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Endurance training attenuates exercise-induced oxidative stress in erythrocytes in rat

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Abstract

The aim of this study was to investigate whether endurance training reduces exercise-induced oxidative stress in erythrocytes. Male rats (n=54) were divided into trained (n=28) and untrained (n=26) groups. Both groups were further divided equally into two groups where the rats were studied at rest and immediately after exhaustive exercise. Endurance training consisted of treadmill running 1.5 h·day−1, 5 days a week for 8 weeks, reaching the speed of 2.1 km·h−1 at the fourth week. For acute exhaustive exercise, graded treadmill running was conducted reaching the speed of 2.1 km·h−1 at the 95th min, 10% uphill, and was continued until exhaustion. Acute exhaustive exercise increased the erythrocyte malondialdehyde level in sedentary but not in trained rats compared with the corresponding sedentary rest and trained rest groups, respectively. While acute exhaustive exercise decreased the erythrocyte superoxide dismutase activity in sedentary rats, it increased the activity of this enzyme in trained rats. On the other hand, acute exhaustive exercise increased the erythrocyte glutathione peroxidase activity in sedentary rats; however, it did not affect this enzyme activity in trained rats. Erythrocyte glutathione peroxidase activity was higher in trained groups compared with untrained sedentary group. Neither acute exhaustive exercise nor treadmill training affected the erythrocyte total glutathione level. Treadmill training increased the endurance time in trained rats compared with sedentary rats. The results of this study suggest that endurance training may be useful to prevent acute exhaustive exercise-induced oxidative stress in erythrocytes by up-regulating some of the antioxidant enzyme activities and may have implications in exercising humans.

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References

  • Alessio HM (1993) Exercise-induced oxidative stress. Med Sci Sports Exerc 25:218–224

    CAS  PubMed  Google Scholar 

  • Anuradha CV, Balakrishnan SD (1998) Effect of training on lipid peroxidation, thiol status and antioxidant enzymes in tissues of rats. Indian J Physiol Pharmacol 42:64–70

    CAS  PubMed  Google Scholar 

  • Atalay M, Laaksonen DE, Niskanen L, Uusitupa M, Hanninen O, Sen CK (1997) Altered antioxidant enzyme defences in insulin-dependent diabetic men with increased resting and exercise-induced oxidative stress. Acta Physiol Scand 161:195–201

    Article  CAS  PubMed  Google Scholar 

  • Clemens MR, Waller HD (1987) Lipid peroxidation in erythrocytes. Chem Phys Lipids 45:251–268

    CAS  PubMed  Google Scholar 

  • Davies KJ, Quintanilha AT, Brooks GA, Packer L (1982) Free radicals and tissue damage produced by exercise. Biochem Biophys Res Commun 107:1198–1205

    CAS  PubMed  Google Scholar 

  • Duthie GG, Robertson JD, Maughan RJ, Morrice PC (1990) Blood antioxidant status and erythrocyte lipid peroxidation following distance running. Arch Biochem Biophys 282:78–83

    CAS  PubMed  Google Scholar 

  • Gonenc S, Acikgoz O, Semin I, Ozgonul H (2000) The effect of moderate swimming exercise on antioxidant enzymes and lipid peroxidation levels in children. Indian J Physiol Pharmacol 44:340–344

    CAS  PubMed  Google Scholar 

  • Gul M, Hänninen O (2002) Physiological basis of exercise. In: Hänninen O (ed) Physiology and maintenance. Encyclopedia of Life Support Systems (EOLSS). Developed under the Auspices of the UNESCO and Eolss, Oxford (http://www.eolss.net)

  • Gul M, Kutay FZ, Temocin S, Hanninen O (2000) Cellular and clinical implications of glutathione. Indian J Exp Biol 38:625–634

    CAS  PubMed  Google Scholar 

  • Gul M, Oztasan N, Taysi S, Gumustekin K, Akar S, Bakan N, Dane S (2001) Short-term swimming exercise as an oxidative stress model in rat [in Turkish]. Hacettepe J Sport Sci 12:26–32

    Google Scholar 

  • Gul M, Laaksonen DE, Atalay M, Vider L, Hanninen O (2002) Effects of endurance training on tissue glutathione homeostasis and lipid peroxidation in streptozotocin-induced diabetic rats. Scand J Med Sci Sports 12:163–170

    Article  PubMed  Google Scholar 

  • Gul M, Atalay M, Hänninen O (2003) Endurance training and glutathione-dependent antioxidant defense mechanism in heart of the diabetic rats. J Sports Sci Med 2:52–61 (available from http://www.jssm.org)

  • Halliwell B, Gutteridge JMC (1984) Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy. Lancet 1:1396–1397

    CAS  PubMed  Google Scholar 

  • Jain SK, McVie R, Duett J, Herbst JJ (1989) Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes 38:1539–1543

    CAS  PubMed  Google Scholar 

  • Jenkins RR (1988) Free radical chemistry. Relationship to exercise. Sports Med 5:156–170

    CAS  PubMed  Google Scholar 

  • Ji LL (1993) Antioxidant enzyme response to exercise and aging. Med Sci Sports Exerc 25:225–231

    CAS  PubMed  Google Scholar 

  • Kanter MM, Hamlin RL, Unverferth DV, Davis HW, Merola AJ (1985) Effect of exercise training on antioxidant enzymes and cardiotoxicity of doxorubicin. J Appl Physiol 59:1298–1303

    CAS  PubMed  Google Scholar 

  • Kanter MM, Nolte LA, Holloszy JO (1993) Effects of an antioxidant vitamin mixture on lipid peroxidation at rest and postexercise. J Appl Physiol 74:965–969

