Abstract
We examined the eggshell structure of the blowfly, Chrysomya nigripes Aubertin, a species of forensic importance, using scanning electron microscopy. A relatively wide plastron region was located dorsally. It extended almost the entire length of the egg and bifurcated slightly, close to the micropyle, which is a deep cavity surrounded by an elevated, fine, wavy rim. The chorionic sculpture had an hexagonal pattern, with a smooth and elevated boundary. The ruptured eggshell showed the outermost exochorion, membranous outer endochorion and pillar layer, which also had an hexagonal pattern on a perforated mesh pillar layer. This study provides a greater database on the egg of this forensically significant blowfly.
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References
Bohart GE, Gressitt JL (1951) Filth-inhabiting flies of Guam. Bull Bernice P. Bishop Mus 204:1–152
Colwell DD, Baird CR, Lee B, Milton K (1999) Scanning electron microscopy and comparative morphometrics of eggs from six bot fly species (Diptera: Oestridae). J Med Entomol 36:803–810
Erzinclioğlu YZ (1989) The value of chorionic structure and size in the diagnosis of blowfly eggs. Med Vet Entomol 3:281–285
Greenberg B, Kunich JC (2002) Entomology and the law. Flies as forensic indicators, Cambridge University Press, Cambridge
Greenberg B, Singh D (1995) Species identification of calliphorid (Diptera) eggs. J Med Entomol 32:21–26
Greenberg B, Szyska ML (1984) Immature stages and biology of fifteen species of Peruvian Calliphoridae (Diptera). Ann Entomol Soc Am 77:488–517
Haskell NH (1990) Procedures in the entomology laboratory. In: Catts EP, Haskell NH (eds) Entomology and death: a procedural guide. Joyce’s Print Shop, Clemson, pp 111–123
Hinton HE (1960) Plastron respiration in the eggs of blowflies. J Insect Physiol 4:176–183
Jenson LM, Miller RH (2001) Estimating filth fly (Diptera: Calliphoridae) development in carrion in Guam. Micronesia 34:11–25
Kitching RL (1976) The immature stages of the Old-World screw-worm fly, Chrysomya bezziana Villeneuve, with comparative notes on other Australasian species of Chrysomya. Bull Entomol Res 66:195–203
Kurahashi H, Afzal M (2002) The blow flies recorded from Pakistan, with the description of one new species (Diptera: Calliphoridae). Med Entomol Zool 53 [Suppl 2]:213–230
Liu D, Greenberg B (1989) Immature stage of some flies of forensic importance. Ann Entomol Soc Am 82:80–93
Margaritis LH (1985) Structure and physiology of the eggshell. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry and pharmacology, vol I. Embryogenesis and reproduction. Pergamon Press, Oxford, pp 153–230
Peterson RD, Newman SMJr (1991) Chorionic structure of the egg of the screwworm Cochliomyia hominivorax (Diptera: Calliphoridae). J Med Entomol 28:152–160
Acknowledgement
This work was supported by the Faculty of Medicine Endowment Fund for Medical Research, Faculty of Medicine, Chiang Mai University.
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Sukontason, K., Sukontason, K.L., Boonchu, N. et al. Ultrastructure of eggshell of Chrysomya nigripes Aubertin (Diptera: Calliphoridae). Parasitol Res 93, 151–154 (2004). https://doi.org/10.1007/s00436-004-1102-z
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DOI: https://doi.org/10.1007/s00436-004-1102-z