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Plasmacytoid cells in salivary-gland pleomorphic adenomas: evidence of luminal cell differentiation

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Abstract

To determine the cellular origin of plasmacytoid cells in salivary gland adenomas, immunohistochemistry was performed on sections from 12 pleomorphic adenomas rich in these cells. In normal salivary glands included in these sections, the myoepithelial cells (MECs) expressed α-smooth muscle actin (αSMA) and smooth muscle myosin heavy chain (SMMHC), whereas the duct luminal cells expressed keratins 19, 18 and 8. Some of the salivary duct basal cells expressed these keratins, and the acinar cells expressed keratins 18 and 8. The expression profile was similar in rat salivary glands not only after but also during development. The immature MECs never expressed the keratins nor did the immature duct cells express αSMA. In seven cases, up to 60% of the plasmacytoid cells expressed keratin 19. In three of these cases, about 10% of the plasmacytoid cells expressed keratin 18. No plasmacytoid cells expressed αSMA, SMMHC or keratin 8. These results indicate that plasmacytoid cells originate from luminal cells and not from MECs. Furthermore, in addition to the luminal tumor cells, the non-luminal cells could express keratins 19, 18 and 8. Therefore, it is necessary to re-evaluate the prevailing notion that non-luminal cells are modified MECs. Keratin 14, basic calponin, vimentin and p63 were bi-specific for the MECs and the duct cells. Therefore, expression of these proteins by significant numbers of the non-luminal tumor cells and the plasmacytoid cells never denied the above notion.

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Acknowledgement

The authors thank Dr. Ross T. Fernley for his critical reading of the manuscript.

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Authors and Affiliations

  1. Department of Oral Pathology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, 565-0871, Osaka , Japan

    Yuzo Ogawa, Mitsunobu Kishino, Masaya Kimoto & Naokuni Ijuhin

  2. Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, Osaka, Japan

    Yukako Atsumi

  3. Clinical Laboratory, Osaka University Dental Hospital, Osaka, Japan

    Yasuo Fukuda & Takeshi Ishida

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  1. Yuzo Ogawa
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  2. Mitsunobu Kishino
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  3. Yukako Atsumi
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  4. Masaya Kimoto
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  5. Yasuo Fukuda
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Correspondence to Yuzo Ogawa.

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Ogawa, Y., Kishino, M., Atsumi, Y. et al. Plasmacytoid cells in salivary-gland pleomorphic adenomas: evidence of luminal cell differentiation. Virchows Arch 443, 625–634 (2003). https://doi.org/10.1007/s00428-003-0890-3

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  • DOI: https://doi.org/10.1007/s00428-003-0890-3

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