    CAS  PubMed  Google Scholar 

  • Khanna S, Atalay M, Laaksonen DE, Gul M, Roy S, Sen CK (1999) Alpha-lipoic acid supplementation: tissue glutathione homeostasis at rest and after exercise. J Appl Physiol 86:1191–1196

    Article  CAS  PubMed  Google Scholar 

  • Leeuwenburgh C, Fiebig R, Chandwaney R, Ji LL (1994) Aging and exercise training in skeletal muscle: responses of glutathione and antioxidant enzyme systems. Am J Physiol 267:R439–R445

    CAS  PubMed  Google Scholar 

  • Marzatico F, Pansarasa O, Bertorelli L, Somenzini L, Della Valle G (1997) Blood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances in highly trained aerobic and sprint athletes. J Sports Med Phys Fitness 37:235–239

    CAS  PubMed  Google Scholar 

  • Michiels C, Raes M, Toussaint O, Remacle J (1994) Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress. Free Radic Biol Med 17:235–248

    CAS  PubMed  Google Scholar 

  • Miyazaki H, Oh-ishi S, Ookawara T, Kizaki T, Toshinai K, Ha S, Haga S, Ji LL, Ohno H (2001) Strenuous endurance training in humans reduces oxidative stress following exhausting exercise. Eur J Appl Physiol 84:1–6

    Google Scholar 

  • Ohno H, Yahata T, Sato Y, Yamamura K, Taniguchi N (1988) Physical training and fasting erythrocyte activities of free radical scavenging enzyme systems in sedentary men. Eur J Appl Physiol 57:173–176

    CAS  Google Scholar 

  • Paglia DE, Valentina WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    CAS  PubMed  Google Scholar 

  • Sahlin K, Ekberg K, Cizinsky S (1991) Changes in plasma hypoxanthine and free radical markers during exercise in man. Acta Physiol Scand 142:275–281

    CAS  PubMed  Google Scholar 

  • Sen CK (1995) Oxidants and antioxidants in exercise. J Appl Physiol 79:675–686

    CAS  PubMed  Google Scholar 

  • Sen CK (1997) Nutritional biochemistry of cellular glutathione. J Nutr Biochem 8:660–672

    Article  CAS  Google Scholar 

  • Sen CK, Marin E, Kretzschmar M, Hanninen O (1992) Skeletal muscle and liver glutathione homeostasis in response to training, exercise, and immobilization. J Appl Physiol 73:1265–1272

    CAS  PubMed  Google Scholar 

  • Senturk UK, Gunduz F, Kuru O, Aktekin MR, Kipmen D, Yalcin O, Bor-Kucukatay M, Yesilkaya A, Baskurt OK (2001) Exercise-induced oxidative stress affects erythrocytes in sedentary rats but not exercise-trained rats. J Appl Physiol 91:1999–2004

    Google Scholar 

  • Siems WG, Sommerburg O, Grune T (2000) Erythrocyte free radical and energy metabolism. Clin Nephrol 53:S9–S17

    CAS  PubMed  Google Scholar 

  • Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500

    CAS  PubMed  Google Scholar 

  • Tauler P, Gimeno I, Aguiló A, Guix MP, Pons A (1999) Regulation of erythrocyte antioxidant enzyme activities in athletes during competition and short-term recovery. Pflugers Arch 438:782–787

    CAS  Google Scholar 

  • Tietze F (1969) Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 27:502–522

    CAS  PubMed  Google Scholar 

  • Tiidus PM (1998) Radical species in inflammation and overtraining. Can J Physiol Pharmacol 76:533–538

    PubMed  Google Scholar 

  • Tiidus PM, Houston ME (1994) Antioxidant and oxidative enzyme adaptations to vitamin E deprivation and training. Med Sci Sports Exerc 26:354–359

    CAS  PubMed  Google Scholar 

  • Tiidus PM, Pushkarenko J, Houston ME (1996) Lack of antioxidant adaptation to short-term aerobic training in human muscle. Am J Physiol 271:R832–R836

    CAS  PubMed  Google Scholar 

  • Venditti P, Di Meo S (1996) Antioxidants, tissue damage, and endurance in trained and untrained young male rats. Arch Biochem Biophys 331:63–68

    Article  CAS  PubMed  Google Scholar 

  • Yalcin O, Bor-Kucukatay M, Senturk UK, Baskurt OK (2000) Effects of swimming exercise on red blood cell rheology in trained and untrained rats. J Appl Physiol 88:2074–2080

    Google Scholar 

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Acknowledgements

This study has been supported by The Research Foundation of the Ataturk University (project number: 2001/53), Erzurum, Turkey. The authors thank Delali Camgoz, who took care of the animals.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Ataturk University, Erzurum, Turkey

    Nuray Oztasan, Kenan Gumustekin, Omer Aktas, Handan Timur, Sedat Akar, Senol Dane & Mustafa Gul

  2. Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey

    Seyithan Taysi, Konca Altinkaynak, Sait Keles & Fatih Akcay

  3. College of Physical Education and Sports, Ataturk University, 25240, Erzurum, Turkey

    Erdinc Siktar

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  1. Nuray Oztasan
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  2. Seyithan Taysi
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  3. Kenan Gumustekin
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  12. Mustafa Gul
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Correspondence to Mustafa Gul.

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Oztasan, N., Taysi, S., Gumustekin, K. et al. Endurance training attenuates exercise-induced oxidative stress in erythrocytes in rat. Eur J Appl Physiol 91, 622–627 (2004). https://doi.org/10.1007/s00421-003-1029-6

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  • DOI: https://doi.org/10.1007/s00421-003-1029-6

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