Skip to main content

Advertisement

SpringerLink
Log in

Comparative proteomic analysis of longan (Dimocarpus longan Lour.) seed abortion

  • Original Article
  • Published:
Planta Aims and scope Submit manuscript

Abstract

Two-dimensional gel electrophoresis (2-DE), coupled with mass spectroscopy, was used to study seed abortion in Dimocarpus longan Lour. (cv. Minjiao 64-1) by comparing normal and aborted seeds at three developmental stages. More than 1,000 protein spots were reproducibly detected in 2-DE gels, with 43 protein spots being significantly altered in their intensity between normal and aborted seeds at least at one stage. Thirty-five proteins were identified by matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS) analysis and protein database searching. Most of the identified proteins were associated with a variety of functions, including energy and metabolism (30%), programed cell death (9%), antioxidative processes (14%), chaperonin (23%), cell division, amino acid metabolism, secondary metabolism, and other functional classes. Furthermore, the expression patterns of HSP70 and cytosolic ascorbate peroxidase (cAPX) were validated by immunoblotting analysis. This study provides a novel, global insight into proteomic differences between normal and aborted seeds in longan. We anticipate that identification of the differentially expressed proteins may lead to a better understanding of the molecular basis for seed abortion in longan.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

DAP:

Days after pollination

2-DE:

Two-dimensional gel electrophoresis

MALDI-TOF-MS/MS:

Matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry

IEF:

Isoelectric focusing

IPG:

Immobilized pH gradient

pI:

Isoelectric point

PCD:

Programed cell death

ROS:

Reactive oxygen species

APX:

Ascorbate peroxidase

PTM:

Post-translational modification

References

  • Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399

    Article  PubMed  CAS  Google Scholar 

  • Arathi HS, Ganeshaiah KN, Shaanker RU, Hegde SG (1999) Seed abortion in Pongamia pinnata (Fabaceae). Am J Bot 86:659–662

    Article  PubMed  Google Scholar 

  • Baba AI, Nogueira FCS, Pinheiro CB, Brasil JN, Jereissati ES, Juca TL, Soares AA, Santos MF, Domont GB, Campos FAP (2008) Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta). Plant Sci 175:717–723

    Article  CAS  Google Scholar 

  • Beers EP, Woffenden BJ, Zhao C (2000) Plant proteolytic enzymes: possible roles during programmed cell death. Plant Mol Biol 44:399–415

    Article  PubMed  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 7:248–254

    Article  Google Scholar 

  • Chen Z, Gallie DR (2005) Increasing tolerance to ozone by elevating foliar ascorbic acid confers greater protection against ozone than increasing avoidance. Plant Physiol 138:1673–1689

    Article  PubMed  CAS  Google Scholar 

  • Dam S, Laursen BS, Ornfelt JH, Jochimsen B, Staerfeldt HH, Friis C, Nielsen K, Goffard N, Besenbacher S, Krusell L, Sato S, Tabata S, Thogersen IB, Enghild JJ, Stougaard J (2009) The proteome of seed development in the model legume Lotus japonicus. Plant Physiol 149:1325–1340

    Article  PubMed  CAS  Google Scholar 

  • Dat JF, Pellinen R, Beeckman T, Van De Cotte B, Langebartels C, Kangasjarvi J, Inze D, Van Breusegem F (2003) changes in hydrogen peroxide homeostasis trigger an active cell death process in tobacco. Plant J 33:621–632

    Article  PubMed  CAS  Google Scholar 

  • Ellis RJ, Van der Vies SM (1988) The rubisco subunit binding protein. Photosynth Res 16:101–115

    Article  CAS  Google Scholar 

  • Finnie C, Melchior S, Roepstorff P, Svensson B (2002) Proteome analysis of grain filling and seed maturation in barley. Plant Physiol 129:1308–1319

    Article  PubMed  CAS  Google Scholar 

  • Franks RG, Wang C, Levin JZ, Liu Z (2002) SEUSS, a member of a novel family of plant regulatory proteins, represses floral homeotic gene expression with LEUNIG. Development 129:253–263

    PubMed  CAS  Google Scholar 

  • Gallardo K, Le Signor C, Vandekerckhove J, Thompson RD, Burstin J (2003) Proteomics of Medicago truncatula seed development establishes the time frame of diverse metabolic processes related to reserve accumulation. Plant Physiol 133:664–682

