Skip to main content
SpringerLink
Log in

A RAV-like transcription factor controls photosynthesis and senescence in soybean

  • Original Article
  • Published:
Planta Aims and scope Submit manuscript

Abstract

A cDNA library enriched for mRNAs encoding ESTs that increased in abundance during short days was constructed by SSH from leaf tissues of a photoperiod sensitive soybean. The proteins predicted to be encoded by the mRNAs were inferred to be involved in diverse functions. A full-length mRNA that encoded a soybean ortholog of the transcription factor RAV was isolated by RACE, containing an open reading frame of 1,056 bp. The GmRAV protein included an AP2/ERF domain and a B3 domain. GmRAV mRNA abundance was increased in SDs following leaf treatments with ABA and decreased following BR treatment. Transgenic tobacco overexpressing GmRAV showed morphological and physiological alterations such as slower plant growth rate (dwarfing), reduced root elongation, delayed flowering time and reduced photosynthetic rate, reduced chlorophyll contents in leaves. Therefore GmRAV may be a negative regulator acting on both photosynthesis and growth. Transgenic tobacco also showed accelerated senescence with both dark and ABA treatments versus the longer longevity compared to the wild type in LDs. The analyses of soybean leaf, root and stem organs showed that GmRAV mRNA abundances were higher in SDs than in LDs. Therefore, the enhanced expression of GmRAV in SDs compared to LDs may have caused the inhibited growth of soybean leaf, root and stem.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

ABA:

Abscisic acid

6-BA:

6-Benzyladenine

epiBL:

epiBrassinolide

BR:

Brassinsteroid

GmRAV:

Soybean RAV-like DNA-binding protein

LD:

Long-day

RACE:

Rapid amplification of cDNA ends

SD:

Short-day

SSH:

Suppression subtractive hybridization

References

  • Asamizu E, Nakamura Y, Sato S, Tabata S (2000) A large scale analysis of cDNA in Arabidopsis thaliana: generation of 12028 non-redundant expressed sequence tags from normalized and size-selected cDNA libraries. DNA Res 7:175–180

    Article  PubMed  Google Scholar 

  • Buchanan-Wollaston V, Earl S, Harrison E, Mathas E, Navabpour S, Page T, Pink D (2003) The molecular analysis of leaf senescence—a genomics approach. Plant Biotechnol J 1:3–22

    Article  PubMed  CAS  Google Scholar 

  • Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu S-H, Swidzinski J, Ishizaki K, Leaver CJ (2005) Comparative transcriptome analysis reveals significant differences in gene expression and signaling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J 42:567–585

    Article  PubMed  CAS  Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Fehr WR (1987) Principles of cultivar development. In: Theory and technique, vol. 1. Macmillan, New York

  • Fehr WR, Caviness CE (1977) Stages of soybean development. Iowa Agricultural Experimental Station Special Report, p 80

  • Finkelstein RR, Wang ML, Lynch TJ, Rao S, Goodman HM (1998) The Arabidopsis abscisic acid response locus ABI4 encodes an APETALA 2 domain protein. Plant Cell 10:1043–1054

    Article  PubMed  CAS  Google Scholar 

  • Gan S, Amasino RM (1997) Making sense of senescence. Molecular genetic regulation and manipulation of leaf senescence. Plant Physiol 113:313–319

    PubMed  CAS  Google Scholar 

  • Gepstein S, Sabehi G, Carp MJ, Hajouj T, Nesher MFO, Yariv I, Dor C, Bassani M (2003) Large-scale identification of leaf senescence-associated genes. Plant J 36:629–642

    Article  PubMed  CAS  Google Scholar 

  • Grant D, Cregan P, Shoemaker RC (2000) Genome organization in dicots: genome duplication in Arabidopsis and synteny between soybean and Arabidopsis. Proc Natl Acad Sci USA 97:4168–4173

    Article  PubMed  CAS  Google Scholar 

  • Gutterson N, Reuber TL (2004) Regulation of disease resistance pathways by AP2/ERF transcription factors. Curr Opin Plant Biol 7:1–7

