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Hormonal regulation of the basic peroxidase isoenzyme from Zinnia elegans

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Abstract

Xylem differentiation in plants is under strict hormonal regulation. Auxins and cytokinins, together with brassinosteroids (BRs), appear to be the main hormones controlling vascular differentiation. In this report, we study the effect of these hormones on the basic peroxidase isoenzyme from Zinnia elegans (ZePrx), an enzyme involved in lignin biosynthesis. Results showed that auxins and cytokinins induce ZePrx, similarly to the way in which they induce seedling secondary growth (in particular, metaxylem differentiation). Likewise, the exogenous application of BR reduces the levels of ZePrx, in a similar way to their capacity to inhibit seedling secondary growth. Consistent with this notion, the exogenous application of BR reverses the auxin/cytokinin-induced ZePrx expression, but has no effect on the auxin/cytokinin-induced secondary growth. This differential hormonal response is supported by the analysis of the ZePrx promoter, which contains (a) cis-elements directly responsive to these hormones and (b) cis-elements targets of the plethora of transcription factors, such as NAC, MYB, AP2, MADS and class III HD Zip, which are up-regulated during the auxin- and cytokinin-induced secondary growth. Taken together, these results suggest that ZePrx is directly and indirectly regulated by the plethora of hormones that control xylem differentiation, supporting the role of ZePrx in xylem lignification.

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Abbreviations

ARFs:

Auxin-response factors

ARR1:

Arabidopsis response regulator 1

Aux/IAA:

Auxin/indole-3-acetic acid

AuxREs:

Auxin-responsive cis-elements

BAP:

6-Benzylaminopurine

BES1:

BR-responsive bri1-EMS-suppressor 1

BRs:

Brassinosteroids

BZR1:

BR-responsive brassinazole-resistant 1

EB:

20-epi-Brassinolide

NAA:

1-Naphthaleneacetic acid

SAUR :

Small auxin-up RNA

ZePrx:

Basic peroxidase isoenzyme from Zinnia elegans

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Acknowledgments

This work was supported by grants from the MEC (BFU2006-11577/BFI)-FEDER and CARM (08610/PI/08). JG holds a fellowship (FPI) from the MCYT.

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Authors and Affiliations

  1. Department of Plant Biology, University of La Coruña, 15071, La Coruña, Spain

    Jorge Gutiérrez, Esther Novo Uzal & José Díaz

  2. Department of Plant Biology, University of Murcia, 30100, Murcia, Spain

    María Josefa López Núñez-Flores, Laura V. Gómez-Ros, Juan Cuello & Alfonso Ros Barceló

  3. Department of Plant Biology, University of Alcalá, 28871, Alcalá de Henares, Spain

    Alberto Esteban Carrasco

  4. IBMC, University of Porto, 4150, Porto, Portugal

    Mariana Sottomayor

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  1. Jorge Gutiérrez
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  2. María Josefa López Núñez-Flores
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  3. Laura V. Gómez-Ros
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  4. Esther Novo Uzal
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  5. Alberto Esteban Carrasco
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  8. Juan Cuello
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  9. Alfonso Ros Barceló
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Correspondence to Alfonso Ros Barceló.

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Gutiérrez, J., López Núñez-Flores, M.J., Gómez-Ros, L.V. et al. Hormonal regulation of the basic peroxidase isoenzyme from Zinnia elegans . Planta 230, 767–778 (2009). https://doi.org/10.1007/s00425-009-0982-7

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