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In vitro schistosomicidal effects of some phloroglucinol derivatives from Dryopteris species against Schistosoma mansoni adult worms

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Abstract

The rhizomes of Dryopteris species have popularly been used as vermifuge in flatworm infections. The aim of this work was to evaluate the in vitro schistosomicidal activity of some phloroglucinol compounds, obtained from the rhizomes of Dryopteris species, against Schistosoma mansoni adult worms. All worm pairs were dead after 24 h of incubation with aspidin 25 to 100 μM (1), flavaspidic acid 50 and 100 μM (2), methylene-bis-aspidinol 100 μM (3), and desaspidin 25 to 100 μM (4). Worms incubated with 1 (25 to 100 μM) and 2 (50 to 100 μM) showed decrease motor activity with tegumental alterations, while 3 (100 μM) and 4 (10 to 100 μM) showed decrease motor activity without tegumental alterations. Desaspidinol (5) and filicinic acid (6), at the tested concentrations (10 to 100 μM), did not show activity against adult worms of S. mansoni. Praziquantel (10 μM), used as positive control, caused death of the parasites and tegumental alterations without separation of worms. In the groups treated with 100 µM of compounds 1–4, the viability of the adult worms was similar to the positive control group, in which the worms were dead. Also, both the egg productions and the development of eggs produced by the adult worms were inhibited by the incubation with compounds 1–4 (10 and 100 μM) in comparison with the negative control (RPMI 1640 medium). It is suggested that the in vitro schistosomicidal effects of phloroglucinols derivatives 1, 2, 3, and 4 may be related to the inhibition of oxidative phosphorylation pathway in S. mansoni. The present results confirmed the traditional indications of rhizomes from Dryopteris species, which possess phloroglucinol compounds, in the treatment of tapeworm infections.

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Acknowledgements

The authors are grateful to FAPESP (Grant nos. 2009/05701-1; 2009/05478-0) and CNPq/PIBIC/Unifran (Grant no. 100059/2009-0) for scholarships and FAPESP for financial support (Grant no. 2006/60132-4). The authors are grateful to the late Dr. Aneri Pentilla, Helsinki, Finland, for providing the phloroglucinols used in this study. Sincere appreciation is also extended to Professor C. J. Widen, Department of Pharmacognosy, University of Helsinki, Finland, for his interest in this work. We are also grateful to Elenice A. Macedo and Olinda Mara Brigatto for their excellent technical assistance.

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Authors and Affiliations

  1. Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900, Ribeirão Preto, SP, Brazil

    Lizandra G. Magalhães & Vanderlei Rodrigues

  2. Department of Pharmaceutical Sciences, School of Pharmacy, Howard University, 2300 4th Street, NW, Washington, DC, 20059, USA

    Govind J. Kapadia

  3. Grupo de Pesquisas em Produtos Naturais, Núcleo de Pesquisas em Ciências Exatas e Tecnológicas da Universidade de Franca, 14404-600, Franca, SP, Brazil

    Lígia R. da Silva Tonuci, Soraya C. Caixeta, Natállia A. Parreira & Ademar A. Da Silva Filho

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  1. Lizandra G. Magalhães
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  2. Govind J. Kapadia
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  3. Lígia R. da Silva Tonuci
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  4. Soraya C. Caixeta
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  5. Natállia A. Parreira
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  6. Vanderlei Rodrigues
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  7. Ademar A. Da Silva Filho
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Correspondence to Ademar A. Da Silva Filho.

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Magalhães, L.G., Kapadia, G.J., da Silva Tonuci, L.R. et al. In vitro schistosomicidal effects of some phloroglucinol derivatives from Dryopteris species against Schistosoma mansoni adult worms. Parasitol Res 106, 395–401 (2010). https://doi.org/10.1007/s00436-009-1674-8

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