. This work extends our knowledge of host–parasite relationships in the E. caproni-rodent model that is extensively used to analyze the factors determining the intestinal helminth rejection. Consequently, information on many proteins may be useful to better understand the molecular basis that determines the survival of this parasite in the definitive host.

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Acknowledgements

This work was supported by the projects CGL2005-0231/BOS from the Ministerio de Ciencia e Innovación and FEDER (European Union), PROMETEO/2009/081 from Conselleria d'Educació, Generalitat Valenciana (Valencia, Spain), PS09/02355 from the Fondo de Investigación Sanitaria (FIS) del Ministerio de Ciencia e Innovación (Madrid, Spain) and FEDER, and UV-AE-10-23739 from the Universitat de València (Valencia, Spain). This work has been carried out while the first author (J.S.) was recipient of a pre-doctoral fellowship from the Ministerio de Ciencia e Innovación (Madrid, Spain). This research complies with the current laws for animal health research in Spain.

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Authors and Affiliations

  1. Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100, Burjassot, Valencia, Spain

    Javier Sotillo, J. Guillermo Esteban, Antonio Marcilla & Rafael Toledo

  2. Laboratorio de Proteómica, Centro de Investigación Principe Felipe, Av. Autopista del Saler 16, 46013, Valencia, Spain

    M. Luz Valero & Manuel M. Sánchez Del Pino

  3. Department of Biology, Lafayette College, Easton, PA, 18042, USA

    Bernard Fried

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  1. Javier Sotillo
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  2. M. Luz Valero
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  3. Manuel M. Sánchez Del Pino
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  4. Bernard Fried
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  5. J. Guillermo Esteban
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  6. Antonio Marcilla
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  7. Rafael Toledo
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Correspondence to Rafael Toledo.

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Sotillo, J., Valero, M.L., Sánchez Del Pino, M.M. et al. Excretory/secretory proteome of the adult stage of Echinostoma caproni . Parasitol Res 107, 691–697 (2010). https://doi.org/10.1007/s00436-010-1923-x

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  • DOI: https://doi.org/10.1007/s00436-010-1923-x

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