Skip to main content
SpringerLink
Log in

Genetic analysis of a novel broad-spectrum powdery mildew resistance gene from the wheat-Agropyron cristatum introgression line Pubing 74

  • Original Article
  • Published:
Planta Aims and scope Submit manuscript

Abstract

Main conclusion

A novel broad-spectrum powdery mildew resistance gene PmPB74 was identified in wheat- Agropyron cristatum introgression line Pubing 74.

Development of wheat cultivars with broad-spectrum, durable resistance to powdery mildew has been restricted by lack of superior genetic resources. In this study, a wheat-A. cristatum introgression line Pubing 74, originally selected from a wide cross between the common wheat cultivar Fukuhokomugi (Fukuho) and Agropyron cristatum (L.) Gaertn (2n = 4x = 28; genome PPPP), displayed resistance to powdery mildew at both the seedling and adult stages. The putative alien chromosomal fragment in Pubing 74 was below the detection limit of genomic in situ hybridization (GISH), but evidence for other non-GISH-detectable introgressions was provided by the presence of three STS markers specific to A. cristatum. Genetic analysis indicated that Pubing 74 carried a single dominant gene for powdery mildew resistance, temporarily designated PmPB74. Molecular mapping showed that PmPB74 was located on wheat chromosome arm 5DS, and flanked by markers Xcfd81 and HRM02 at genetic distances of 2.5 and 1.7 cM, respectively. Compared with other lines with powdery mildew resistance gene(s) on wheat chromosome arm 5DS, Pubing 74 was resistant to all 28 Blumeria graminis f. sp tritici (Bgt) isolates from different wheat-producing regions of northern China. Allelism tests indicated that PmPB74 was not allelic to PmPB3558 or Pm2. Our work showed that PmPB74 is a novel gene with broad resistance to powdery mildew, and hence will be helpful in broadening the genetic basis of powdery mildew resistance in wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

Fukuho:

cv. Fukuhokomugi

GISH:

Genomic in situ hybridization

HRM:

High resolution DNA melting

SNP:

Single nucleotide polymorphism

SSR:

Simple sequence repeat

References

  • Akhunov E, Nicolet C, Dvorak J (2009) Single nucleotide polymorphism genotyping in polyploid wheat with the Illumina GoldenGate assay. Theor Appl Genet 119:507–517

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Allen GC, Flores-Vergara MA, Krasynanski S, Kumar S, Thompson WF (2006) A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nat Protoc 1:2320–2325

    Article  CAS  PubMed  Google Scholar 

  • Allen AM, Barker GL, Wilkinson P, Burridge A, Winfield M, Coghill J, Uauy C, Griffiths S, Jack P, Berry S, Werner P, Melichar JP, McDougall J, Gwilliam R, Robinson P, Edwards K (2013) Discovery and development of exome-based, co-dominant single nucleotide polymorphism markers in hexaploid wheat (Triticum aestivum L.). Plant Biotechnol J 11:279–295

    Article  CAS  PubMed  Google Scholar 

  • Avni R, Nave M, Eilam T, Sela H, Alekperov C, Peleg Z, Dvorak J, Korol A, Distelfeld A (2014) Ultra-dense genetic map of durum wheat × wild emmer wheat developed using the 90K iSelect SNP genotyping assay. Mol Breed 34:1–14

    Article  Google Scholar 

  • Berard A, Le Paslier MC, Dardevet M, Exbrayat-Vinson F, Bonnin I, Cenci A, Haudry A, Brunel D, Ravel C (2009) High-throughput single nucleotide polymorphism genotyping in wheat (Triticum spp.). Plant Biotechnol J 7:364–374

    Article  CAS  PubMed  Google Scholar 

  • Bowen KL, Everts KL, Leath S (1991) Reduction in yield of winter wheat in North Carolina due to powdery mildew and leaf rust. Phytopathology 81:503–511

    Article  Google Scholar 

  • Briggle LW (1966) Three loci in wheat involving resistance to Erysiphe graminis f. sp. tritici. Crop Sci 6:461–465

    Article  Google Scholar 

  • Caceres ME, Pupilli F, Ceccarelli M, Vaccino P, Sarri V, Depace C, Cionini PG (2012) Cryptic introgression of Dasypyrum villosum parental DNA in wheat lines derived from intergeneric hybridization. Cytogenet Genome Res 136:75–81

    Article  CAS  PubMed  Google Scholar 

  • Cavanagh CR, Chao SM, Wang SC, Huang BE, Stephen S et al (2013) Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proc Natl Acad Sci USA 110:8057–8062

