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Cellular localisation of Survivin: impact on the prognosis in colorectal cancer

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Abstract

Purpose: The present study was designed to determine whether the nuclear or cytoplasmic expression of survivin, was related to clinicopathological parameters and survival in sporadic colon carcinomas. Methods: Western blotting of cell fractions and immunocytochemical methodology were used in five human colon cancer cell lines. Immunohistochemical study was performed in formalin-fixed paraffin-embedded section from 46 patients with sporadic colorectal adenocarcinomas with a polyclonal antibody directed against survivin. Apoptotic index was evaluated by using the M30 antibody. Survival rates were estimated by the Kaplan–Meier method and compared using the log-rank test. Multivariate survival analysis was performed by the Cox proportional hazards model. Results: Western blotting and immunocytochemistry analyses confirmed that survivin could be detected both in the nucleus and the cytoplasm. Immunohistochemical analysis demonstrated that 39% of tumours expressed survivin in the nucleus and 41% in the cytoplasm. No relationship was observed between survivin expression and clinicopathological features. Unexpectedly, the apoptotic index appeared to be linked with high survivin nuclear expression. Overall, 3-year observed survival rate was 73% in patients with cytoplasmic survivin expression versus 48% for negative expression (P=0.14). Survival was 72% versus 50% for positive nuclear survivin expression versus negative (P=0.16). After adjustment for age and stage, cytoplasmic survivin expression was a significant prognostic factor. A high level of expression was associated to a better survival: RR=0.35 [0.13–0.98], P=0.045. Conclusion: These results indicate that the analysis of the subcellular expression of survivin is a determining factor to define the prognostic value. Its evaluation, using a polyclonal antibody, might help clinicians in the stratification of patients with colorectal cancer.

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Acknowledgements

This work was supported by grants of the “Ligue contre le cancer de Saône et Loire ” and “Fondation pour la recherche médicale”. We would like to thank David Rageot, Martine Goiset and Didier Carnet for technical help.

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Authors and Affiliations

  1. Faculté de Médicine, Service d’anatomie et de Cytologie Pathologiques, 7, boulevard Jeanne d’Arc, BP 87900, 21079, Dijon Cedex, France

    T. Ponnelle, C. Chapusot, L. Martin & F. Piard

  2. Registre Bourguignon des Cancers Digestifs, INSERM EMI 01-06, 21079, Dijon Cedex, France

    T. Ponnelle, C. Chapusot, A. M. Bouvier, J. Faivre & F. Piard

  3. Faculty of Medicine, INSERM U-517, IFR 100, 21079, Dijon Cedex, France

    S. Plenchette & E. Solary

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  1. T. Ponnelle
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  2. C. Chapusot
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  3. L. Martin
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Correspondence to T. Ponnelle.

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Ponnelle, T., Chapusot, C., Martin, L. et al. Cellular localisation of Survivin: impact on the prognosis in colorectal cancer. J Cancer Res Clin Oncol 131, 504–510 (2005). https://doi.org/10.1007/s00432-005-0682-z

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  • DOI: https://doi.org/10.1007/s00432-005-0682-z

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