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Enhanced lateral inhibition in the barrel cortex by deletion of phospholipase C-related catalytically inactive protein-1/2 in mice

  • Ion channels, receptors and transporters
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Abstract

We previously demonstrated that the deletion of phospholipase C-related catalytically inactive protein-1/2 (PRIP-1/2) enhances the desensitization of GABAA receptors (GABAARs), while it facilitates their resensitization at the offset of GABA puff, causing a hump-like tail current (tail-I) in layer 3 (L3) pyramidal cells (PCs) of the barrel cortex. In the present study, we investigated whether inhibitory synaptic transmission in L3 PCs in the barrel cortex is altered in the PRIP-1/2 double-knockout (PRIP-DKO) mice, and if so, how the interaction between excitation and inhibition is subsequently modified. PRIP-1/2 deletion resulted in the prolongation of the decay phase of inhibitory postsynaptic currents/potentials (IPSCs/IPSPs) in L3 PCs evoked by stimulation of L3, leaving the overall features of miniature IPSCs unchanged. An optical imaging revealed that the spatiotemporal profile of a horizontal excitation spread across columns in L2/3 caused by L4 stimulation in the barrel cortex was more restricted in PRIP-DKO mice compared to the wild type, while those obtained in the presence of bicuculline were almost identical between the two genotypes. These findings suggest that PRIP-1/2 deletion enhances the lateral inhibition by prolonging inhibitory synaptic actions to limit the intercolumnar integration in the barrel cortex. Considering the present findings together with our previous study including a mathematical simulation, the prolongation of inhibitory synaptic actions is likely to result from an enhancement of desensitization followed by an enhanced resensitization in GABAARs.

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Acknowledgments

This study was supported by a Grant-in-Aid for Scientific Research to Y.K. (B; 26290006) and by the Japan Ministry of Education, Culture, Sports, Science and Technology to M.H. (S; 24229009).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Neuroscience and Oral Physiology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka, 565-0871, Japan

    Hiroki Toyoda, Mitsuru Saito, Hajime Sato, Tsutomu Kawano, Shinpei Kawakami & Youngnam Kang

  2. Nourishment Function Laboratory, Health Care Division, Morinaga & Co., Ltd., Yokohama, Kanagawa, 230-8504, Japan

    Shinpei Kawakami

  3. Department of Fixed Prosthodontics, Osaka University Graduate School of Dentistry, Suita, Osaka, 565-0871, Japan

    Hirofumi Yatani

  4. Department of Dental Pharmacology, Division of Integrated Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, 734-8553, Japan

    Takashi Kanematsu

  5. Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science, Kyushu University, Fukuoka, 812-8582, Japan

    Masato Hirata

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  1. Hiroki Toyoda
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  2. Mitsuru Saito
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Correspondence to Youngnam Kang.

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Toyoda, H., Saito, M., Sato, H. et al. Enhanced lateral inhibition in the barrel cortex by deletion of phospholipase C-related catalytically inactive protein-1/2 in mice. Pflugers Arch - Eur J Physiol 467, 1445–1456 (2015). https://doi.org/10.1007/s00424-014-1592-1

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  • DOI: https://doi.org/10.1007/s00424-014-1592-1

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