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Human papillomavirus (HPV) types 16 and 18 in liquid-based cervical cytology samples

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Abstract

Human papillomavirus (HPV) DNA testing is replacing cervical cytology as a primary cervical cancer screening tool. The aim of this study was to determine the frequency of occurrence of HPV types 16 and 18 in liquid-based cytology (LBC) cervical samples in our set-up. This study comprised of 302 LBC cervical samples. HPV 16 and HPV 18 were detected by polymerase chain reaction (PCR), and the results were compared between normal (n = 155), inflammatory (n = 99), squamous (n = 37) and glandular abnormalities (n = 11). Of our patient cohort, 73.8 % was ≤40 years old. We found HPV 16 DNA in 91/302 (30.1 %) cases and HPV 18 DNA in 21/302 (6.95 %). HPV types 16 and 18 were detected in 25.8 and 4.5 % cytologically normal samples, respectively. HPV 16 was positive in 29.3 % of inflammatory samples. Squamous cervical abnormalities were more often HPV positive (HPV 16 in 48.6 %; HPV 18 in 29.7 %) than glandular abnormalities (36.4 and 18.2 %, respectively). We found high-risk HPV DNA in more than one third of the tested women. A good number of these HPV-positive cases were negative in cervical cytology.

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Authors and Affiliations

  1. Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

    Vikrant S. Bhar, Nalini Gupta, Raje Nijhawan, Radhika Srinivasan, Adrash Barward, Swati Sood & Arvind Rajwanshi

  2. Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

    Mini P. Singh

  3. Department of Obstetrics and Gynecology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

    Vanita Suri

Authors
  1. Vikrant S. Bhar
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  2. Nalini Gupta
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  3. Mini P. Singh
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  4. Raje Nijhawan
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  5. Radhika Srinivasan
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  6. Vanita Suri
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  7. Adrash Barward
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  8. Swati Sood
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  9. Arvind Rajwanshi
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Correspondence to Nalini Gupta.

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Bhar, V.S., Gupta, N., Singh, M.P. et al. Human papillomavirus (HPV) types 16 and 18 in liquid-based cervical cytology samples. Virchows Arch 466, 711–715 (2015). https://doi.org/10.1007/s00428-015-1750-7

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  • DOI: https://doi.org/10.1007/s00428-015-1750-7

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