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Two different effects of calcium on aquaporins in salinity-stressed pepper plants

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Abstract

Two different effects of calcium were studied, respectively, in plasma membrane vesicles and in protoplasts isolated from roots of control pepper plants (Capsicum annuum L cv. California) or of plants treated with 50 mM NaCl, 10 mM CaCl2 or 10 mM CaCl2 + 50 mM NaCl. Under saline conditions, osmotic water permeability (P f ) values decreased in protoplasts and plasma membrane vesicles, and the same reduction was observed in the PIP1 aquaporin abundance, indicating inhibitory effects of NaCl on aquaporin functionality and protein abundance. The cytosolic Ca2+ concentration, [Ca2+]cyt, was reduced by salinity, as observed by confocal microscope analysis. Two different actions of Ca2+ were observed. On the one hand, increase in free cytosolic calcium concentrations associated with stress perception may lead to aquaporin closure. On the other hand, when critical requirements of Ca2+ were reduced (by salinity), and extra-calcium would lead to an upregulation of aquaporins, indicating that a positive role of calcium at whole plant level combined with an inhibitory mechanism at aquaporin level may work in the regulation of pepper root water transport under salt stress. However, a link between these observations and other cell signalling in relation to water channel gating remains to be established.

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Abbreviations

DTT:

Dithiothreitol

BSA:

Bovine serum albumine

SDS:

Sodium dodecyl sulphate

DAB:

3,3′Diaminobenzidine

DMSO:

Dimethyl sulfoxide

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Acknowledgments

The authors thank Prof. Dr. Schäffner (Institute of Biochemical Plant Pathology, GSF—National Research Center for Environment and Health, Neuherberg, Germany), for providing the AtPIP1 antibody, Dr. L. Verdoucq and L. López, for technical assistance in vesicles P f measurements, and Dr. D. Walker (Dpto. de Cultivos No AlimentariosIMIDA—Murcia, Spain), for correction of the English in the manuscript. This work was supported by the European Commission Research Directorate General Human Resources and Mobility (Marie Curie).

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Authors and Affiliations

  1. Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura—CSIC, Apdo. Correos 164, 30100, Espinardo, Murcia, Spain

    M. Carmen Martínez-Ballesta, Francisco Cabañero, Enrique Olmos & Micaela Carvajal

  2. Departamento de Ingeniería de Alimentos y del Equipamiento Agrícola, Universidad Politécnica de Cartagena, Cartagena, Murcia, Spain

    Paula María Periago

  3. Biochimie et Physiologie Moléculaire des Plantes SupAgro/INRA/CNRS/UM2, Montpellier, France

    Christophe Maurel

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  1. M. Carmen Martínez-Ballesta
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  2. Francisco Cabañero
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  3. Enrique Olmos
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  4. Paula María Periago
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  5. Christophe Maurel
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  6. Micaela Carvajal
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Correspondence to Micaela Carvajal.

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Martínez-Ballesta, M.C., Cabañero, F., Olmos, E. et al. Two different effects of calcium on aquaporins in salinity-stressed pepper plants. Planta 228, 15–25 (2008). https://doi.org/10.1007/s00425-008-0714-4

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  • DOI: https://doi.org/10.1007/s00425-008-0714-4

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