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chao S, Dubcovsky J, Dvorak J, Luo MC, Baenziger SP et al (2010) Population- and genome-specific patterns of linkage disequilibrium and SNP variation in spring and winter wheat (Triticum aestivum L.). BMC Genom 11:727

    Article  CAS  Google Scholar 

  • Chen PD, Qi LL, Zhou B, Zhang SZ, Liu DJ (1995) Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet 91:1125–1128

    CAS  PubMed  Google Scholar 

  • Chen G, Zheng Q, Bao YG, Liu SB, Wang HG, Li XF (2012) Molecular cytogenetic identification of a novel dwarf wheat line with introgressed Thinopyrum ponticum chromatin. J Biol Sci 37:149–155

    Google Scholar 

  • Chen PD, You CF, Hu Y, Chen SW, Zhou B, Cao AZ, Wang X (2013) Radiation-induced translocations with reduced Haynaldia villosa chromatin at the Pm21 locus for powdery mildew resistance in wheat. Mol Breed 31:477–484

    Article  CAS  Google Scholar 

  • Dewey DR (1984) The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson JP (ed) Gene manipulation in plant inprovement. Plenum Press, New York, pp 209–279

    Chapter  Google Scholar 

  • Dong YC, Zhou RH, Xu SJ, Li LH, Cauderon Y, Wang RRC (1992) Desirable characteristics in perennial Triticeae collected in China for wheat improvement. Hereditas 116:175–178

    Article  Google Scholar 

  • Everts KL, Leath S (1992) Effect of early season powdery mildew on development, survival, and yield contribution of tillers of winter wheat. Phytopathology 82:1273–1278

    Article  Google Scholar 

  • Friebe B, Heun M, Tuleen N, Zeller FJ, Gill BS (1994) Cytogenetically monitored transfer of powdery mildew resistance from rye into wheat. Crop Sci 34:621–625

    Article  Google Scholar 

  • Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87

    Article  Google Scholar 

  • Gao HD, Zhu FF, Jiang YJ, Wu JZ, Yan W, Zhang QF, Jacobi A, Cai SB (2012) Genetic analysis and molecular mapping of a new powdery mildew resistant gene Pm46 in common wheat. Theor Appl Genet 125:967–973

    Article  CAS  PubMed  Google Scholar 

  • Gill BS, Friebe BR, White FF (2011) Alien introgressions represent a rich source of genes for crop improvement. Proc Natl Acad Sci USA 108:7657–7658

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Han HM, Bai L, Su JJ, Zhang JP, Song LQ, Gao AN, Yang XM, Li XQ, Liu WH, Li LH (2014) Genetic rearrangements of six wheat-Agropyron cristatum 6P addition lines revealed by molecular markers. PLoS One 9:e91066

    Article  PubMed  PubMed Central  Google Scholar 

  • He RL, Chang ZJ, Yang ZJ, Yuan ZY, Zhan HX, Zhang XJ, Liu JX (2009) Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat. Theor Appl Genet 118:1173–1180

    Article  CAS  PubMed  Google Scholar 

  • Hsam SLK, Zeller FJ (2002) Breeding for powdery mildew resistance in common wheat (T. aestivum L.). In: Belanger RR, Bushnell WR, Dik AJ, Carver TLW (eds) The powdery mildews, a comprehensive treatise. APS Press, St Paul, pp 219–238

    Google Scholar 

  • Huang XQ, Roder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica 137:203–223

    Article  CAS  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ (1997) Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L. em Thell).9. Cultivars, land races and breeding lines grown in China. Plant Breed 116:233–238

    Article  Google Scholar 

  • Huang J, Zhao ZH, Song FJ, Wang XM, Xu HX, Huang Y, An DG, Li HJ (2012) Molecular detection of a gene effective against powdery mildew in the wheat cultivar Liangxing 66. Mol Breed 30:1737–1745

    Article  CAS  Google Scholar 

  • Huang Q, Li X, Chen WQ, Xiang ZP, Zhong SF, Chang ZJ, Zhang M, Zhang HY, Tan FQ, Ren ZL, Luo PG (2014) Genetic mapping of a putative Thinopyrum intermedium-derived stripe rust resistance gene on wheat chromosome 1B. Theor Appl Genet 127:843–853

    Article  CAS  PubMed  Google Scholar 

  • Jauhar PP, Peterson TS (2006) Cytological analyses of hybrids and derivatives of hybrids between durum wheat and Thinopyrum bessarabicum, using multi-colour fluorescent GISH. Plant Breed 125:19–26

