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Constitutive S-adenosylmethionine decarboxylase gene expression increases drought tolerance through inhibition of reactive oxygen species accumulation in Arabidopsis

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Abstract

Using subtractive hybridization analysis, the S-adenosylmethionine decarboxylase (SAMDC) gene from Capsicum annuum was isolated and renamed CaSAMDC. We generated independent transgenic Arabidopsis (Arabidopsis thaliana) lines constitutively expressing a 35S::CaSAMDC construct. Drought tolerance was significantly enhanced in Arabidopsis T4 transgenic homozygous lines as compared to wild-type (WT) plants. The levels of main polyamines (PAs) were more significantly increased in CaSAMDC-overexpressing transgenic plants after 6 h of drought stress as compared to stressed WT plants. Basal transcription of polyamine oxidase (PAO) showed at a much higher level in unstressed-transgenic plants as compared to unstressed WT plants. However, the difference in PAO transcription level between WT and transgenic plants was reduced after drought stress. Cellular accumulation of reactive oxygen species (ROS) was significantly reduced following drought stress in transgenic Arabidopsis plants as compared to WT plants. These results were in agreement with additional observations that stress-induced ROS generation, as determined by qRT-PCR analysis of NADPH oxidase (RbohD and RbohF), was significantly suppressed while transcription of ROS-detoxifying enzymes was notably elevated in transgenic lines in response to drought stress. Further, ROS-induced transcription of the metacaspase II gene was remarkably inhibited in transgenic plants. Collectively, these results suggest that drought stress tolerance due to reduction of ROS production and enhancement of ROS detoxification can be attributed to elevation of PAs.

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Abbreviations

PA:

Polyamine

ROS:

Reactive oxygen species

SAMDC:

S-Adenosylmethionine decarboxylase

ADC:

Arginine decarboxylase

SAM:

S-Adenosylmethionine

DAO:

Diamine oxidase

PAO:

Polyamine oxidase

DCFH-DA:

2′,7′-Dichlorodihydrofluorescein diacetate

APX:

Ascorbate peroxidase

MC:

Metacaspase

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Acknowledgments

This work was supported by grants from the National Research Foundation of Korea (Grant No. NRF-2011-0014857) to K.Y.P., the National Research Foundation (Project No. 2010-0000782 funded by the Ministry of Education, Science, and Technology, Republic of Korea), and the National Center for GM Crops (Project No. PJ008152 of the Next Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea) to W.T.K.

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Authors and Affiliations

  1. Department of Biology, Sunchon National University, Sunchon, Chonnam, 540-742, Korea

    Soo Jin Wi & Ky Young Park

  2. Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Korea

    Soo Jin Kim & Woo Taek Kim

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  1. Soo Jin Wi
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  2. Soo Jin Kim
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  3. Woo Taek Kim
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Correspondence to Woo Taek Kim or Ky Young Park.

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S. J. Wi and S. J. Kim contributed equally to this work.

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Wi, S.J., Kim, S.J., Kim, W.T. et al. Constitutive S-adenosylmethionine decarboxylase gene expression increases drought tolerance through inhibition of reactive oxygen species accumulation in Arabidopsis. Planta 239, 979–988 (2014). https://doi.org/10.1007/s00425-014-2027-0

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