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Transcriptional response of Medicago truncatula sulphate transporters to arbuscular mycorrhizal symbiosis with and without sulphur stress

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Abstract

Sulphur is an essential macronutrient for plant growth, development and response to various abiotic and biotic stresses due to its key role in the biosynthesis of many S-containing compounds. Sulphate represents a very small portion of soil S pull and it is the only form that plant roots can uptake and mobilize through H+-dependent co-transport processes implying sulphate transporters. Unlike the other organically bound forms of S, sulphate is normally leached from soils due to its solubility in water, thus reducing its availability to plants. Although our knowledge of plant sulphate transporters has been growing significantly in the past decades, little is still known about the effect of the arbuscular mycorrhiza interaction on sulphur uptake. Carbon, nitrogen and sulphur measurements in plant parts and expression analysis of genes encoding putative Medicago sulphate transporters (MtSULTRs) were performed to better understand the beneficial effects of mycorrhizal interaction on Medicago truncatula plants colonized by Glomus intraradices at different sulphate concentrations. Mycorrhization significantly promoted plant growth and sulphur content, suggesting increased sulphate absorption. In silico analyses allowed identifying eight putative MtSULTRs phylogenetically distributed over the four sulphate transporter groups. Some putative MtSULTRs were transcribed differentially in roots and leaves and affected by sulphate concentration, while others were more constitutively transcribed. Mycorrhizal-inducible and -repressed MtSULTRs transcripts were identified allowing to shed light on the role of mycorrhizal interaction in sulphate uptake.

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Abbreviations

AM:

Arbuscular mycorrhiza

AtSULTR:

Arabidopsis thaliana sulphate transporter

CNS:

Carbon, nitrogen, sulphur

CT:

Cycle threshold

DW:

Dry weight

FW:

Fresh weight

Mt:

Medicago truncatula

MtBcp1a :

Medicago truncatula blue copper protein 1a

MtPT4 :

Medicago truncatula phosphate transporter 4

MtSULTR:

Medicago truncatula sulphate transporter

Myc:

Mycorrhized

Myc:Myc:

Mycorrhized compartments of Myc plants

NM:

Non-mycorrhized

NM:Myc:

Non-mycorrhized compartments of Myc plants

NM:NM:

Non-mycorrhized compartments of NM plants

OsSULTR:

Orzya sativa sulphate transporter

qPCR:

Quantitative real time polymerase chain reaction

SULTR:

Sulphate transporter

Wpi:

Weeks post inoculation

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Acknowledgments

The present work was supported by funding from the Regional Council of Burgundy (2009–9201AAO040S00680) and by the National Agency for Research [ANR-10-BLAN-1604-01(TRANSMUT)]. The authors would like to thank Prof. J.-C.Davidian (BPMP Montpellier, France) for the discussions on S-metabolism and SULTRs’ importance in other model plants, C. Gutjahr (LMU Munich, Germany) for the help regarding plant physiology and plant’s responses to AM interaction, and the following members of the UMR Agroecology Dijon France: R. Thompson and C. Le Signor for helpful discussions throughout the project, D. van Tuinen for the bio-informatic help and the comments about plant’s SULTRs phylogeny, C. Schneider for creating scripts allowing analyses of leaf surface, H. Stockinger for the help with image format, A-L Santoni for the elemental analyses, A. Colombet and V. Monfort for providing the inoculum.

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  1. Pôle Interactions Plantes-Microorganismes, ERL 6300 CNRS, UMR1347 INRA/Agrosup/Université de Bourgogne Agroécologie, 17 Rue Sully, BP 86510, 21065, Dijon Cedex, France

    Leonardo Casieri & Daniel Wipf

  2. Pôle GEAPSI, UMR1347 INRA/Agrosup/Université de Bourgogne Agroécologie, BP 86510, 21065, Dijon Cedex, France

    Karine Gallardo

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  1. Leonardo Casieri
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Correspondence to Leonardo Casieri.

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Casieri, L., Gallardo, K. & Wipf, D. Transcriptional response of Medicago truncatula sulphate transporters to arbuscular mycorrhizal symbiosis with and without sulphur stress. Planta 235, 1431–1447 (2012). https://doi.org/10.1007/s00425-012-1645-7

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