    Article  Google Scholar 

  • Jia JZ, Zhao SC, Kong XY, Li YR, Zhao GY et al (2013) Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation. Nature 496:91–95

    Article  CAS  PubMed  Google Scholar 

  • Kuraparthy V, Chhuneja P, Dhaliwal HS, Kaur S, Bowden RL, Gill BS (2007a) Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. Theor Appl Genet 114:1379–1389

    Article  CAS  PubMed  Google Scholar 

  • Kuraparthy V, Sood S, Chhuneja P, Dhaliwal HS, Kaur S, Bowden RL, Gill BS (2007b) A cryptic wheat-Aegilops triuncialis translocation with leaf rust resistance gene Lr58. Crop Sci 47:1995–2003

    Article  CAS  Google Scholar 

  • Lai KT, Duran C, Berkman PJ, Lorenc MT, Stiller J, Manoli S, Hayden MJ, Forrest KL, Fleury D, Baumann U, Zander M, Mason AS, Batley J, Edwards D (2012) Single nucleotide polymorphism discovery from wheat next-generation sequence data. Plant Biotechnol J 10:743–749

    Article  CAS  PubMed  Google Scholar 

  • Limpert E, Andrivon D, Felsenstein FG (1988) Influence of different benzimidazole concentrations in agar medium on senescence of wheat leaf segments and on growth and sporulation of the wheat powdery mildew pathogen. J Plant Dis Protect 95:301–306

    CAS  Google Scholar 

  • Lincoln SE, Daly MJ, Lander ES (1993) Constructing linkage maps with MAPMAKER/Exp version 3. 0: a tutorial reference manual, 3rd edn. Whitehead Institute for Medical Res, Cambridge

    Google Scholar 

  • Liu RH, Meng JL (2003) MapDraw: a microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas 25:317–321

    PubMed  Google Scholar 

  • Liu ZY, Sun QX, Ni ZF, Nevo E, Yang TM (2002) Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica 123:21–29

    Article  CAS  Google Scholar 

  • Liu J, Chang ZJ, Zhang XJ, Yang ZJ, Li X, Jia JQ, Zhan HX, Guo HJ, Wang JM (2013) Putative Thinopyrum intermedium-derived stripe rust resistance gene Yr50 maps on wheat chromosome arm 4BL. Theor Appl Genet 126:265–274

    Article  CAS  PubMed  Google Scholar 

  • Liu ZH, Xu M, Xiang ZP, Li X, Chen WQ, Luo PG (2014) Registration of the novel wheat lines L658, L693, L696, and L699, which are resistant to Fusarium head blight, stripe rust, and powdery mildew. J Plant Regist 9:121–124

    Article  Google Scholar 

  • Lu YQ, Wu XY, Yao MM, Zhang JP, Liu WH, Yang XM, Li XQ, Du J, Gao AN, Li LH (2015) Genetic mapping of a putative Agropyron cristatum-derived powdery mildew resistance gene by a combination of bulked segregant analysis and single nucleotide polymorphism array. Mol Breed 35:1–13

    Article  Google Scholar 

  • Luan Y, Wang XG, Liu WH, Li CY, Zhang JP, Gao AN, Wang YD, Yang XM, Li LH (2010) Production and identification of wheat-Agropyron cristatum 6P translocation lines. Planta 232:501–510

    Article  CAS  PubMed  Google Scholar 

  • Ma HQ, Kong ZX, Fu BS, Li N, Zhang LX, Jia HY, Ma ZQ (2011) Identification and mapping of a new powdery mildew resistance gene on chromosome 6D of common wheat. Theor Appl Genet 123:1099–1106

    Article  CAS  PubMed  Google Scholar 

  • Ma PT, Xu HX, Luo QL, Qie YM, Zhou YL, Xu YF, Han HM, Li LH, An DG (2014) Inheritance and genetic mapping of a gene for seedling resistance to powdery mildew in wheat line X3986-2. Euphytica 200:149–157

    Article  CAS  Google Scholar 

  • Ma PT, Xu HX, Xu YF, Li LL, Qie YM, Luo QL, Zhang XT, Li XQ, Zhou YL, An DG (2015) Molecular mapping of a new powdery mildew resistance gene Pm2b in Chinese breeding line KM2939. Theor Appl Genet 128:613–622