    Article  PubMed  CAS  Google Scholar 

  • Gallie DR, Le H, Caldwell C, Tanguay RL, Hoang NX, Browning KS (1997) The phosphorylation state of translation initiation factors is regulated developmentally and following heat shock in wheat. J Biol Chem 272:1046–1053

    Article  PubMed  CAS  Google Scholar 

  • Ghazoul J, Satake A (2009) Nonviable seed set enhances plant fitness: the sacrificial sibling hypothesis. Ecology 90:369–377

    Article  PubMed  Google Scholar 

  • Hajduch M, Ganapathy A, Stein JW, Thelen JJ (2005) A systematic proteomic study of seed filling in soybean: establishment of high resolution two-dimensional reference maps, expression profiles, and an interactive proteome database. Plant Physiol 137:1397–1419

    Article  PubMed  CAS  Google Scholar 

  • Hajduch M, Casteel JE, Hurrelmeyer KE, Song Z, Agrawal GK, Thelen JJ (2006) Proteomic analysis of seed filling in Brassica napus. Developmental characterization of metabolic isozymes using high-resolution two-dimensional gel electrophoresis. Plant Physiol 141:32–46

    Article  PubMed  CAS  Google Scholar 

  • Hashimoto M, Komatsu S (2007) Proteomic analysis of rice seedlings during cold stress. Proteomics 7:1293–1302

    Article  PubMed  CAS  Google Scholar 

  • Hauser BA, Sun K, Oppenheimer DG, Sage TL (2006) Changes in mitochondrial membrane potential and accumulation of reactive oxygen species precede ultrastructural changes during ovule abortion. Planta 223:492–499

    Article  PubMed  CAS  Google Scholar 

  • Helenurm K, Schaal BA (1996) Genetic load, nutrient limitation, and seed production in Lupinus texensis (Fabaceae). Am J Bot 83:1585–1595

    Article  Google Scholar 

  • Ho SL, Tong WF, Yu SM (2000) Multiple mode regulation of a cysteine proteinase gene expression in rice. Plant Physiol 122:57–66

    Article  PubMed  CAS  Google Scholar 

  • Hossaert M, Valero M (1988) Effect of ovule position in the pod on patterns of seed formation in two species of Lathyrus (Leguminosae: Papilionoideae). Am J Bot 75:1714–1731

    Article  Google Scholar 

  • Ishikawa A, Tanaka H, Nakai M, Asahi T (2003) Deletion of a chaperonin 60 beta gene leads to cell death in the Arabidopsis lesion initiation 1 mutant. Plant Cell Physiol 44:255–261

    Article  PubMed  CAS  Google Scholar 

  • Jones-Rhoades MW, Borevitz JO, Preuss D (2007) Genome-wide expression profiling of the Arabidopsis female gametophyte identifies families of small, secreted proteins. PLoS Genet 3:1848–1861

    Article  PubMed  CAS  Google Scholar 

  • Locato V, de Pinto MC, De Gara L (2009) Different involvement of the mitochondrial, plastidial and cytosolic ascorbate-glutathione redox enzymes in heat shock responses. Physiol Plant 135:296–306

    Article  PubMed  CAS  Google Scholar 

  • Marshall DL, Levin DA, Fowler NL (1985) Plasticity in yield components in response to fruit predation and date of fruit initiation in three species of Sesbania (Leguminosae). J Ecol 73:71–81

    Article  Google Scholar 

  • Marsoni M, Bracale M, Espen L, Prinsi B, Negri AS, Vannini C (2008) Proteomic analysis of somatic embryogenesis in Vitis vinifera. Plant Cell Rep 27:347–356

    Article  PubMed  CAS  Google Scholar 

  • Mechin V, Thevenot C, Le Guilloux M, Prioul JL, Damerval C (2007) Developmental analysis of maize endosperm proteome suggests a pivotal role for pyruvate orthophosphate dikinase. Plant Physiol 143:1203–1219

    Article  PubMed  CAS  Google Scholar 

  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

    Article  PubMed  CAS  Google Scholar 

  • Miyajima D (2006) Ovules that failed to form seeds in zinnia (Zinnia violacea Cav). Sci Hortic 107:176–182

    Article  Google Scholar 

  • Nadeau JA, Zhang XS, Li J, O’Neill SD (1996) Ovule development: identification of stage-specific and tissue-specific cDNAs. Plant Cell 8:213–239

    Article  PubMed  CAS  Google Scholar 

  • Nelson RJ, Ziegelhoffer T, Nicolet C, Werner-Washburne M, Craig EA (1992) The translation machinery and 70 kd heat shock protein cooperate in protein synthesis. Cell 71:97–105

