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Sarcocystis in the birds family Corvidae with description of Sarcocystis cornixi sp. nov. from the hooded crow (Corvus cornix)

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Abstract

Having studied 67 birds of six species of the family Corvidae, Sarcocystis cysts were found in 16 (23.9%) individuals belonging to three species. The highest prevalence of infection (35.9%) was determined in the hooded crow (Corvus cornix). Two types of sarcocysts, which were temporarily called cysts type I and type V, were determined in the corvids examined. By light microscope, type I cyst wall seemed to be thin (< 1.0 μm) and smooth. Banana shaped cystozoites measured 6.0–8.0 μm in length. By light microscope, type V cyst wall seemed striated and reached up to 2.5 μm. Banana shaped cystozoites measured 6.1–7.9 × 1.4–1.8 μm. Ultrastructurally, the cyst wall amounted to 2.1 μm and had stump-like protrusions that differed greatly in size and shape. The parasitophorous vacuolar membrane had indentations and clearly visible (up to 0.2 μm in length) microprojections, which also differed considerably in size and shape. The ultrastructure of type V cyst wall differed from all those Sarcocystis spp. described thus far. On the basis of this—Sarcocystis cornixi sp. nov.—is proposed for this type of sarcocysts. Partial sequences of 18S rRNA and 28S rRNA genes were determined for this species and a phylogenetic analysis of the Sarcocystidae family was performed. In the phylogenetic tree, S. cornixi is grouped together with Frenkelia microti, F. glareoli, S. muris, S. neurona and the unnamed Sarcocystis species whose intermediate hosts are birds. S. cornixi is the most closely related to Sarcocystis sp. (cyst type I) from the white-fronted geese.

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References

  • Aljanabi SM, Martinez I (1997) Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res 25:4692–4693

    Article  CAS  PubMed  Google Scholar 

  • Černá Ž (1984) The role of birds as definitive hosts and intermediate hosts of heteroxenous coccidians. J Protozool 31:579–581

    PubMed  Google Scholar 

  • Dahlgren SS, Gjerde B (2007) Genetic characterisation of six Sarcocystis species from reindeer (Rangifer tarandus tarandus) in Norway based on the small subunit rRNA gene. Vet Parasitol 146:204–213

    CAS  PubMed  Google Scholar 

  • Dahlgren SS, Gjerde B (2008) Sarcocystis in moose (Alces alces): molecular identification and phylogeny of six Sarcocystis species in moose, and a morphological description of three new species. Parasitol Res 103:93–110

    Article  PubMed  Google Scholar 

  • Dahlgren SS, Gjerde B, Skirnisson K, Gudmundsdottir B (2007) Morphological and molecular identification of three species of Sarcocystis in reindeer (Rangifer tarandus tarandus) in Iceland. Vet Parasitol 149:191–198

    Article  PubMed  Google Scholar 

  • Doležel D, Koudela B, Jirků M, Hypša V, Oborník M, Votýpka J, Modrý D, Šlapeta JR, Lukeš J (1999) Phylogenetic analysis of Sarcocystis spp. of mammals and reptiles supports the coevolution of Sarcocystis spp. with their final hosts. Int J Parasitol 29:795–798

    Article  PubMed  Google Scholar 

  • Drouin TE, Mahrt JL (1979) The prevalence of Sarcocystis Lankester, 1882, in some bird species in western Canada, with notes on its life cycle. Can J Zool 57:1915–1921

    Article  CAS  PubMed  Google Scholar 

  • Dubey JP, Speer CA, Fayer R (1989) Sarcocystosis of animals and man. CRC, Boca Raton, FL

    Google Scholar 

  • Dylko NI (1962) Distribution of Sarcosporidia among wild vertebrates in Belorussia. Dokl Akad Nauk 6:399–400

    Google Scholar 

  • Elsheikha HM, Lacher DW, Mansfield LS (2005) Phylogenetic relationships of Sarcocystis neurona of horses and opossums to other cyst-forming coccidia deduced from SSU rRNA gene sequences. Parasitol Res 97:345–357

    Article  PubMed  Google Scholar 

  • Jeffries AC, Schnitzler B, Heydorn AO, Johnson AM, Tenter AM (1997) Identification of synapomorphic characters in the genus Sarcocystis based on 18S rDNA sequences comparison. J Eukaryot Microbiol 44:388–392

    Article  CAS  PubMed  Google Scholar 

  • Krone O, Rudolph M, Jakob W (2000) Protozoa in the breast muscle of raptors in Germany. Acta Protozool 39:35–42

    Google Scholar 

  • Kutkienė L, Sruoga A (2004) Sarcocystis spp. in birds of the order Anseriformes. Parasitol Res 92:171–172

    Article  PubMed  Google Scholar 

  • Kutkienė L, Sruoga A, Butkauskas D (2006) Sarcocystis sp. from white-fronted goose (Anser albiforns): cyst morphology and life cycle studies. Parasitol Res 99:562–565

    Article  PubMed  Google Scholar 

  • Lindsay DS, Blagburn BL (1999) Prevalence of encysted apicomplexans in muscles of raptors. Vet Parasitol 80:341–344

