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Over-expression of AGPase genes enhances seed weight and starch content in transgenic maize

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Abstract

Cereal crops accumulate starch in the seed endosperm as an energy reserve. ADP-glucose pyrophosphorylase (AGPase) plays a key role in regulating starch biosynthesis in cereal seeds. The AGPase in the maize endosperm is a heterotetramer of two small subunits, encoded by Brittle2 (Bt2) gene, and two large subunits, encoded by the Shrunken2 (Sh2) gene. The two genes (Bt2, Sh2) from maize were introduced into two elite maize inbred lines, solely and in tandem, and under the control of endosperm-specific promoters for over-expression. PCR, Southern blotting, and real-time RT-PCR analysis indicated that the transgenes were integrated into the genome of transgenic plants and were over-expressed in their progeny. The over-expression of either gene enhanced AGPase activity, seed weight and starch content compared with the WT, but the amounts were lower than plants with over-expression of both Bt2 and Sh2. Developing seeds from co-expression transgenic maize plants had higher cytoplasmic AGPase activity: the 100-grain weight increased 15% over the wild type (WT), and the starch content increased to over 74% compared with the WT of 65%. These results indicate that over-expression of the genes in transgenic maize plants could improve kernel traits. This report provides a feasible approach for increasing starch content and seed weight in maize.

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Abbreviations

AGPase:

ADP-glucose pyrophosphorylase

ADPG:

ADP-glucose

Bt2:

Brittle2

CTAB:

Cetyltrime thylammonium bromide

DAP:

Days after pollination

DW:

Dry weight

FW:

Fresh weight

G-1-P:

Glucose-1-phosphate

G6PDH:

Glucose-6-phosphate dehydrogenase

PGM:

Phosphoglucomutase

Sh2:

Shrunken 2

WT:

Wild type

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Acknowledgments

This research was supported by National Basic Research Program of China (973 Program 2009CB118400) and the National Program of Transgenic Variety Development of China (2009ZX08003-001B). The authors thank Dr. Roberta Greenwood for her help in editing this manuscript.

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Authors and Affiliations

  1. School of Life Science, Shandong University, 27 Shanda South Road, Jinan, 250100, People’s Republic of China

    Ning Li, Shujuan Zhang, Yajie Zhao, Bei Li & Juren Zhang

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  1. Ning Li
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  2. Shujuan Zhang
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  4. Bei Li
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  5. Juren Zhang
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Correspondence to Juren Zhang.

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Li, N., Zhang, S., Zhao, Y. et al. Over-expression of AGPase genes enhances seed weight and starch content in transgenic maize. Planta 233, 241–250 (2011). https://doi.org/10.1007/s00425-010-1296-5

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