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Tyramine accumulation in rice cells caused a dwarf phenotype via reduced cell division

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Abstract

Transgenic rice plants overexpressing a rice tyrosine decarboxylase (TyDC) exhibited a dwarf phenotype with a high level of tyramine accumulation. The height of transgenic rice was reduced on average to 35% of the wild-type height, whereas the number of tillers increased to 190% that of wild type. When judged by cellular distribution of tyramine and tyramine derivatives, the level of tyramine in soluble and insoluble fractions was higher than that of tyramine derivatives such as 4-coumaroyltyramine (CT) in the transgenic rice plants, suggesting that tyramine rather than its derivatives was a causative compound triggering the dwarf phenotype. Microscopic observation revealed that cell size in the transgenic lines was maintained, with a slightly irregular arrangement in the leaf mesophyll cells. When wild-type rice seeds were grown in the presence of tyramine, rice seedlings also showed stunted phenotypes in a dose-dependent manner. When these stunted seedlings were employed to measure the degree of cellular proliferation by bromodeoxyuridine incorporation, only small numbers of cells were found to retain labeled nuclei in shoot tips compared with the untreated control. These results show that the dwarf phenotype associated with tyramine accumulation in transgenic rice plants is attributable to a reduction in cell number rather than cell size. In addition, our dwarf phenotype caused by tyramine was not closely associated with known dwarf genes such as D88.

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Abbreviations

TYDC:

l-Tyrosine decarboxylase

FT:

Feruloyltyramine

CT:

4-Coumaroyltyramine

HPLC:

High performance liquid chromatography

RT-PCR:

Reverse transcription polymerase chain reaction

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Acknowledgments

This research was supported by the Basic Science Research Programme through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0087207) and by the Priority Research Centres Programme through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology (2010-0020141).

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Authors and Affiliations

  1. Department of Biotechnology, Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Bioenegry Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, South Korea

    Young Soon Kim, Sangkyu Park, Kiyoon Kang, Kyungjin Lee & Kyoungwhan Back

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  1. Young Soon Kim
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  2. Sangkyu Park
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  3. Kiyoon Kang
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Correspondence to Kyoungwhan Back.

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Kim, Y.S., Park, S., Kang, K. et al. Tyramine accumulation in rice cells caused a dwarf phenotype via reduced cell division. Planta 233, 251–260 (2011). https://doi.org/10.1007/s00425-010-1303-x

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  • DOI: https://doi.org/10.1007/s00425-010-1303-x

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