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The monocarboxylate transporters exist in the cattle endocrine pancreas

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Abstract

Extensive studies are published concerning the distribution of monocarboxylate transporters (MCTs) in various animal issues including ruminants; nonetheless, nothing is known about their cellular expression and localization in the ruminant pancreas. The present study was carried out to examine the expression and cellular localization of all the fourteen MCT isoforms in cattle pancreas. RT-PCR verified the existence of mRNA transcripts for eight MCT isoforms, namely, MCT1, MCT2, MCT3, MCT4, MCT5, MCT8, MCT13, and MCT14 in cattle pancreas. Western blotting analysis confirmed the protein expression of these eight MCTs in the cattle pancreatic tissue. Immunohistochemical study, within the whole pancreas, was conducted to localize the eight MCTs identified, and the results showed strong positive immunoreactive staining for MCT1, MCT2, MCT4, MCT5, MCT13, and MCT14 on nearly all the islet cells of Langerhans, while we could not detect immunopositive signals in the acinar cells with any of MCTs antibodies used. This study, for the first time, showed the cellular localization and expression of MCT1–MCT5, MCT8, MCT13, and MCT14 within the ruminant pancreas. The distribution and expression pattern of MCT1, MCT2, MCT4, and CD147 in the cattle pancreas are different from that previously published on monogastric pancreas. Our study suggested that MCT1, MCT2, MCT4, MCT5, MCT13, and MCT14 may participate in the regulation of the pancreatic endocrine secretions in ruminants.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

    Doaa Kirat

  2. Department of Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan

    Doaa Kirat & Seiyu Kato

Authors
  1. Doaa Kirat
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  2. Seiyu Kato
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Correspondence to Doaa Kirat.

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418_2014_1271_MOESM1_ESM.jpg

Representative RT-PCR analysis for the negative expression of some MCT isoforms in the cattle pancreas (e.g., MCT6, MCT7, MCT9, and MCT10). DNA ladders (NewEngland BioLabs) were included as reference for fragment size

418_2014_1271_MOESM2_ESM.jpg

Immunoblots for the expression of MCT3 and MCT8 in the cattle pancreas. Bands of approximately 45 and 50 kDa were barely detected in the cattle pancreas immunoblots corresponding to the protein of MCT3 and MCT8, respectively. Protein marker size (kDa) is indicated on the left

418_2014_1271_MOESM3_ESM.jpg

Cellular locations of MCT3 and MCT8 in the cattle pancreas. Positive cells are barely detected in the pancreatic islets cells only. Scale bar, 30 μm

Primer sequences used for amplification of the monocarboxylate transporter family in the cattle pancreas

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Kirat, D., Kato, S. The monocarboxylate transporters exist in the cattle endocrine pancreas. Histochem Cell Biol 143, 185–194 (2015). https://doi.org/10.1007/s00418-014-1271-5

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