    Article  PubMed  CAS  Google Scholar 

  • Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279

    Article  PubMed  CAS  Google Scholar 

  • Patterson BD, MacRae EA, Ferguson IB (1984) Estimation of hydrogen peroxide in plant extracts using titanium (IV). Anal Biochem 139:487–492

    Article  PubMed  CAS  Google Scholar 

  • Puigderrajols P, Jofre A, Mir G, Pla M, Verdaguer D, Huguet G, Molinas M (2002) Developmentally and stress-induced small heat shock proteins in cork oak somatic embryos. J Exp Bot 373:1445–1452

    Article  Google Scholar 

  • Qureshi MI, Qadir S, Zolla L (2007) Proteomics-based dissection of stress-responsive pathways in plants. J Plant Physiol 164:1239–1260

    Article  PubMed  CAS  Google Scholar 

  • Roe JL, Nemhauser JL, Zambryski PC (1997) TOUSLED participates in apical tissue formation during gynoecium development in Arabidopsis. Plant Cell 9:335–353

    Article  PubMed  CAS  Google Scholar 

  • Saravanan RS, Rose JK (2004) A critical evaluation of sample extraction techniques for enhanced proteomics analysis of recalcitrant plant tissues. Proteomics 4:2522–2532

    Article  PubMed  CAS  Google Scholar 

  • Seavy SR, Carter SK (1996) Ovule fates in Epilobium obcordatum (Onagraceae). Am J Bot 83:316–325

    Article  Google Scholar 

  • Shulaev V, Oliver DJ (2006) Metabolic and proteomic markers for oxidative stress. New tools for reactive oxygen species research. Plant Physiol 141:367–372

    Article  PubMed  CAS  Google Scholar 

  • Sin SF, Yeung EC, Chye ML (2006) Downregulation of Solanum americanum genes encoding proteinase inhibitor II causes defective seed development. Plant J 45:58–72

    Article  PubMed  CAS  Google Scholar 

  • Skinner DJ, Hill TA, Gasser CS (2004) Regulation of ovule development. Plant Cell 16:S32–S45

    Article  PubMed  CAS  Google Scholar 

  • Stephenson AG (1981) Flower and fruit abortion: proximate causes and ultimate functions. Annu Rev Ecol Syst 12:253–279

    Article  Google Scholar 

  • Sun K, Hunt K, Hauser BA (2004) Ovule abortion in Arabidopsis triggered by stress. Plant Physiol 135:2358–2367

    Article  PubMed  CAS  Google Scholar 

  • Sun K, Cui Y, Hauser BA (2005) Environmental stress alters genes expression and induces ovule abortion: reactive oxygen species appear as ovules commit to abort. Planta 222:632–642

    Article  PubMed  CAS  Google Scholar 

  • Teixeira FK, Menezes-Benavente L, Galvão VC, Margis R, Margis-Pinheiro M (2006) Rice ascorbate peroxidase gene family encodes functionally diverse isoforms localized in different subcellular compartments. Planta 224:300–314

    Article  PubMed  CAS  Google Scholar 

  • Thibaud-Nissen F, Shealy RT, Khanna A, Vodkin LO (2003) Clustering of microarray data reveals transcript patterns associated with somatic embryogenesis in soybean. Plant Physiol 132:118–136

    Article  PubMed  CAS  Google Scholar 

  • Tischner T, Allphin L, Chase K, Orf JH, Lark KG (2003) Genetics of seed abortion and reproductive traits in soybean. Crop Sci 43:464–473

    Article  CAS  Google Scholar 

  • Ueda T, Seo S, Ohashi Y, Hashimoto J (2000) Circadian and senescence-enhanced expression of a tobacco cysteine protease gene. Plant Mol Biol 44:649–657

    Article  PubMed  CAS  Google Scholar 

  • Vacca RA, de Pinto MC, Valenti D, Passarella S, Marra E, De Gara L (2004) Production of reactive oxygen species, alteration of cytosolic ascorbate peroxidase, and impairment of mitochondrial metabolism are early events in heat shock-induced programmed cell death in tobacco Bright-Yellow 2 cells. Plant Physiol 134:1100–1112

    Article  PubMed  CAS  Google Scholar 

  • Van Breusegem F, Dat JF (2006) Reactive oxygen species in plant cell death. Plant Physiol 141:384–390

