Reversible inhibition of I _K, I _AHP, I _h and I _Ca currents by internally applied gluconate in rat hippocampal pyramidal neurones

Abstract

 Previously, we reported that the spike frequency adaptation and slow afterhyperpolarizations (sAHP) in hippocampal pyramidal neurones are best preserved during whole-cell recording with a methylsulfate (MeSO₄ ^–)- based internal solution, but undergo a fast rundown when gluconate- (Gluc^–)- based internal solution is used. Here we show, with internal perfusion of patch pipettes, the reversibility of the inhibitory effects of Gluc^–on spike frequency adaptation and sAHP, and extend these observations to fast and medium-duration AHPs. Contrary to what might be expected based on Gluc^–binding of Ca²⁺, the sAHP and its underlying current could be temporarily enhanced by adding 1–3 mM of the calcium chelator BAPTA to the internal solution in the presence of Gluc^–. Replacement of internal MeSO₄ ^–with Gluc^–did not affect the membrane resting potential or the amplitude and duration of action potentials, but reversibly increased the cell input resistance and decreased the threshold current for spike generation. Gluc^–reversibly inhibited the hyperpolarization-activated non-selective cationic current (I _h), the depolarization-activated delayed rectifier K⁺ current (I _K), the high-voltage-activated Ca²⁺ current and the Ca²⁺-activated K⁺ current that underlies the sAHP. The combination of these effects of Gluc^–significantly alters the electrophysiological ”fingerprint” of the neurone.