    Article  CAS  PubMed  Google Scholar 

  • Matsuda R, Iehisa JC, Takumi S (2012) Application of real-time PCR-based SNP detection for mapping of Net2, a causal D-genome gene for hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii. Genes Genet Syst 87:137–143

    Article  CAS  PubMed  Google Scholar 

  • McDonald BA, Linde C (2002) The population genetics of plant pathogens and breeding strategies for durable resistance. Euphytica 124:163–180

    Article  CAS  Google Scholar 

  • McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2014) Catalogue of gene symbols for wheat: 2013–2014 supplement. Komugi-wheat genetic resources database. http://www.shigen.nig.ac.jp/wheat/komugi/

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Miranda LM, Murphy JP, Marshall D, Leath S (2006) Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theor Appl Genet 113:1497–1504

    Article  CAS  PubMed  Google Scholar 

  • Miranda LM, Murphy JP, Marshall D, Cowger C, Leath S (2007) Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theor Appl Genet 114:1451–1456

    Article  CAS  PubMed  Google Scholar 

  • Mohler V, Hsam SLK, Zeller FJ, Wenzel G (2001) An STS marker distinguishing the rye-derived powdery mildew resistance alleles at the Pm8/Pm17 locus of common wheat. Plant Breed 120:448–450

    Article  CAS  Google Scholar 

  • Mohler V, Bauer C, Schweizer G, Kempf H, Hartl L (2013) Pm50: a new powdery mildew resistance gene in common wheat derived from cultivated emmer. J Appl Genet 54:259–263

    Article  CAS  PubMed  Google Scholar 

  • Paillard S, Goldringer I, Enjalbert J, Doussinault G, de Vallavieille-Pope C, Brabant P (2000) Evolution of resistance against powdery mildew in winter wheat populations conducted under dynamic management. I–Is specific seedling resistance selected? Theor Appl Genet 101:449–456

    Article  CAS  Google Scholar 

  • Paillard S, Schnurbusch T, Winzeler M, Messmer M, Sourdille P, Abderhalden O, Keller B, Schachermayr G (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.). Theor Appl Genet 107:1235–1242

    Article  CAS  PubMed  Google Scholar 

  • Parks R, Carbone I, Murphy JP, Marshall D, Cowger C (2008) Virulence structure of the Eastern US wheat powdery mildew population. Plant Dis 92:1074–1082

    Article  Google Scholar 

  • Petersen S, Lyerly JH, Worthington ML, Parks WR, Cowger C, Marshall DS, Brown-Guedira G, Murphy PJ (2015) Mapping of powdery mildew resistance gene Pm53 introgressed from Aegilops speltoides into soft red winter wheat. Theor Appl Genet 128:303–312

    Article  CAS  PubMed  Google Scholar 

  • Qiu YC, Sun XL, Zhou RH, Kong XY, Zhang SS, Jia JZ (2006) Identification of microsatellite markers linked to powdery mildew resistance gene Pm2 in wheat. Cereal Res Commun 34:1267–1273

    Article  CAS  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  PubMed Central  Google Scholar 

  • Schmolke M, Mohler V, Hartl L, Zeller FJ, Hsam SLK (2012) A new powdery mildew resistance allele at the Pm4 wheat locus transferred from einkorn (Triticum monococcum). Mol Breed 29:449–456

    Article  CAS  Google Scholar 

  • Schneider A, Molnar I, Molnar-Lang M (2008) Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. Euphytica 163:1–19

    Article  CAS  Google Scholar 

  • Shen XK, Ma LX, Zhong SF, Liu N, Zhang M, Chen WQ, Zhou YL, Li HJ, Chang ZJ, Li X, Bai GH, Zhang HY, Tan FQ, Ren ZL, Luo PG (2015) Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS. Theor Appl Genet 128:517–528

    Article  CAS  PubMed  Google Scholar 

  • Shi AN, Leath S, Murphy JP (1998) A major gene for powdery mildew resistance transferred to common wheat from wild einkorn wheat. Phytopathology 88:144–147

    Article  CAS  PubMed  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi LL, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of geneticphysical map relationships in wheat (Triticum aestivum L.). Funct Integr Genom 4:12–25

    Article  CAS  Google Scholar 

  • Sun YL, Zou JW, Sun HG, Song W, Wang XM, Li HJ (2015) PmLX66 and PmW14: new alleles of Pm2 for resistance to powdery mildew in the Chinese winter wheat cultivars Liangxing 66 and Wennong 14. Plant Dis 99:1118–1124

