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Identification of differentially expressed transcripts associated with bast fibre development in Corchorus capsularis by suppression subtractive hybridization

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Abstract

Main conclusion

The present study documented the predominant role of WRKY transcription factor in controlling genes of different pathways related to fibre formation in jute and could be a candidate gene for the improvement of jute fiber.

Abstract

Understanding of molecular mechanism associated with bast fibre development is of immense significance to achieve desired improvement in jute (Corchorus sp.). Therefore, suppression subtractive hybridization was successfully applied to identify genes involved in fibre developmental process in jute. The subtracted library of normal Corchorus capsularis as tester with respect to its fibre-deficient mutant as driver resulted in 2,685 expressed sequence tags which were assumed to represent the differentially expressed genes between two genotypes. The identified expressed sequence tags were assembled and clustered into 225 contigs and 231 singletons. Among these 456 unigenes, 377 were classified into 15 different functional categories while others were of unknown functional category. Reverse Northern analysis of the unigenes showed distinct variation in hybridization intensity of 11 transcripts between two genotypes tested. The findings were also documented by Northern and real-time PCR analysis. Varied expression level of these transcripts suggested their crucial involvement in fibre development in this species. Among these transcripts, WRKY transcription factor was documented to be a most important transcript which was in agreement with its known role in other plant species in possible regulation related to cell wall biosynthesis, expansion and lignification. This report constitutes first systematic analysis of genes involved in fibre development process in jute. It may be suggested that the information generated in this study would be useful for genetic improvement of fibre traits in this plant species.

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Abbreviations

AGP:

Arabinogalactan protein

CAD:

Cinnamyl alcohol dehydrogenase

CCR:

Cinnamoyl-CoA reductase

C4H:

Cinnamate-4-hydroxylase

CHS:

Chalcone synthase

4CL:

4-Coumarate CoA:ligase

COMT:

Caffeic acid 3-O-methyltransferase

Das:

Days after sowing

EST:

Expressed sequence tags

FF:

Fibre forming

F5H:

Ferulic acid/coniferaldehyde/coniferyl alcohol 5-hydoxylase

GRP:

Glycine rich protein

HCT:

Hydroxycinnamoyl CoA:shikimate hydroxycinnamoyl transferase

HD-Zip:

Homeodomain leucine zipper protein

PAL:

Phenylalanine ammonia lyase

PEL:

Pectate lyase

PME:

Pectin methylesterase

SSH:

Suppression subtractive hybridization

XET:

Xyloglucan endotransglycosylase

ZFP:

Zinc finger protein

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Acknowledgments

Financial assistance in the form the grant support to this laboratory from the Department of Biotechnology, Government of India is acknowledged.

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  1. Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India

    Pradipta Samanta, Sanjoy Sadhukhan & Asitava Basu

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  1. Pradipta Samanta
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Correspondence to Asitava Basu.

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Samanta, P., Sadhukhan, S. & Basu, A. Identification of differentially expressed transcripts associated with bast fibre development in Corchorus capsularis by suppression subtractive hybridization. Planta 241, 371–385 (2015). https://doi.org/10.1007/s00425-014-2187-y

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