Abstract
We studied the pattern of cGMP immunostaining (IS) after stimulation with a nitric oxide donor in the presence of an inhibitor of phosphodiesterase in adult Hymenolepis diminuta. cGMP-IS was detected in the peripheral nervous system, especially in nerve fibres close to the body muscle fibres. cGMP-IS also occurred in terminals beneath the basal lamina of the tegument and between the muscle fibres of the suckers. The pattern of cGMP-IS was compared to that of 5-HT-IS and GYIRFamide-IS. TRITC-conjugated phalloidin was used to stain the musculature.
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Acknowledgements
This investigation was supported by INTAS grant 00-0685, the Russian Foundation for Fundamental Research, the Research Institute of the Foundation of the Åbo Akademi University, the Finnish Academy of Science, Magnus Ehrnrooths Foundation and the Foundation for Swedish Culture in Finland. We wish to thank Dr. Jan de Vente, University of Limburg, Maastricht, The Netherlands for the antibodies to cGMP, and Mr. Esa Nummelin for technical assistance.
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Gustafsson, M.K.S., Terenina, N.B., Reuter, M. et al. NO nerves and their targets in a tapeworm: an immunocytochemical study of cGMP in Hymenolepis diminuta . Parasitol Res 90, 148–152 (2003). https://doi.org/10.1007/s00436-003-0826-5
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DOI: https://doi.org/10.1007/s00436-003-0826-5