Abstract
Main conclusion
Comparative morphological, transcriptomic and phytohormone analyses reveal a defence network leading to PCD involved in cabbage hybrid lethality.
Abstract
Hybrid lethality (HL) plays an essential role in the stability of a population by blocking gene exchange between species, but the molecular mechanism remains largely undetermined. In this study, we performed phenotype, transcriptome and plant hormone analyses of HL in cabbage. Phenotype analysis confirmed that HL is characterised by a typical programmed cell death (PCD) process. A time-resolved RNA-Seq identified 2724 differentially expressed genes (DEGs), and functional annotations analyses revealed that HL was closely associated with the defence response. A defence regulation network was constructed based on the plant–pathogen interaction pathway and MAPK signalling pathway, which comprised DEGs related to Ca2+ and hydrogen peroxide (H2O2) leading to PCD. Moreover, important DEGs involved in hormone signal transduction pathways including salicylic acid (SA) and jasmonic acid (JA) were identified, which were further confirmed by endogenous and exogenous SA and JA measurements. Our results identified key genes and pathways in the regulating network of HL in cabbage, and might open the gate for revealing the molecular mechanism of HL in plants.
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Abbreviations
- DEG:
-
Differentially expressed gene
- HL:
-
Hybrid lethality
- HR:
-
Hypersensitive response
- JA:
-
Jasmonic acid
- PCD:
-
Programmed cell death
- SA:
-
Salicylic acid
- TF:
-
Transcription factor
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Acknowledgements
The work reported here was performed at the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing 100081, China.
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This work was financially supported by grants from the National Natural Science Foundation of China (31572139), the Key Projects of the National Key Research and Development Program of China (2016YFD0100307), the Central Public-interest Scientific Institution Basal Research Fund (Y2020PT01), and the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS).
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Xiao, Z., Liu, X., Fang, Z. et al. Transcriptome and plant hormone analyses provide new insight into the molecular regulatory networks underlying hybrid lethality in cabbage (Brassica oleracea). Planta 253, 96 (2021). https://doi.org/10.1007/s00425-021-03608-1
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DOI: https://doi.org/10.1007/s00425-021-03608-1