    Article  PubMed  CAS  Google Scholar 

  • Villanueva JM, Broadhvest J, Hauser BA, Meister RJ, Schneitz K, Gasser CS (1999) INNER NO OUTER regulates abaxial-adaxial patterning in Arabidopsis ovules. Genes Dev 13:3160–3169

    Article  PubMed  CAS  Google Scholar 

  • Wang TL, Hadavizideh A, Harwood A, Welham TJ, Harwood WA, Faulks R, Hedley CL (1990) An analysis of seed development in Pisum sativum. XIII. The chemical induction of storage product mutants. Plant Breed 105:311–320

    Article  CAS  Google Scholar 

  • Wang J, Zhang H, Allen RD (1999) Overexpression of an Arabidopsis peroxisomal ascorbate peroxidase gene in tobacco increases protection against oxidative stress. Plant Cell Physiol 40:725–732

    PubMed  CAS  Google Scholar 

  • Wang XQ, Yan PF, Gao Q, Liu XL, Kuang TY, Shen SH, He YK (2008) Proteomic analysis of the response to high-salinity stress in Physcomitrella patens. Planta 228:167–177

    Article  PubMed  CAS  Google Scholar 

  • Watson BS, Asirvatham VS, Wang L, Sumner LW (2003) Mapping the proteome of barrel medic (Medicago truncatula). Plant Physiol 131:1104–1123

    Article  PubMed  Google Scholar 

  • Wehmeyer N, Vierling E (2000) The expression of small heat shock proteins in seeds responds to discrete developmental signals and suggests a general protective role in desiccation tolerance. Plant Physiol 122:1099–1108

    Article  PubMed  CAS  Google Scholar 

  • Wei WH, Chen B, Yan XH, Wang LJ, Zhang HF, Cheng JP, Zhou XA, Sha AH, Shen H (2008) Identification of differentially expressed genes in soybean seeds differing in oil content. Plant Sci 175:663–673

    Article  CAS  Google Scholar 

  • Weretilnyk EA, Hanson AD (1990) Molecular cloning of a plant betaine-aldehyde dehydrogenase, an enzyme implicated in adaptation to salinity and drought. Proc Natl Acad Sci USA 87:2745–2749

    Article  PubMed  CAS  Google Scholar 

  • Wiederanders B (2003) Structure-function relationship in class CA1 cysteine peptidase propeptides. Acta Biochim Polonica 50:691–713

    CAS  Google Scholar 

  • Winkelmann T, Heintz D, Van Dorsselaer A, Serek M, Braun HP (2006) Proteomic analyses of somatic and zygotic embryos of Cyclamen persicum Mill. reveal new insights into seed and germination physiology. Planta 224:508–519

    Article  PubMed  CAS  Google Scholar 

  • Wolucka BA, Van Montagu M (2003) GDP-mannose 3′, 5′-epimerase forms GDP-l-gulose, a putative intermediate for the de novo biosynthesis of vitamin C in plants. J Biol Chem 278:47483–47490

    Article  PubMed  CAS  Google Scholar 

  • Xu FX, Chye ML (1999) Expression of cysteine proteinase during developmental events associated with programmed cell death in brinjal. Plant J 17:321–327

    Article  PubMed  CAS  Google Scholar 

  • Yang PF, Chen H, Liang Y, Shen SH (2007) Proteomic analysis of de-etiolated rice seedlings upon exposure to light. Proteomics 7:2459–2468

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Grant of China (Award no. 30370999) and the Provincial Natural Science Grant of Fujian, PR China (Award no. 2009J01084). We also would like to thank Professor Jonathan E. Poulton of the Department of Biology at The University of Iowa (USA) for the critical reviewing of the manuscript.

Author information

Authors and Affiliations

  1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, 350002, Fuzhou, China

    Hao Liu, Yan-zhen Liu & Wei Chen

  2. College of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China

    Hao Liu, Yan-zhen Liu & Wei Chen

  3. Fruit Research Institute, Fujian Academy of Agricultural Sciences, 350013, Fuzhou, China

    Shao-quan Zheng & Ji-mou Jiang

  4. College of Horticulture Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China

    Ping Wang

Authors
  1. Hao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan-zhen Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shao-quan Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ji-mou Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wei Chen.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplemental Figure S1 (PDF 216 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, H., Liu, Yz., Zheng, Sq. et al. Comparative proteomic analysis of longan (Dimocarpus longan Lour.) seed abortion. Planta 231, 847–860 (2010). https://doi.org/10.1007/s00425-009-1093-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00425-009-1093-1

Keywords

Search

Navigation