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Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana

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Abstract

Main conclusion

Expression of bHLH104 - GFP driven by the MYB72 promoter improves plants’ tolerance to Fe deficiency and increases seed Fe concentrations.

Iron (Fe) deficiency causes reduced crop yield and quality. In humans, Fe deficiency is directly associated with Fe-deficiency anemia. Therefore, breeding Fe-deficiency tolerant and Fe-enriched plants are an ideal approach to deal with these problems. Here, different strategies were explored to generate Fe-deficiency tolerant and Fe-enriched plants. Unexpectedly, the overexpression of Fe-deficiency responsive genes (IRT1, MYB72, and bHLH100) resulted in enhanced sensitivity to Fe deficiency, including leaf chlorosis and short roots under Fe-deficiency conditions. Next, three different types of Fe-deficiency responsive promoters (Pro IRT1 , Pro MYB72, and Pro bHLH100 ) were used to drive the expression of bHLH104-GFP fusion gene in Arabidopsis. Pro IRT1 :bHLH104-GFP plants showed the enhanced sensitivity to Fe deficiency on Fe-deficient media and the reduced fertility in alkaline soil. In contrast, Pro bHLH100 :bHLH104-GFP plants displayed a slight tolerance to Fe deficiency and Pro MYB72 :bHLH104-GFP plants had a significant advantage in growth in alkaline soil, including increased root length, chlorophyll, and biomass. Further analysis revealed that the expression of Fe-deficiency responsive genes was dramatically upregulated in both Pro MYB72 :bHLH104-GFP and Pro bHLH100 :bHLH104-GFP plants under Fe-deficiency conditions. When grown in alkaline soil, Pro MYB72 :bHLH104-GFP plants greatly improved the seed yield and Fe concentration. These results are fundamental for plant manipulation approaches to modify tolerance to Fe deficiency and Fe accumulation through alterations of bHLH104 gene expression.

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Abbreviations

IRT1:

IRON TRANSPORTER 1

FRO2:

FERRIC REDUCTION OXIDASE 2

FIT:

FER-LIKE REGULATOR OF IRON UPTAKE

NA:

Nicotianamine

NAS:

NICOTIANAMINE SYNTHASE

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Acknowledgments

This work was supported by the Candidates of the Young and Middle Aged Academic Leaders of Yunnan Province [2015HB095], the Youth Innovation Promotion Association of CAS, and the program for Innovative Research Team of Yunnan Province [2014HC017].

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Authors and Affiliations

  1. Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China

    Ce Wang, Xiani Yao, Diqiu Yu & Gang Liang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Ce Wang & Xiani Yao

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  1. Ce Wang
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  2. Xiani Yao
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  3. Diqiu Yu
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  4. Gang Liang
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Correspondence to Diqiu Yu or Gang Liang.

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Wang, C., Yao, X., Yu, D. et al. Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana . Planta 246, 421–431 (2017). https://doi.org/10.1007/s00425-017-2703-y

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