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Impact of mechanical stress on ion transport in native lung epithelium (Xenopus laevis): short-term activation of Na+, Cl− and K+ channels

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Abstract

Epithelia, in general, and the lung epithelium, in particular, are exposed to mechanical forces, but little is known about their impact on pulmonary ion transport. In our present study, we employed transepithelial ion transport measurements on Xenopus lung preparations using custom-built Ussing chambers. Tissues were exposed to mechanical stress by increasing the water column (5 cm) at one side of the tissues. Apical exposure to hydrostatic pressure significantly decreased the short circuit current (I SC: 24 ± 1%, n = 152), slightly decreased the transepithelial resistance (R T: 7 ± 2%, n = 152), but increased the apical membrane capacitance (C M: 16 ± 6%, n = 9). The pressure-induced effect was sensitive to Na+ (amiloride), Cl− (DIDS, NFA, NPPB) and K+ channel blockers (Ba2+, glibenclamide). Further on, it was accompanied by increased extracellular ATP levels. The results show that mechanical stress leads to an activation of Na+, Cl−, and K+ conductances in a native pulmonary epithelium resulting in a net decrease of ion absorption. This could be of considerable interest, since an altered ion transport may contribute to pathophysiological conditions, e.g., the formation of pulmonary edema during artificial ventilation.

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Acknowledgements

The authors gratefully acknowledge the experimental and technical assistance of M. Buss. Also, we would like to thank S. Kristek for his technical support and especially for providing the modified Ussing chambers and B. Kahnert for electrical support. All experiments were in agreement with the German law of animal care (permission provided by the regional board Giessen).

Grants

The present study was supported by the Deutsche Forschungsgemeinschaft grant # FR 2124/1-1.

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  1. Institute of Animal Physiology, Justus-Liebig University Giessen, Wartweg 95, 35392, Giessen, Germany

    Roman Bogdan, Christine Veith, Wolfgang Clauss & Martin Fronius

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  1. Roman Bogdan
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  4. Martin Fronius
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Correspondence to Martin Fronius.

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Bogdan, R., Veith, C., Clauss, W. et al. Impact of mechanical stress on ion transport in native lung epithelium (Xenopus laevis): short-term activation of Na+, Cl− and K+ channels. Pflugers Arch - Eur J Physiol 456, 1109–1120 (2008). https://doi.org/10.1007/s00424-008-0486-5

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  • DOI: https://doi.org/10.1007/s00424-008-0486-5

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