    Article  Google Scholar 

  • Han T, Wu C, Tong Z, Mentreddy RS, Tan K, Gai J (2006) Postflowering photoperiod regulates vegetative growth and reproductive development of soybean. Environ Exp Bot 55:120–129

    Article  Google Scholar 

  • Horsch RB, Fry JE, Hoffman NC, Eicholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transforming gene into plants. Science 227:1229–1231

    Article  CAS  Google Scholar 

  • Iqbal MJ, Yaegashi S, Njiti VN, Ahsan R, Cryder KL, Lightfoot DA (2002) Resistance locus pyramids alter transcript abundance in soybean roots inoculated with Fusarium solani f.sp. glycines. Mol Genet Genomic 268:407–417

    Article  CAS  Google Scholar 

  • Kagaya Y, Ohmiya K, Hattori T (1999) RAV1, a novel DNA binding protein, binds top bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plants. Nucleic Acids Res 27:470–478

    Article  PubMed  CAS  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382

    Article  CAS  Google Scholar 

  • Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Venter JC et al (1999) Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana. Nature 402:761–768

    Article  PubMed  CAS  Google Scholar 

  • Njiti VN, Lightfoot DA (2006) Genetic analysis infers Dt loci underlie resistance to SDS caused by Fusarium virguliforme in indeterminate soybeans. Can J Plant Sci 41:83–89

    Google Scholar 

  • Riechmann JL, Meyerowitz EM (1998) The AP2/EREBP family of plant transcription factors. Biol Chem 379:633–646

    Article  PubMed  CAS  Google Scholar 

  • Shoemaker R, Keim P, Vodkin L, Retzel E, Clifton SW et al (2002) A compilation of soybean ESTs: generation and analysis. Genome 45:329–338

    Article  PubMed  Google Scholar 

  • Shultz JL, Ray JD, Lightfoot DA (2007) A sequence based synteny map between soybean and Arabidopsis thaliana. BMC Genomics 8:8. doi:10.1186/1471-2164-8-8

    Article  PubMed  CAS  Google Scholar 

  • Simpson GG, Dean C (2002) Arabidopsis, the rosetta stone of flowering time? Science 296:285–289

    Article  PubMed  CAS  Google Scholar 

  • Smart CM (1994) Gene expression during leaf senescence. New Phytol 129:419–448

    Article  Google Scholar 

  • Smart CM, Hosken SE, Thomas H, Greaves JA, Blair BG, Schuch W (1995) The timing of maize leaf senescence and characterization of senescence-related cDNAs. Physiol Plant 93:673–682

    Article  CAS  Google Scholar 

  • Ulmasov T, Hagen G, Guifoyle TJ (1997) ARF1, a transcription factor that binds to an auxin responsive elements. Science 276:1865–1868

    Article  PubMed  CAS  Google Scholar 

  • Yuan Z, Njiti V, Meksem K, Iqbal MJ, Triwitayakorn K, Kassem MA, Davis GT, Schmidt ME, Lightfoot DA (2002) Identification of yield loci in soybean populations that segregate for disease resistance. Crop Sci 42:271–277

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This project was conducted in the Key Laboratory of Soybean Biology of Chinese Education Ministry and financially supported by Natural Scientific Foundation Projects, National High Technology (863) Projects (contract no. 2006AA100104-4 and 2006AA10Z1F1) and National 973 Project. The technical assistance of Dr. Genlou Sun is gratefully acknowledged.

Author information

Authors and Affiliations

  1. Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agriculture University, 150030, Harbin, China

    Lin Zhao, Qiulan Luo, Yingpeng Han & Wenbin Li

  2. The Basic Medical Science College, Harbin Medical University, 150081, Harbin, China

    Chunliang Yang

Authors
  1. Lin Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qiulan Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chunliang Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yingpeng Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wenbin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wenbin Li.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhao, L., Luo, Q., Yang, C. et al. A RAV-like transcription factor controls photosynthesis and senescence in soybean. Planta 227, 1389–1399 (2008). https://doi.org/10.1007/s00425-008-0711-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00425-008-0711-7

Keywords

Search

Navigation