    Article  CAS  PubMed  Google Scholar 

  • Logminas V (comp.) (1990) Fauna of Lithuania. Aves 1. Mokslas, Vilnius

  • Mehlhorn H, Heydorn AO (1978) The Sarcosporidia (Protozoa, Sporozoa): life cycle and fine structure. Adv Parasitol 16:43–91

    Article  CAS  PubMed  Google Scholar 

  • Mugridge NB, Morrison DA, Johnson AM, Luton K, Dubey JP, Votýpka J, Tenter AM (1999) Phylogenetic relationships of the genus Frenkelia: a review of its history and new knowledge gained from comparison of large subunit ribosomal ribonucleic acid gene sequences. Int J Parasitol 29:957–972

    Article  CAS  PubMed  Google Scholar 

  • Mugridge NB, Morrison DA, Jäkel T, Heckeroth AR, Tenter AM, Johnson AM (2000) Effects of sequence alignment and structural domains of ribosomal DNA on phylogeny reconstruction for the protozoan family Sarcocystidae. Mol Biol Evol 17:1842–1853

    CAS  PubMed  Google Scholar 

  • Munday BL, Hartley WJ, Harrigan KE, Presidente PJA, Obendorf DL (1979) Sarcocystis and related organisms in Australian wildlife: II. Survey findings in birds, reptiles, amphibians and fish. J Wildl Dis 15:57–73

    CAS  PubMed  Google Scholar 

  • Odening K, Stolte M, Walter G, Bockhardt I (1994) The European badger (Carnivora: Mustelidae) as intermediate host of further three Sarcocystis species (Sporozoa). Parasite 1:23–30

    CAS  PubMed  Google Scholar 

  • Pak SM, Eshtokina NV (1984) Sarcosporidians of birds. In: Panin VJ (ed) Sarcosporidians of animals in Kazakhstan. Nauka, Alma-Ata, pp 150–168

    Google Scholar 

  • Šlapeta JR, Modrý D, Votýpka J, Jirků M, Koudela B, Lukeš J (2001) Multiple origin of the dihomoxenous life cycle in Sarcosporidia. Int J Parasitol 31:413–417

    Article  PubMed  Google Scholar 

  • Šlapeta JR, Modrý D, Votýpka J, Jirků M, Lukeš J, Koudela B (2003) Evolutionary relationships among cyst-forming coccidia Sarcocystis spp. (Alveolata: Apicomplexa: Coccidea) in endemic African tree vipers and perspective for evolution of heteroxenous life cycle. Mol Phylogenet Evol 27:464–475

    Article  PubMed  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Tenter AM, Baverstock PR, Johnson AM (1992) Phylogenetic relationships of Sarcocystis species from sheep, goats, cattle and mice based on ribosomal RNA sequences. Int J Parasitol 22:503–513

    Article  CAS  PubMed  Google Scholar 

  • Valkiūnas G, Križanauskienė A, Iezhova TA, Hellgren O, Bensch S (2007) Molecular phylogenetic analysis of circumnuclear haemoproteids (Haemosporida, Haemoproteidae) of sylviid birds, with description of Haemoproteus parabelopolskyi sp. nov. J Parasitol 93:680–687

    Article  PubMed  Google Scholar 

  • Votýpka J, Hypša V, Jirků M, Flegr J, Vávra J, Lukeš J (1998) Molecular pyhlogenetic relatedness of Frenkelia spp. (Protozoa, Apicomplexa) to Sarcocystis falcatula Stiles 1893: is the genus Sarcocystis paraphyletic. J Eukaryot Microbiol 45(1):137–141

    Article  PubMed  Google Scholar 

  • Wesemeier H-H, Sedlaczek J (1995) One known Sarcocystis species and two found for the first time in red deer and wapiti (Cervus elaphus) in Europe. Appl Parasitol 36:245–251

    CAS  PubMed  Google Scholar 

  • Yang ZQ, Zuo YX, Ding B, Chen XW, Luo J, Zhang YP (2001) Identification of Sarcocystis hominis-like (Protozoa: Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences. J Parasitol 87:934–937

    CAS  PubMed  Google Scholar 

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Acknowledgements

The authors are thankful to Dr. Jitender P. Dubey from Animal Parasitic Diseases Laboratory, United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute for evaluation of sarcocyst wall ultra-structure. We express our sincere gratitude to Professor Ilan Paperna from Hebrew University of Jerusalem for the valuable comments on the draft of the paper. We are grateful to Mrs. I. Žalakevičienė from the Institute of Experimental and Clinical Medicine for her help in carrying out investigations of electron microscopy.

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  1. Laboratory of Molecular Ecology, Institute of Ecology of Vilnius University, Vilnius, Lithuania

    Liuda Kutkienė, Petras Prakas, Aniolas Sruoga & Dalius Butkauskas

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  1. Liuda Kutkienė
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  2. Petras Prakas
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  3. Aniolas Sruoga
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  4. Dalius Butkauskas
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Correspondence to Petras Prakas.

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Kutkienė, L., Prakas, P., Sruoga, A. et al. Sarcocystis in the birds family Corvidae with description of Sarcocystis cornixi sp. nov. from the hooded crow (Corvus cornix). Parasitol Res 104, 329–336 (2009). https://doi.org/10.1007/s00436-008-1196-9

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  • DOI: https://doi.org/10.1007/s00436-008-1196-9

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