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Dynamics of flavonol accumulation in leaf tissues under different UV-B regimes in Centella asiatica (Apiaceae)

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Abstract

Main conclusion

A cumulative effect of UV-B doses on epidermal flavonol accumulation was observed during the first week of a time course study in Centella asiatica (Apiaceae). However, once flavonol levels had peaked, additional accumulation was possible only if higher daily UV-B irradiances were applied.

We aimed to understand the dynamics of flavonol accumulation in leaf tissues using non-destructive spectroscopy and HPLC–mass spectrometry. When leaves that had grown without UV-B were given brief daily exposures to low-irradiance UV-B, they accumulated flavonols, predominantly kaempferol-3-O-β-d-glucuronopyranoside and quercetin-3-O-β-d-glucuronopyranoside, in their exposed epidermis, reaching a plateau after 7 days. More prolonged UV-B exposures or higher doses eventually augmented flavonol concentrations even in non-exposed tissues. If UV-B irradiance was subsequently reduced, leaves appeared to lose their ability to accumulate further flavonols in their epidermis even if the duration of daily exposure was increased. A higher irradiance level was then necessary to further increase flavonol accumulation. When subsequently acclimated to higher UV-B irradiances, mature leaves accumulated less flavonols than did developing ones. Our study suggests that levels of epidermal flavonols in leaves are governed primarily by UV-B irradiance rather than by duration of exposure.

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Abbreviations

Amu:

Atomic mass unit

AU:

Absorbance units

ChlF:

Chlorophyll a fluorescence

ChlF(ex375), (ex655),:

Chlorophyll a fluorescence induced by 375 nm or by 655 nm excitation wavelength

COP1 :

CONSTITUTIVELY PHOTOMORPHOGENIC1 gene

DPBA:

Diphenyl boric acid-2-aminoethyl ester

DA375 :

Dualex absorbance at 375 nm

LC–MS:

Liquid chromatography–mass spectrometry

PAR:

Photosynthetically active radiation

ROS:

Reactive oxygen species

SIR:

Single-ion record

RUP1:

REPRESSOR OF UV-B PHOTOMORPHOGENESIS1 protein

RUP2:

REPRESSOR OF UV-B PHOTOMORPHOGENESIS2 protein

UVR8 :

UV RESISTANCE LOCUS8 gene

UV-A:

Ultraviolet-A radiation (315–400 nm)

UV-B:

Ultraviolet-B radiation (280–315 nm)

SAA:

Systemic acquired acclimation

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Acknowledgments

We thank the Sotramex Company (Madagascar), which kindly provided Centella asiatica from Madagascar. This work was funded by the bilateral contract BISCAP 4 & 5 no 130913 between Bayer HealthCare and University of Montpellier during the period 2008–2013.

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Authors and Affiliations

  1. INRA, UMR AGAP, Centre de Recherche de Montpellier, 2 Place Pierre Viala-Bât. 21, 34060, Montpellier, France

    Luc P. R. Bidel

  2. Systematic Botany and Mycology, Department of Biology, University of Munich (LMU), 80638, Munich, Germany

    Guillaume Chomicki

  3. Laboratoire de Botanique, Phytochimie et Mycologie, UMR 5175 CEFE-CNRS, Faculté de Pharmacie, BP 14491, 34093, Montpellier Cedex 5, France

    Fabien Bonini, Laurence Mondolot & Philippe La Fisca

  4. Centre d’Immunologie de Marseille-Luminy, Parc Scientifique & Technologique de Luminy, 13288, Marseille Cedex 09, France

    Julien Soulé

  5. UMR DIADE (UM2-IRD), SMART Team cc024, University of Montpellier, Place Eugène Bataillon, 34095, Montpellier Cedex 05, France

    Marc Coumans, Yves Baissac & Christian Jay-Allemand

  6. Bayer HealthCare SAS Division Serdex, 1, Avenue Normandie-Niémen, 64140, Lons, France

    Virginie Petit & Alain Loiseau

  7. Equipe Biospectroscopie Végétale (CNRS), Laboratoire Ecologie Systématique Evolution, Département Ecophysiologie Végétale, UMR 8079 (CNRS, UPS, AgroParisTech), Université Paris-Sud 11, Orsay, France

    Zoran G. Cerovic

  8. School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington, New Zealand

    Kevin S. Gould

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  1. Luc P. R. Bidel
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  2. Guillaume Chomicki
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  3. Fabien Bonini
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Correspondence to Luc P. R. Bidel.

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Special topic: Polyphenols: biosynthesis and function in plants and ecosystems. Guest editor: Stefan Martens.

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Bidel, L.P.R., Chomicki, G., Bonini, F. et al. Dynamics of flavonol accumulation in leaf tissues under different UV-B regimes in Centella asiatica (Apiaceae). Planta 242, 545–559 (2015). https://doi.org/10.1007/s00425-015-2291-7

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