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The sources of calcium for noradrenaline-induced contraction in the human thoracic internal artery

  • Muscle Physiology
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Abstract

The aim of the present study was to examine the contribution of intracellular and extracellular calcium sources in contraction caused by noradrenaline (NA) of the human internal thoracic artery (ITA) in vitro. Distal segments of ITA were obtained from 20 patients (aged 38–73, at the time of routine coronary artery surgical revascularization (CABG)). Contractile responses to 10−6 mol/L NA in the physiological salt solution and in Ca2+-free solution without and after incubation with 10−6 mol/L thapsigargin (TSG) were recorded under isometric conditions. Responses of ITA rings to 1 μM NA without incubation with TSG accounted (% of reaction to 80 mM KCl) 224.70 ± 14.06% in PSS solution, 141.30 ± 8.66% in Ca2+-free solution, and 80.03 ± 1.71% after PSS restoration and were statistically significantly different (p < 0.0001, one-way ANOVA). Responses of ITA rings to 1 μM NA with 1 μM TSG accounted (% of reaction to 80 mM KCl) 114.50 ± 2.79% in Ca2+-free solution and 36.70 ± 2.38% after PSS restoration. Responses in Ca2+-free solution and after PSS restoration without and with TSG were statistically significantly different (p = 0.0257 and p < 0.0001, respectively—t test). ITA contraction is caused by calcium derived not only from the SR and the extracellular matrix. The delivery of calcium to the space surrounding tissue does not immediately deliver calcium to the myofilaments.

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Authors and Affiliations

  1. Department of Cardiac Surgery, Medical University of Białystok, Białystok, Poland

    Leszek Buzun

  2. Department of Cardiac Surgery, The Regional Specialist Hospital, Olsztyn, Poland

    Leszek Buzun

  3. Department of Biophysics, Medical University of Bialystok, ul. Mickiewicza 2A, 15-222, Białystok, Poland

    Beata Modzelewska, Anna Kostrzewska & Tomasz Kleszczewski

  4. Łomża Medical College of the Universal Educational Society, Łomża, Poland

    Anna Kostrzewska

  5. Institute of Health Care, Higher Vocational School, Suwałki, Poland

    Ewa Kleszczewska

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  1. Leszek Buzun
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  2. Beata Modzelewska
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  4. Ewa Kleszczewska
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  5. Tomasz Kleszczewski
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Corresponding author

Correspondence to Tomasz Kleszczewski.

Ethics declarations

The study protocol was reviewed and approved by the local committee of ethics (the approval reference number – R-I-003/42/2002). The study conformed to the Declaration of Helsinki.

Sources of funding

This study was supported by the Medical University of Białystok, Award Number 3-16634.

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The authors declare that they have no conflict of interest.

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Buzun, L., Modzelewska, B., Kostrzewska, A. et al. The sources of calcium for noradrenaline-induced contraction in the human thoracic internal artery. Pflugers Arch - Eur J Physiol 469, 1135–1140 (2017). https://doi.org/10.1007/s00424-017-1982-2

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  • DOI: https://doi.org/10.1007/s00424-017-1982-2

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