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Differential contribution of cardiac sarcomeric proteins in the myofibrillar force response to stretch

  • Cardiovascular Physiology
  • Published:
Pflügers Archiv - European Journal of Physiology Aims and scope Submit manuscript

Abstract

The present study examined the contribution of myofilament contractile proteins to regional function in guinea pig myocardium. We investigated the effect of stretch on myofilament contractile proteins, Ca2+ sensitivity, and cross-bridge cycling kinetics (K tr) of force in single skinned cardiomyocytes isolated from the sub-endocardial (ENDO) or sub-epicardial (EPI) layer. As observed in other species, ENDO cells were stiffer, and Ca2+ sensitivity of force at long sarcomere length was higher compared with EPI cells. Maximal K tr was unchanged by stretch, but was higher in EPI cells possibly due to a higher α-MHC content. Submaximal Ca2+-activated K tr increased only in ENDO cells with stretch. Stretch of skinned ENDO muscle strips induced increased phosphorylation in both myosin-binding protein C and myosin light chain 2. We concluded that transmural MHC isoform expression and differential regulatory protein phosphorylation by stretch contributes to regional differences in stretch modulation of activation in guinea pig left ventricle.

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Acknowledgements

This work was supported by the “Association Française contre les Myopathies”, and “Région Languedoc-Roussillon”. OC is an established investigator of CNRS. PPT was supported by INSERM and NIH grants HL-62426; HL-75494; HL-77195. Thanks are due to Guillermo Salazar for technical assistance.

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Authors and Affiliations

  1. INSERM, U 637, Université MONTPELLIER I, UFR de Médecine, F-34295, Montpellier, France

    Younss Ait mou, Pieter P. de Tombe & Olivier Cazorla

  2. INSERM E0211, F-37032, Tours, France

    Jean-Yves le Guennec & Emilio Mosca

  3. Center for Cardiovascular Research, University of Illinois, Chicago, IL, 60612, USA

    Pieter P. de Tombe

  4. Physiopathologie Cardiovasculaire, INSERM U637, CHU Arnaud de Villeneuve, 34295, Montpellier Cedex 5, France

    Olivier Cazorla

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  1. Younss Ait mou
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  2. Jean-Yves le Guennec
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  3. Emilio Mosca
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  4. Pieter P. de Tombe
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  5. Olivier Cazorla
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Correspondence to Olivier Cazorla.

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Ait mou, Y., le Guennec, JY., Mosca, E. et al. Differential contribution of cardiac sarcomeric proteins in the myofibrillar force response to stretch. Pflugers Arch - Eur J Physiol 457, 25–36 (2008). https://doi.org/10.1007/s00424-008-0501-x

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  • DOI: https://doi.org/10.1007/s00424-008-0501-x

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