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A potential probe set of fluorescence in situ hybridization for detection of lung cancer in bronchial brushing specimens

  • Original Paper
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Abstract

Purpose

The study aims to find candidate probes of fluorescence in situ hybridization (FISH) for detection of lung cancer with bronchial brushings and to evaluate whether the accuracy of diagnosing lung cancer by cytological deviant and genetic abnormalities is greater than that of cytology alone.

Methods

Centromeric enumeration probes (CEPs) for chromosomes 2, 3, 6, 7, 8, 9, 11, 12, and 17 were analyzed using FISH in 74 surgical resection tissues, 32 operative margin tissues without tumor involvement of lung cancer, and 174 bronchial brushings.

Results

The aneuploidy rates of the tested probes were 61.7, 89.1, 80.0, 92.7, 65.0, 70.4, 66.7, 71.8, 68.9 % in tumor tissues, and 29.3, 58.9, 33.3, 69.6, 67.0, 40.3, 38.0, 49.3, 35.1 % in bronchial brushings. The combination of cytology and FISH using the three-probe set for chromosomes 3+7+8 significantly improved the sensitivity of bronchial brushing examination for lung cancer detection (P = 0.00003), especially squamous cell carcinoma (SCC), which increased from 78.0 to 98.2 %. The specificity of the 3+7+8 probe set was 94.6 %. Moreover, a high aneuploidy rate of the probe set in bronchial brushings was detected more often in SCCs (P = 0.029) and late-stage non-small-cell lung cancer (NSCLC) (P = 0.044). Kaplan–Meier curves indicated that adenocarcinoma (ADC) patients with high aneuploidy rate of CEP3 in tissue samples exhibited poorer overall survival (P = 0.016).

Conclusions

FISH performed on cytology preparations is useful for confirmation of cancer diagnosis. The three-probe set, 3+7+8, has potential value for the detection of SCCs in bronchial brushings.

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Abbreviations

ADC:

Adenocarcinoma

CEP:

Centromeric enumeration probes

FISH:

Fluorescence in situ hybridization

NSCLC:

Non-small-cell lung cancer

OS:

Overall survival

SCC:

Squamous cell carcinoma

SCLC:

Small cell lung cancer

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Acknowledgments

This work was supported by Special Public Health Fund of China (200902002-4) and Chinese Hi-Tech R&D Program Grant (2012AA02A502 and 2011YQ17006710).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Molecular Oncology, Cancer Institute/Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, China

    Yi-Zhen Liu, Li-Li Fang, Lu Li, Jian Cao, Xin Xu, Ya-Ling Han, Yan Cai & Ming-Rong Wang

  2. Department of Critical Care Medicine, Yijishan Hospital, Wannan Medical College, Wuhu, 241001, China

    Zhen Wang

  3. Department of Cardio-Thoracic Surgery, Shanghai Tenth Hospital affiliated to Tongji University, Shanghai, 200072, China

    Liang-Xu Wang

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  1. Yi-Zhen Liu
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  2. Zhen Wang
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  3. Li-Li Fang
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  4. Lu Li
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  10. Ming-Rong Wang
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Correspondence to Ming-Rong Wang.

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Yi-Zhen Liu and Zhen Wang have contributed equally to this work.

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Liu, YZ., Wang, Z., Fang, LL. et al. A potential probe set of fluorescence in situ hybridization for detection of lung cancer in bronchial brushing specimens. J Cancer Res Clin Oncol 138, 1541–1549 (2012). https://doi.org/10.1007/s00432-012-1232-0

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  • DOI: https://doi.org/10.1007/s00432-012-1232-0

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