Abstract
Main conclusion
The rice OsFAH gene functions identically to that of Arabidopsis SSCD1 encoding FAH. Loss of OsFAH causes rice sterility.
Fumarylacetoacetate hydrolase (FAH) is the last enzyme in the tyrosine (Tyr) degradation pathway that is crucial for animals. By genetic analysis of the mutant of Short-day Sensitive Cell Death 1 gene encoding Arabidopsis FAH, we first found the pathway also plays a critical role in plants (Han et al., Plant Physiol 162:1956–1964, 2013). To further understand the role of the Tyr degradation pathway in plants, we investigated a biological function of the rice FAH. Firstly, the cDNA of rice FAH gene (OsFAH) was cloned and confirmed to be able to rescue the Arabidopsis Short-day Sensitive Cell Death 1 mutant defective in the FAH. Then, we identified the OsFAH T-DNA insertion mutant and generated the OsFAH RNA interference lines, and found that loss of OsFAH results in rice sterility. Furthermore, we analyzed expression of the OsFAH gene in roots, stems, leaves and young panicles at booting stage of rice and found that its transcript level was highest in young panicles and lowest in roots. In addition, the expression analysis of β-glucuronidase driven by OsFAH promoter in transgenic Arabidopsis showed that the OsFAH promoter was highly active in aerial tissues in vegetative stage, and sepals, filaments and stigma in reproductive stage. These results suggested that FAH plays an important role in rice fertility.
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Abbreviations
- FAH:
-
Fumarylacetoacetate hydrolase
- GSTZ:
-
The zeta class glutathione transferases
- GUS:
-
β-Glucuronidase
- RNAi:
-
RNA interference
- SSCD1 :
-
Short-day Sensitive Cell Death 1
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Acknowledgements
We thank Dr Gynheung An at Pohang University of Science and Technology/Kyung Hee University for providing the rice OsFAH T-DNA insertion line PFG_3D-01883, and Dr Guoliang Wang at Ohio State University for providing pANDA vector. The study was supported by a grant from the program for key basic research of the Ministry of Science and Technology of China (2014CB160308), a grant from Education Department of Hunan (18A100), and the Open Research Fund of Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley of Ministry of Agriculture in China (Hunan Rice Research Institute) (2018KLMA03).
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Hu, C., Huang, L., Chen, Y. et al. Fumarylacetoacetate hydrolase is required for fertility in rice. Planta 253, 122 (2021). https://doi.org/10.1007/s00425-021-03632-1
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DOI: https://doi.org/10.1007/s00425-021-03632-1