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The transcription factor MYB15 is essential for basal immunity (PTI) in Chinese wild grape

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Abstract

Main conclusion

MYB15 promoter of Vitis quinquangularis has potential as a target for disease resistance breeding, and its involvement in PTI is associated with a range of defense mechanisms.

China is a center of origin for Vitis and is home to diverse wild Vitis genotypes, some of which show superior pathogen resistance, although the underlying molecular basis for this has not yet been elucidated. In the current study, we identified a transcription factor, MYB15, from the Chinese wild grape, Vitis quinquangularis, whose promoter region (pVqMYB15) was shown to be induced by basal immunity (also called PAMP-triggered immunity, PTI) triggered by flg22, following heterologous expression in Nicotiana benthamiana and homologous expression in grapevine. By analyzing the promoter structure and activity, we identified a unique 283 bp sequence that plays a key role in the activation of basal immunity. In addition, we showed that activation of the MYB15 promoter correlates with differences in the expression of MYB15 and RESVERATROL SYNTHASE (RS) induced by the flg22 elicitor. We further tested whether the MYB15 induction triggered by flg22 was consistent with MYB15 and RS expression following inoculation with Plasmopara viticola in grape (V. quinquangularis and Vitis vinifera) leaves. Mapping upstream signals, we found that calcium influx, an RboH-dependent oxidative burst, an MAPK cascade, and jasmonate and salicylic acid co-contributed to flg22-triggered pVqMYB15 activation. Our data suggest that the MYB15 promoter has potential as a target for disease resistance breeding, and its involvement in PTI is associated with a range of defense mechanisms.

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Abbreviations

DPI:

Diphenylene-iodonium chloride

GUS:

β-Glucuronidase

MeJA:

Methyl jasmonate

PAMP:

Pathogen-associated molecular patterns

PD98059:

2-(2-Amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

PTI:

PAMP-triggered immunity

RS:

Resveratrol synthase

SA:

Salicylic acid

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (31600256), the Young Academic Talent Support Program of Northwest University (for Dong Duan), the Scientific Research Program Funded by Shaanxi Provincial Education Department (18JK0769), the Natural Science Basic Research Plan in Shaanxi Province of China (2017JQ3005), the Open Foundation of Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education) (ZSK2017003), the Graduate Student Independent Innovation Project of Northwest University (YZZ17168), and the Northwest University Training Program of Innovation and Entrepreneurship for Undergraduate (2018330 and 2018306).

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  1. Yangyang Luo, Ru Bai, and Jing Li have contributed equally to this work.

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  1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, 710069, Shaanxi, China

    Yangyang Luo, Ru Bai, Jing Li, Weidong Yang, Ruixiang Li, Qingyang Wang, Guifang Zhao & Dong Duan

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  1. Yangyang Luo
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Correspondence to Dong Duan.

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Luo, Y., Bai, R., Li, J. et al. The transcription factor MYB15 is essential for basal immunity (PTI) in Chinese wild grape. Planta 249, 1889–1902 (2019). https://doi.org/10.1007/s00425-019-03130-5

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