    Article  CAS  Google Scholar 

  • Tan YY, Fu HW, Zhao HR, Lu S, Fu JJ, Li YF, Cui HR, Shu QY (2013) Functional molecular markers and high-resolution melting curve analysis of low phytic acid mutations for marker-assisted selection in rice. Mol Breed 31:517–528

    Article  CAS  Google Scholar 

  • Terracciano I, Maccaferri M, Bassi F, Mantovani P, Sanguineti MC, Salvi S, Simkova H, Dolezel J, Massi A, Ammar K, Kolmer J, Tuberosa R (2013) Development of COS-SNP and HRM markers for high-throughput and reliable haplotype-based detection of Lr14a in durum wheat (Triticum durum Desf.). Theor Appl Genet 126:1077–1101

    Article  CAS  PubMed  Google Scholar 

  • Wang SC, Wong D, Forrest K, Allen A, Chao SM et al (2014) Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wu J, Yang XM, Wang H, Li HJ, Li LH, Li XQ, Liu WH (2006) The introgression of chromosome 6P specifying for increased numbers of florets and kernels from Agropyron cristatum into wheat. Theor Appl Genet 114:13–20

    Article  CAS  PubMed  Google Scholar 

  • Xie WL, Ben-David R, Zeng B, Dinoor A, Xie CJ, Sun QX, Röder MS, Fahoum A, Fahima T (2012) Suppressed recombination rate in 6VS/6AL translocation region carrying the Pm21 locus introgressed from Haynaldia villosa into hexaploid wheat. Mol Breeding 29:399–412

    Article  CAS  Google Scholar 

  • Yao GQ, Zhang JL, Yang LL, Xu HX, Jiang YM, Xiong L, Zhang CQ, Zhang ZZ, Ma ZQ, Sorrells ME (2007) Genetic mapping of two powdery mildew resistance genes in einkorn (Triticum monococcum L.) accessions. Theor Appl Genet 114:351–358

    Article  CAS  PubMed  Google Scholar 

  • Ye XL, Lu YQ, Liu WH, Chen GY, Han HM, Zhang JP, Yang XM, Li XQ, Gao AN, Li LH (2015) The effects of chromosome 6P on fertile tiller number of wheat as revealed in wheat-Agropyron cristatum chromosome 5A/6P translocation lines. Theor Appl Genet 128:797–811

    Article  PubMed  Google Scholar 

  • Yu JK, Dake TM, Singh S, Benscher D, Li W, Gill B, Sorrells ME (2004) Development and mapping of EST-derived simple sequence repeat markers for hexaploid wheat. Genome 47: 805–818

    Article  CAS  PubMed  Google Scholar 

  • Zhang RQ, Wang X, Chen PD (2012) Molecular and cytogenetic characterization of a small alien-segment translocation line carrying the softness genes of Haynaldia villosa. Genome 55:639–646

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Zhang JP, Liu WH, Han HM, Lu YQ, Yang XM, Li XQ, Li LH (2015a) Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length. Theor Appl Genet 128:1827–1837

    Article  PubMed  Google Scholar 

  • Zhang JP, Liu WH, Han HM, Song LQ, Bai L, Gao ZH, Zhang Y, Yang XM, Li XQ, Gao AN, Li LH (2015b) De novo transcriptome sequencing of Agropyron cristatum to identify available gene resources for the enhancement of wheat. Genomics 106:129–136

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was funded by the National Natural Science Foundation of China (No. 31271714), the National Key Technology Support Program of China (No. 2013BAD01B02), and the CAAS Innovation Team Project.

Author information

Author notes

    Authors and Affiliations

    1. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

      Yuqing Lu, Miaomiao Yao, Jinpeng Zhang, Liqiang Song, Weihua Liu, Xinming Yang, Xiuquan Li & Lihui Li

    Authors
    1. Yuqing Lu
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Miaomiao Yao
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Jinpeng Zhang
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Liqiang Song
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Weihua Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Xinming Yang
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Xiuquan Li
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Lihui Li
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Lihui Li.

    Ethics declarations

    Conflict of interest

    The authors declare that they have no conflicts of interest.

    Additional information

    Y. Lu and M. Yao are contributed equally to this work.

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Lu, Y., Yao, M., Zhang, J. et al. Genetic analysis of a novel broad-spectrum powdery mildew resistance gene from the wheat-Agropyron cristatum introgression line Pubing 74. Planta 244, 713–723 (2016). https://doi.org/10.1007/s00425-016-2538-y

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s00425-016-2538-y

    Keywords

    Search

    Navigation