Abstract
The distal parts of the renal tubule play a critical role in maintaining homeostasis of extracellular fluids. In this review, we present an in-depth analysis of microarray-based gene expression profiles available for microdissected mouse distal nephron segments, i.e., the distal convoluted tubule (DCT) and the connecting tubule (CNT), and for the cortical portion of the collecting duct (CCD; Zuber et al., Proc Natl Acad Sci USA 106:16523–16528, 2009). Classification of expressed transcripts in 14 major functional gene categories demonstrated that all principal proteins involved in maintaining the salt and water balance are represented by highly abundant transcripts. However, a significant number of transcripts belonging, for instance, to categories of G-protein-coupled receptors or serine/threonine kinases exhibit high expression levels but remain unassigned to a specific renal function. We also established a list of genes differentially expressed between the DCT/CNT and the CCD. This list is enriched by genes related to segment-specific transport functions and by transcription factors directing the development of the distal nephron or collecting ducts. Collectively, this in silico analysis provides comprehensive information about relative abundance and tissue specificity of the DCT/CNT and the CCD expressed transcripts and identifies new candidate genes for renal homeostasis.
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This work was supported by the Swiss National Science Foundation Research Grant 3100A0-117824 (DF) and a bridge grant for young investigator from the Faculty of Biology and Medicine of the University of Lausanne and the Prof. Placide Nicod Foundation (OB). We would like to thank Dr. Hannes Richter from the Lausanne Genomic Technologies facility for the qPCR analysis of GPCRs expression levels.
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Olivier Bonny and Dmitri Firsov have equally contributed to the study.
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Pradervand, S., Zuber Mercier, A., Centeno, G. et al. A comprehensive analysis of gene expression profiles in distal parts of the mouse renal tubule. Pflugers Arch - Eur J Physiol 460, 925–952 (2010). https://doi.org/10.1007/s00424-010-0863-8
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DOI: https://doi.org/10.1007/s00424-010-0863-8